LOCUS       sequence-26181-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Shumway SD, Li Y, Xiong Y
  TITLE     14-3-3beta binds to and negatively regulates the tuberous sclerosis 
            complex 2 (TSC2) tumor suppressor gene product, tuberin.
  JOURNAL   J Biol Chem. 2003 Jan 24. 278(4):2089-92.
  PUBMED    12468542
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Jan 24. 278(4):2089-92."
COMMENT     Sequence Label: pcDNA3-myc3-Tsc1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..113
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atggagcaaa agctcatctc agaggaagat ctggaattag
      121 agcaaaagct catctcagag gaagatctgg aattagagca aaagctcatc tcagaggaag
      181 atctggaatt caggatcctg atatccgcgg tacctatggc ccaacaagca aatgtcgggg
      241 agcttcttgc catgctggac tcccccatgc tgggtgtgcg ggacgacgtg acagctgtct
      301 ttaaagagaa cctcaattct gaccgtggcc ctatgcttgt aaacaccttg gtggattatt
      361 acctggaaac cagctctcag ccggcattgc acatcctgac caccttgcaa gagccacatg
      421 acaagcacct cttggacagg attaacgaat atgtgggcaa agccgccact cgtttatcca
      481 tcctctcgtt actgggtcat gtcataagac tgcagccatc ttggaagcat aagctctctc
      541 aagcacctct tttgccttct ttactaaaat gtctcaagat ggacactgac gtcgttgtcc
      601 tcacaacagg cgtcttggtg ttgataacca tgctaccaat gattccacag tctgggaaac
      661 agcatcttct tgatttcttt gacatttttg gccgtctgtc atcatggtgc ctgaagaaac
      721 caggccacgt ggcggaagtc tatctcgtcc atctccatgc cagtgtgtac gcactctttc
      781 atcgccttta tggaatgtac ccttgcaact tcgtctcctt tttgcgttct cattacagta
      841 tgaaagaaaa
//
