LOCUS       sequence-262064-        8665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector of RGM-eGFP with homologous arms around Sox2 
            super-enhancer DMR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Song Y, van den Berg PR, Markoulaki S, Soldner F, Dall'Agnese A, 
            Henninger JE, Drotar J, Rosenau N, Cohen MA, Young RA, Semrau S, 
            Stelzer Y, Jaenisch R
  TITLE     Dynamic Enhancer DNA Methylation as Basis for Transcriptional and 
            Cellular Heterogeneity of ESCs.
  JOURNAL   Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 
            10.1016/j.molcel.2019.06.045.
  PUBMED    31422875
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 
            10.1016/j.molcel.2019.06.045."
COMMENT     Sequence Label: Sox2-SE-RGM-eGFP targeting vector
FEATURES             Location/Qualifiers
     source          1..8665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(926..928,929..931,932..1645)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(926..928,929..931,932..1642)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(971..992)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1232..1251)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1579..1600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1735..1754)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1800..1855
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1854..1873)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    2216..2249
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2310..2809
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2345..2368)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2742..2761
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2849..3448
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2849..2868)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4005..4038
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4907..4926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4908..4926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4933..4950)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4933..4949)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4942..4964)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5090..5518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5177..5196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5387..5408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5862..6656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5916..5935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6526..6545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6674..6742,6743..7534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6892..6911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7705..8293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8194..8213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8447..8464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8581..8602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8617..8622,8623..8640,8641..8647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(8655..8665,1..6)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8660..8665,1..17)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg taccgagctc
       61 ggatccacta gtaacggccg ccagtgtgct ggaattcgcc cttttcctgc aggccggggt
      121 ttcctgatct cttgcccaag tggctgctgt gcccagcatg tgggcccaat ttattttcct
      181 actttttcca ggatgcgcag atgcaggaac tatctttggt gtttctgtct ttctgcctgc
      241 tcaccgcagt ttccgctaac aaaacaagcc tttgtgcact ttgtttgcag tgttgagaac
      301 tgatcacagg gacgtgggag aacccctccc tggagccgcg cgcggcgacc ctttctgtgg
      361 atctggctta ctgaggctca gtccgctttg ccacaccgcc tcctgctggc ggctagttga
      421 cctgccgcgc tttttcttct gtgcgtcaga tacttcacca ggcctgaggg tcttctcttt
      481 tcttttgaaa tagggctcaa ggaacccagg cagactgcaa actcactaag tggcccgaag
      541 ctgactttga actcatgatc ttcttgcatc cattttcgtg gagctggggt tgcccatgca
      601 taccaccaca cccagctttc cgagccagac aattgacgct caaatttccg cagtaggaat
      661 gctcaagcat tccttttggt agctgccttt tggcaggaca ttccggtcag agggacagag
      721 acccctgcat tgcggcaaaa atgtgcgcat gtgcagccat tgcctgggac gcatgcgtag
      781 ggagccgcgc gacaaacctg agccattgcg gcaagactag cgcagagagg agagggagcc
      841 ggagatgcca gacgcttggt tctgaggagt gatttgcaac gcaatggagc gaggaaggtc
      901 agctgggctt gtggattctg ctagcatggt gagcaagggc gaggagctgt tcaccggggt
      961 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1021 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1081 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1141 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1201 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1261 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1321 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1381 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1441 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1501 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1561 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1621 cggcatggac gagctgtaca agtaaacgcg tgaattcact cctcaggtgc aggctgccta
     1681 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     1741 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     1801 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     1861 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     1921 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     1981 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     2041 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     2101 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     2161 ccctcttctc ttatggagat cccttaatta acgatcatat tcaataaccc ttaatataac
     2221 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa
     2281 gcttaggatc tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     2341 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     2401 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     2461 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     2521 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     2581 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     2641 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     2701 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     2761 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca
     2821 tctagatctc gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     2881 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2941 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     3001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     3061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     3121 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     3181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     3241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     3301 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccacaacc tccccttcta
     3361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     3421 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     3481 aaggagcgca cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga
     3541 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
     3601 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
     3661 tggggtggga ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga
     3721 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
     3781 attcctccca ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct
     3841 tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg
     3901 aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa
     3961 gttctaattc catcagaagc tggtcgagat ccggaaccct taatataact tcgtataatg
     4021 tatgctatac gaagttatta ggtccctcga agaggttcac taggcgcgcc ggagggggct
     4081 gcattctcag acaactctca gtggctagct tgccctttga caaaactttg ctaggaattt
     4141 accaccccta ccccccctcc ccagactctc cctttgtagc tcctctcttc ccttctctct
     4201 cacttggctc cctgctcatt tgactgacag cttgggcaaa gactcctctc ctgctgagct
     4261 gagaccctga agagaattcc actaacacgt aaacaaggtc ttggctgacc ttgaactcac
     4321 tatgtagacc ctgcaggtct cctattctga cttctaaatt ccgggattaa aggcatcacc
     4381 acgcctagtc attgatatgt cttattattt attttatgag tatgaatatt ttgcctgttt
     4441 gtatgtctgt gcaccatgtg catgcctggt gctaatggag gccagaggag ggcatcaggc
     4501 cctctggagc tagagttaca gatggttgtg agcctctatg cacatgctgg gaccagaacc
     4561 caggtcctct gcaagagcag ccagtgctct taaccactga gccatctttt caactcctca
     4621 atttccatct taaattttat tttgtgctgg gctagccttg gctgtataat aagacccagt
     4681 ttcaataagc aataaggttc attttatgtg tgtcttagag gcttgtatat cacactgctt
     4741 aatcttttat tttggtgggg tacaggacat tgaaattgag agagggtttc ttgtaactgc
     4801 agctggtctc gaacctgttt cgtagcggcc ggccaataag ggcgaattct gcagatatcc
     4861 atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct atagtgagtc
     4921 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4981 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5041 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     5101 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5161 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5221 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5281 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5341 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5401 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5461 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5521 aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc
     5581 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
     5641 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
     5701 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     5761 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
     5821 gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5881 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5941 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6001 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6061 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6121 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatcccacc
     6181 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6241 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6301 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6361 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6421 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6481 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6541 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6601 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattg
     6661 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6781 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6841 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6901 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7021 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7081 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7201 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7261 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7501 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7561 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7621 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7681 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7741 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7801 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7861 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7921 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7981 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8041 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8101 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8161 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8281 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8341 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8401 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8461 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     8521 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     8581 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8641 tatgttgtgt ggaattgtga gcgga
//
