LOCUS       sequence-261782-        2853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AausFP1 - bright green fluorescent protein expression from A. 
            australis under EF1a-HTLV promoter in minimal backbone plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2853)
  TITLE     Constitutive promoter minimal backbone plasmids for optimized 
            expression in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EF1-alpha-AausFP1-Z1
FEATURES             Location/Qualifiers
     source          1..2853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..265
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             278..546
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      611..620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1370..1491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1381..1400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1435..1454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1650..2024)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2034..2052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2044..2090)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(2163..2751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2243..2262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcagaagc tttactaact agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga
       61 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
      121 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      181 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      241 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
      301 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
      361 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
      421 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
      481 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
      541 cagatccaag ctgtgaccgg cgcctacaat tctgcagtcg acggtaccgc gggcccggga
      601 tccaccggtc gccaccatgg gttcctatgg tgctctgctg tttagggaga agatacctta
      661 cgtggtcgaa atggaaggcg acgtcgaagg catgaaattc agcgtgcggg gcaaggggca
      721 cggagatgcc aatacaggga agatcgaggc ttcctttata tgtactaccg gcgagctgcc
      781 tgttccctgg agctctatac tcaccaccgt gacctatggt gcccaatgct tcgctaagta
      841 cccgaacgac ataaaggatt atcctaagtc cgcaatgccc gaggggtatg tccaggaacg
      901 taccataaca ttcgagaatg atggagttta caaaacgcgg gccgaggtta cttatgaaaa
      961 gggctccgtc tacaataggg tgacactgaa tgggtccggg ttcaagaagg gcggaaatat
     1021 tctgggaaag aagctggagt tcaattataa tccccactgt atttacgtcc tgcccgacgt
     1081 tcagaacaac ggcattaagt gttatattaa tatcgtgcac gatgtgatcg gcggcggtca
     1141 gatcatcgca gcccatcagc agctgaacac accactcggc ggcggtcccg tagatatccc
     1201 gcattatcac cacatccagg cccataccat cctctcaaag gacccgaagg agaccaggga
     1261 ccacatgaat gtggtcgaag tatttcgtgc cattgactgt aagactgctt acgcctgata
     1321 ggcggccgcg actctagatg tacacatatg gctagctggc cagacatgat aagatacatt
     1381 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1441 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1501 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1561 taaaacctct acaaatgtgg tatggaattc tggactcaca aagaaaaaac gcccggtgtg
     1621 caagaccgag cgttctgaac aatttatcct cagtcctgct cctctgccac aaagtgcacg
     1681 cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg
     1741 gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg
     1801 tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg
     1861 tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc
     1921 tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg
     1981 tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatgatggc cctcctatag
     2041 tgagtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa ggatcctaag
     2101 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2161 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2281 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2341 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2401 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2461 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2521 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2581 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2641 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2701 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2761 ttgatctttt ctacggggtc tgacgctcag tgaaaactca cgttaaggga ttttggtcat
     2821 ggctagttaa ttaacattta aatcagcggc cgc
//
