LOCUS       sequence-261800-        9499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]
FEATURES             Location/Qualifiers
     source          1..9499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(836..855)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1105..1123
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2745..2754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2907..3210
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3211..3414
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3365..3385
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3476..3485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3482..4183
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     protein_bind    complement(5435..5459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5487..6059
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    6256..6311
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6709..6726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6880..7468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6960..6979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7639..8430,8431..8499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8262..8281
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8500..8604)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8672..8690
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    9165..9220
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     intron          join(9417..9499,1..490)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 gtgtatatca ttgtaaccat aaagagaaag gcaggatgat gaccagggtg tagttgtttc
       61 taccaataag aatatttcca cgccagccag aatttatatg cagaaatatt ctaccttatc
      121 atttaattat aacaattgtt ctctaaaact gtgctgaagt acaatataat ataccctgat
      181 tgccttgaaa aaaaagtgat tagagaaagt acttacaatc tgacaaataa acaaaagtga
      241 atttaaaaat tcgttacaaa tgcaagctaa agtttaacga aaaagttaca gaaaatgaaa
      301 agaaaataag aggagacaat ggttgtcaac agagtagaaa gtgaaagaaa caaaattatc
      361 atgagggtcc atggtgatac aagggacatc ttcccattct aaacaacacc ctgaaaactt
      421 tgccccctcc atataacatg aattttacaa tagcgaaaaa gaaagaacaa tcaagggtcc
      481 ccaaactcac cctgaagttc tcagtctaga gtcgagagga tcaatcactt tgtacaagaa
      541 agctgggtct agatagctta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
      601 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
      661 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      721 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      781 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      841 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      901 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      961 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
     1021 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
     1081 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
     1141 taggggcggc cctgaggaaa aaaaaggaaa gggcatgctc gccatgtgca agatacagtg
     1201 ttgaatgtgg tgcaaaatat aaagatgtgg aagaaaaagc cactgaaaat aatatgagga
     1261 aataaaaaac tagagtaaat tatcagtgga acaatttcta ttatacaaga cactacaccc
     1321 atacattctc ctaatgctca tgtaagaatt tctccaaaaa taccttccag cactacaaac
     1381 tagaaaaaaa aagagacatg gacaccaaat taagttctta atagtgaaga acaaaagaac
     1441 taccttgcct gctccagtgg atccagcaac cgccaacaac tgtcctcttt ctatcttgaa
     1501 attaatatct ttcaggacag gagtaccaag aagtgagaaa ttactgaaga agaggctgtc
     1561 atcaccatta gaagtttttc tattgttatt gttttgtttt gctttctcaa ataattcccc
     1621 aaatccctgt taaaaaaaaa cacacacaca cacacacaca cacacatcaa aaataaaaga
     1681 tgagtttgtc agatattttc aatagatgct tgttttatgt accatactca cctcgccctc
     1741 gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat
     1801 gaactccttg atgatggcca tgttatcctc ctcgcccttg ctggttccgg tgcctccact
     1861 ttcatcttca cctttagtga taccggctgc cgtcacgaac tccttcagga ccatgtgatc
     1921 gcgcttctcg ttcgggtctt tgctcagcgt ggtctgggtg ctcagatagt ggttgtccgg
     1981 caggcgcacc gggccgtcgc cgatcggggt gttctgctgt ttgtggtcgg cttcctgctg
     2041 gctgccgtcc tcaacgttgt ggcggatctt gaagttagcc ttgatgccgt tcttctgctt
     2101 gtcggcttcg atacggacgt tgtggctgtt gtggttgtac tccagcttgt gacccaggat
     2161 gttgccgtcc tccttgaagt cgttgccctt cagctcgatg cggttcacca gggtgtcgcc
     2221 ctcgaacttc acctccgcgc gggtcttgta ggtgccgtcg tccttgaact cgatggtgcg
     2281 ctcctggacg tagccttccg gcatggcgga cttgaagaag tcgtgacgct tcatgtggtc
     2341 cgggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tcggccacgg
     2401 caccggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccct cggtggcatc
     2461 gccctcgccc tcgccacgca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2521 tttgataggc acagtacctg taaatagttc ctctccttta ctggtaccat catcagggaa
     2581 tgagcttaca tttctcctct tcagcatctg gtcatccttc ttagctttcc tcagctccac
     2641 attgacctct attctgcgac gcctcatttc tgtactgtct tttcccttgt tcttgaatct
     2701 gtgaagacgg gcagctggtg tattagcatt ctcgttggtg gatcccatgg tggcaccggt
     2761 gaattctcca ggcgatctga cggttcacta aacgagctct gcttatatag gcctcccacc
     2821 gtacacgcca cctcgacata ctcgagtagt tattaatagt aatcaattac ggggtcatta
     2881 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2941 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     3001 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     3061 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     3121 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     3181 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     3241 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     3301 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     3361 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     3421 gtgaaccgtc agatccgcta gggatcctct agtcagctga cgcgttacca ccggtgccac
     3481 catgggaagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     3541 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     3601 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     3661 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     3721 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     3781 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     3841 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     3901 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     3961 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag
