LOCUS       sequence-261781-        2861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNeonGreen monomeric fluorescent protein expression for mammalian 
            cells under EF1a-HTLV promoter in minimal backbone plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2861)
  TITLE     Constitutive promoter minimal backbone plasmids for optimized 
            expression in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EF1-alpha-mNeonGreen-Z1
FEATURES             Location/Qualifiers
     source          1..2861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..265
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             278..546
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      610..619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             616..1326
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      871..880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1378..1499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1389..1408)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1443..1462
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1658..2032)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2042..2060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2052..2098)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(2171..2759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2251..2270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcagaagc tttactaact agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga
       61 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
      121 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      181 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      241 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
      301 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
      361 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
      421 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
      481 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
      541 cagatccaag ctgtgaccgg cgcctacaat tctgcagtcg acggtaccgc gggcccggga
      601 tccaccggtg ccaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg
      661 acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag
      721 ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac
      781 ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg
      841 ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggctaccaa
      901 gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc
      961 tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac
     1021 ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggtcgaa gaagacttac
     1081 cccaacgaca aaaccatcat cagtaccttt aagtggagtt acaccactgg aaatggcaag
     1141 cgctaccgga gcactgcgcg gaccacctac acctttgcca agccaatggc ggctaactat
     1201 ctgaagaacc agccgatgta cgtgttccgt aagacggagc tcaagcactc caagaccgag
     1261 ctcaacttca aggagtggca aaaggccttt accgatgtga tgggcatgga cgagctgtac
     1321 aagtaatagg cggccgcgac tctagatgta cacatatggc tagctggcca gacatgataa
     1381 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1441 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1501 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1561 aaagcaagta aaacctctac aaatgtggta tggaattctg gactcacaaa gaaaaaacgc
     1621 ccggtgtgca agaccgagcg ttctgaacaa tttatcctca gtcctgctcc tctgccacaa
     1681 agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc
     1741 cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc
     1801 actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca
     1861 ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga
     1921 agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc
     1981 cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc atgatggccc
     2041 tcctatagtg agtcgtatta tactatgccg atatactatg ccgatgatta attgtcaagg
     2101 atcctaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2161 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2221 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2281 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2341 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2401 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2461 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2521 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2581 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2641 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2701 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2761 aagatccttt gatcttttct acggggtctg acgctcagtg aaaactcacg ttaagggatt
     2821 ttggtcatgg ctagttaatt aacatttaaa tcagcggccg c
//
