LOCUS       sequence-262074-        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rab6A fused to the red fluorescent protein Ruby3 in N-ter under 
            control of a weak L30 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Deffieu MS, Cesonyte I, Delalande F, Boncompain G, Dorobantu C, Song
            E, Lucansky V, Hirschler A, Cianferani S, Perez F, Carapito C, 
            Gaudin R
  TITLE     Rab7-harboring vesicles are carriers of the transferrin receptor 
            through the biosynthetic secretory pathway.
  JOURNAL   Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7803. doi: 
            10.1126/sciadv.aba7803. Print 2021 Jan.
  PUBMED    33523982
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-01-8"; volume: "7(2). pii: 7/2/eaba7803. doi"; pages: " 
            10.1126/sciadv.aba7803. Print 2021 Jan"
COMMENT     Sequence Label: pBS L30 Ruby3-Rab6A
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     protein_bind    745..769
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
       61 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcag accgtacgag
      121 ggagtgcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
      181 attcttgcca cgtcgttcat gtatggcagc cgtaccttta tcaagtaccc ggccgacatc
      241 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
      301 gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg cgagctcgtc
      361 tacaacgtca aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag
      421 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagagga
      481 tacactgaca tcgcactgaa agttgatggt ggtggccatc tgcactgcaa cttcgtgaca
      541 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtgtcca tgccgttgat
      601 caccgcctgg aaaggatcga ggagagtgac aatgaaacct acgtagtgca aagagaagtg
      661 gcagttgcca aatacagcaa ccttggtggt ggcatggacg agctgtacaa gggtggttcc
      721 ggtggatctg gaggttctga tatcacaagt ttgtacaaaa aagcaggctt ctccacgggc
      781 ggagacttcg ggaatccgct gaggaaattc aagctggtgt tcctggggga gcaaagcgtt
      841 ggaaagacat ctttgatcac cagattcatg tatgacagtt ttgacaacac ctatcaggca
      901 acaattggca ttgacttttt atcaaaaact atgtacttgg aggatcgaac aatcaggctt
      961 cagctgtggg atactgcggg tcaggaacgt ttccgtagcc tcattcccag ttacatccgt
     1021 gattctgctg cagctgtagt agtttacgat atcacaaatg ttaactcatt ccagcaaact
     1081 acaaagtgga ttgatgatgt cagaacagaa agaggaagtg atgttatcat catgctagta
     1141 ggaaataaaa cagatcttgc tgacaagagg caagtgtcaa ttgaggaggg agagaggaaa
     1201 gccaaagagc tgaatgttat gtttattgaa actagtgcaa aagctggata caatgtaaag
     1261 cagctctttc gacgtgtagc agcagctttg ccgggaatgg aaagcacaca ggacagaagc
     1321 agagaagata tgattgacat aaaactggaa aagcctcagg agcaaccagt cagtgaagga
     1381 ggctgttcct gctaa
//
