LOCUS       sequence-261798-        4470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO1B7
FEATURES             Location/Qualifiers
     source          1..4470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2595..2694)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2711..2730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2712..2730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2735..2751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2735..2751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2853..2872)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2864..3673
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2931..2950)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3820..4408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4309..4328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaaga tctccaccac acagatcgag cggagattcg agatcagctc
      721 ctctctggtg ggcctgatcg acggctcttt tgagatcggc aacctgtttg tgatcgtgtt
      781 cgtgtcttac tttggcagca agctgcacag gccaaagctg atcggcatcg gctgcttcct
      841 gatgggaacc ggaagcatcc tgatggcact gccccacttc tttatgggct actatcgcta
      901 tagcaaggag accaacatcg atccttccga gaatagcaca tccaacctgc caaattgcct
      961 gatcaaccag atgctgagcc tgaataggac cccatccgag atcatcgaga ggggatgcgt
     1021 gaaggagtct ggcagccaca tgtggatcta cgtgttcatg ggcaacatgc tgagaggcat
     1081 cggcgagaca cccatcgtgc ctctgggcat cagctacatc gacgattttg ccaaggaggg
     1141 ccacagctcc ctgtatctgg gcaccgtgaa tgtgatgggc atgacaggcc tggtgtttgc
     1201 cttcatgctg ggctccctgt tcgccaagat gtacgtggac atcggctatg tggatctgtc
     1261 taccatccgg atcacaccca aggacagcag atgggtggga gcatggtggc tgggctttct
     1321 ggtgtccggc atcgtgtcta tcatctctag catccctttc tttttcctgc cactgaaccc
     1381 caataagcct cagaaggagc ggaaggtgag cctgttcctg cacgtgctga agaccaacga
     1441 taagagaaat cagatcgcca acctgacaaa taggcgcaag tacatcacca agaacgtgac
     1501 aggctttttc cagtccctga agtctatcct gaccaatccc ctgtatgtga tctttgtgat
     1561 cttcacactg ctgcacatgt cctcttacat cgcctccctg acctatatca tcaagatggt
     1621 ggagcagcag tacggctggt ccgcctctaa gacaaacttc ctgctgggcg tgctggccct
     1681 gcctgcagtg gcaatcggaa tgttctccgg aggatatatc atcaagaagt ttaagctgtc
     1741 tctggtgggc ctggccaagc tggccttttg ctccgccacc gtgcacctgc tgtctcaggt
     1801 gctgtacttt ttcctgatct gtgagagcaa gtccgtggcc ggcctgaccc tgacatacga
     1861 cggcaatagc ccagtgcggt cccacgtgga tgtgccactg tcttattgca acagcgagtg
     1921 caattgtgac gagtctcagt gggagcccgt gtgcggcaac aatggcatca cctacctgag
     1981 cccttgcctg gccggctgta agagctcctc tggcaacaag gagccaatcg tgttctacaa
     2041 ttgcagctgc gtggaagtga tcggcctgca gaacaagaat tattccgccc acctgggaga
     2101 gtgcccaagg gacgatgcat gtacccgcaa gagctacgtg tatttcgtga tccaggtgct
     2161 ggacgccttt ctgtgcgccg tgggcctgac atcttacagc gtgctggtca tccggatcgt
     2221 gcagcctgag ctgaaggccc tggccatcgg cttccactcc atgatcatga gatctctggg
     2281 cggcatcctg gtgccaatct attttggcgc cctgatcgat accacatgta tgaagtggag
     2341 caccaactcc tgcggagcaa ggggagcctg tagaatctac aatagcacat atctgggccg
     2401 ggcctttttc ggcctgaagg tggccctgat ctttcctgtg ctggtgctgc tgaccgtgtt
     2461 tatcttcgtg gtgaggaaga agtcccacgg caaggataca aaggtgctgg agaacgagcg
     2521 ccaggtcatg gacgaggcca acctggagtt cctgaatgat tctgagcact ttgtgccaag
     2581 cgccgaggag caggacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2641 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2701 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2761 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2821 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2881 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2941 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3001 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3061 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3121 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3181 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3241 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3301 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3361 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3421 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3481 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3541 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3601 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3661 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3721 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3961 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4201 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4441 ggccttttgc tggccttttg ctcacatgtt
//