     4021 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     4081 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     4141 cagatactgc gacctcccta gcaaactggg gcacaagctt aattccggaa tgtctgaata
     4201 tattcgggta accgaagatg agaacgatga gcccattgaa ataccatcgg aagacgatgg
     4261 gacggtgctg ctctccacgg ttacagccca gtttccaggg gcgtgtgggc ttcgctacag
     4321 gaatccagtg tctcagtgta tgagaggtgt ccggctggta gaaggaattc tgcatgcccc
     4381 agatgctggc tggggaaatc tggtgtatgt tgtcaactat ccaaaagata acaaaagaaa
     4441 aatggatgag acagatgctt catcagcagt gaaagtgaaa agagcagtcc agaaaacatc
     4501 cgatttaata gtgttgggtc tcccatggaa aacaaccgaa caggacctga aagagtattt
     4561 tagtaccttt ggagaagttc ttatggtgca ggtcaagaaa gatcttaaga ctggtcattc
     4621 aaaggggttt ggctttgttc gttttacgga atatgaaaca caagtgaaag taatgtcaca
     4681 gcgacatatg atagatggac gatggtgtga ctgcaaactt cctaattcta agcaaagcca
     4741 agatgagcct ttgagaagca gaaaagtgtt tgtggggcgc tgtacagagg acatgactga
     4801 ggatgagctg cgggagttct tctctcagta cggggatgtg atggatgtct tcatccccaa
     4861 gccattcagg gcctttgcct ttgttacatt tgcagatgat cagattgcgc agtctctttg
     4921 tggagaggac ttgatcatta aaggaatcag cgttcatata tccaatgccg aacctaagca
     4981 taatagcaac agacaactcg aacgaagcgg gcggagtggg ggcaaccccg gcggatctgg
     5041 taatcaaggc ggaagcggca atagccgggg tgggggagcc ggtctcggca ataatcaagg
     5101 atctaacatg ggcggcggta tgaactctgg cgcatccagt atcaacccgg ccatgatggc
     5161 agcagcacag gccgccctgc aaagttcatg gggaatgatg ggtatgttgg caagtcagca
     5221 gaaccagtca ggaccttccg ggaataatca aaaccaaggg aacatgcaac gcgaacccaa
     5281 tcaagcctca ggatcaggca ataatagcag ttccggctca aattccggcg cggcgatagg
     5341 gtctggctcc gcctcaaatg cgggaagcgg atcagggagt aacggaggat caggctcatc
     5401 tatggattca aaatccagcg gcagtggtat gtagacccag ctttcttgta caaagtggtt
     5461 gatcctctag agctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
     5521 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
     5581 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
     5641 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
     5701 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
     5761 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
     5821 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
     5881 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca
     5941 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
     6001 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc
     6061 tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc
     6121 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     6181 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     6241 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     6301 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     6361 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     6421 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     6481 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     6541 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     6601 tcccagtcat agctgtccct cttctcttat ggagatccct cgactgcatt aatgaatcgg
     6661 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6721 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6781 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6841 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6901 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6961 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7021 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7081 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7141 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7201 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7261 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7321 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7381 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7441 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7501 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7561 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7621 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7681 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7741 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7801 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7861 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7921 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7981 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8041 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8101 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8161 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8221 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     8281 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8341 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8401 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8461 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8521 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8581 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8641 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8701 tcactcgacg cggccagctt ggtcgagctg gatacttccc gtccgccagg gggacatgcc
     8761 ggcgatgctg aaggtcgcgc gcattcccga tgaagaggcc ggttaccgcc tgttgacctg
     8821 gtgggacggg cagggcgccg agttcataag agaagaggga cagctatgac tgggagtagt
     8881 caggagagga ggaaaaatct ggctagtaaa acatgtaagg aaaattttag ggatgttaaa
     8941 gaaaaaaata acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta
     9001 tggaataagg aatggacagc agggggctgt ttcatatact gatgacctct ttatagccaa
     9061 cctttgttca tggcagccag catatgggca tatgttgcca aactctaaac caaatactca
     9121 ttctgatgtt ttaaatgatt tgccctccca tatgtccttc cgagtgagag acacaaaaaa
     9181 ttccaacaca ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca
     9241 aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctca gtggtatttg
     9301 tgagccaggg cattggccac accagccacc accttctgat aggcagcctg cacctgagga
     9361 gtgaattctt tgccaaaatg atgagacagc acaacaacca gcacgttgcc caggagctgt
     9421 aggaaagaga agaaggcatg aacatggtta gcagaggggc ccggtttgga ctcagagtat
     9481 tttatcctca tctcaaaca
//
