LOCUS       sequence-261793-        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of SpyTag-B2AR in insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Choi HK, Min D, Kang H, Shon MJ, Rah SH, Kim HC, Jeong H, Choi HJ, 
            Bowie JU, Yoon TY
  TITLE     Watching helical membrane proteins fold reveals a common 
            N-to-C-terminal folding pathway.
  JOURNAL   Science. 2019 Nov 29;366(6469):1150-1156. doi: 
            10.1126/science.aaw8208.
  PUBMED    31780561
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-11-29- 29"; volume: "366"; 
            issue: "6469"; pages: "1150-1156"
COMMENT     Sequence Label: pFastBac SpyTag-beta-2AR-SpyTag
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..69
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             1093..1131
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 gactacaagg acgatgatga cgccatcgat gctcatattg tcatggttga tgcttacaag
       61 ccaactaaga cgcagcaaag ggacgaggtg tgggtggtgg gcatgggcat cgtcatgtct
      121 ctcatcgtcc tggccatcgt gtttggcaat gtgctggtca tcacagccat tgccaagttc
      181 gagcgtctgc agacggtcac caactacttc atcacttcac tggcctgtgc tgatctggtc
      241 atgggcctgg cagtggtgcc ctttggggcc gcccacattc ttacgaaaac gtggactttt
      301 ggcaacttct ggtgcgagtt ttggacttcc attgatgtgc tgtgcgtcac ggccagcatt
      361 gagaccctgt gcgtgatcgc agtggatcgc tactttgcca ttacttcacc tttcaagtac
      421 cagagcctgc tgaccaagaa taaggcccgg gtgatcattc tgatggtgtg gattgtgtca
      481 ggccttacct ccttcttgcc cattcagatg cactggtacc gggccaccca ccaggaagcc
      541 atcaactgct atgccgagga gacatgttgt gacttcttca cgaaccaagc ctatgccatt
      601 gcctcttcca tcgtgtcctt ctacgttccc ctggtgatca tggtcttcgt ctactccagg
      661 gtctttcagg aggccaaaag gcagctccag aaaatcgaca agtctgaggg ccgcttccat
      721 gtccagaacc ttagccaggt ggagcaggat gggcggacgg ggcatggact ccgcagatct
      781 tccaagttct gcttgaagga gcacaaagcc ctcaagacgt taggcatcat catgggcact
      841 ttcaccctct gctggctgcc cttcttcatc gttaacattg tgcatgtgat ccaggataac
      901 ctcatccgta aggaagttta catcctccta aattggatag gctatgtcaa ttctggtttc
      961 aatcccctta tctactgccg gagcccagat ttcaggattg ccttccagga gcttctgtgc
     1021 ctgcgcaggt cttctttgaa ggcctatggg aatggctact ccagcaacgg caacacaggg
     1081 gagcagagtg gagctcatat tgtcatggtt gatgcttaca agccaactaa ggctagcggc
     1141 ctcgaagtcc ttttccag
//
