LOCUS       sequence-262085-        9075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]
FEATURES             Location/Qualifiers
     source          1..9075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    83..138
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(536..553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(707..1295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(787..806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1466..2257,2258..2326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2089..2108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2327..2431)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2499..2517
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    2992..3047
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     intron          3244..3816
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4162..4181)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4431..4449
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      6071..6080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        6233..6536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6537..6740
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6691..6711
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6802..6811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6808..7509
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     protein_bind    complement(8310..8334)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          8389..8961
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
       61 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
      121 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
      181 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
      241 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
      301 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
      361 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
      421 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga ctgcattaat
      481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     2101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     2461 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     2521 ttcgtcttca ctcgacgcgg ccagcttggt cgagctggat acttcccgtc cgccaggggg
     2581 acatgccggc gatgctgaag gtcgcgcgca ttcccgatga agaggccggt taccgcctgt
     2641 tgacctggtg ggacgggcag ggcgccgagt tcataagaga agagggacag ctatgactgg
     2701 gagtagtcag gagaggagga aaaatctggc tagtaaaaca tgtaaggaaa attttaggga
     2761 tgttaaagaa aaaaataaca caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc
     2821 ttttctatgg aataaggaat ggacagcagg gggctgtttc atatactgat gacctcttta
     2881 tagccaacct ttgttcatgg cagccagcat atgggcatat gttgccaaac tctaaaccaa
     2941 atactcattc tgatgtttta aatgatttgc cctcccatat gtccttccga gtgagagaca
     3001 caaaaaattc caacacacta ttgcaatgaa aataaatttc ctttattagc cagaagtcag
     3061 atgctcaagg ggcttcatga tgtccccata atttttggca gagggaaaaa gatctcagtg
     3121 gtatttgtga gccagggcat tggccacacc agccaccacc ttctgatagg cagcctgcac
     3181 ctgaggagtg aattctttgc caaaatgatg agacagcaca acaaccagca cgttgcccag
     3241 gagctgtagg aaagagaaga aggcatgaac atggttagca gaggggcccg gtttggactc
     3301 agagtatttt atcctcatct caaacagtgt atatcattgt aaccataaag agaaaggcag
     3361 gatgatgacc agggtgtagt tgtttctacc aataagaata tttccacgcc agccagaatt
     3421 tatatgcaga aatattctac cttatcattt aattataaca attgttctct aaaactgtgc
     3481 tgaagtacaa tataatatac cctgattgcc ttgaaaaaaa agtgattaga gaaagtactt
     3541 acaatctgac aaataaacaa aagtgaattt aaaaattcgt tacaaatgca agctaaagtt
     3601 taacgaaaaa gttacagaaa atgaaaagaa aataagagga gacaatggtt gtcaacagag
     3661 tagaaagtga aagaaacaaa attatcatga gggtccatgg tgatacaagg gacatcttcc
     3721 cattctaaac aacaccctga aaactttgcc ccctccatat aacatgaatt ttacaatagc
     3781 gaaaaagaaa gaacaatcaa gggtccccaa actcaccctg aagttctcag tctagagtcg
     3841 agaggatcaa tcactttgta caagaaagct gggtctagat agcttacttg tacagctcgt
     3901 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
     3961 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
     4021 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
     4081 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
     4141 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
     4201 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
     4261 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     4321 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
     4381 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
     4441 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctg aggaaaaaaa aggaaagggc
     4501 atgctcgcca tgtgcaagat acagtgttga atgtggtgca aaatataaag atgtggaaga
     4561 aaaagccact gaaaataata tgaggaaata aaaaactaga gtaaattatc agtggaacaa
     4621 tttctattat acaagacact acacccatac attctcctaa tgctcatgta agaatttctc
     4681 caaaaatacc ttccagcact acaaactaga aaaaaaaaga gacatggaca ccaaattaag
     4741 ttcttaatag tgaagaacaa aagaactacc ttgcctgctc cagtggatcc agcaaccgcc
     4801 aacaactgtc ctctttctat cttgaaatta atatctttca ggacaggagt accaagaagt
     4861 gagaaattac tgaagaagag gctgtcatca ccattagaag tttttctatt gttattgttt
     4921 tgttttgctt tctcaaataa ttccccaaat ccctgttaaa aaaaaacaca cacacacaca
     4981 cacacacaca catcaaaaat aaaagatgag tttgtcagat attttcaata gatgcttgtt
     5041 ttatgtacca tactcacctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
     5101 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg
     5161 cccttgctgg ttccggtgcc tccactttca tcttcacctt tagtgatacc ggctgccgtc
     5221 acgaactcct tcaggaccat gtgatcgcgc ttctcgttcg ggtctttgct cagcgtggtc
     5281 tgggtgctca gatagtggtt gtccggcagg cgcaccgggc cgtcgccgat cggggtgttc
     5341 tgctgtttgt ggtcggcttc ctgctggctg ccgtcctcaa cgttgtggcg gatcttgaag
     5401 ttagccttga tgccgttctt ctgcttgtcg gcttcgatac ggacgttgtg gctgttgtgg
     5461 ttgtactcca gcttgtgacc caggatgttg ccgtcctcct tgaagtcgtt gcccttcagc
     5521 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct ccgcgcgggt cttgtaggtg
     5581 ccgtcgtcct tgaactcgat ggtgcgctcc tggacgtagc cttccggcat ggcggacttg
     5641 aagaagtcgt gacgcttcat gtggtccggg tagcggctga agcactgcac gccgtaggtc
     5701 agggtggtca cgagggtcgg ccacggcacc ggcagcttgc cggtggtgca gatgaacttc
     5761 agggtcagct tgccctcggt ggcatcgccc tcgccctcgc cacgcacgct gaacttgtgg
     5821 ccgtttacgt cgccgtccag ctcgactttg ataggcacag tacctgtaaa tagttcctct
     5881 cctttactgg taccatcatc agggaatgag cttacatttc tcctcttcag catctggtca
     5941 tccttcttag ctttcctcag ctccacattg acctctattc tgcgacgcct catttctgta
     6001 ctgtcttttc ccttgttctt gaatctgtga agacgggcag ctggtgtatt agcattctcg
     6061 ttggtggatc ccatggtggc accggtgaat tctccaggcg atctgacggt tcactaaacg
     6121 agctctgctt atataggcct cccaccgtac acgccacctc gacatactcg agtagttatt
     6181 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     6241 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6301 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6361 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6421 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6481 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     6541 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     6601 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     6661 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     6721 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctaggga tcctctagtc
     6781 agctgacgcg ttaccaccgg tgccaccatg ggaagcgagc tgattaagga gaacatgcac
     6841 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
     6901 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     6961 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
     7021 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
     7081 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     7141 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
     7201 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
     7261 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
     7321 atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
     7381 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
     7441 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
     7501 aagcttaatt ccggaatgtc tgaatatatt cgggtaaccg aagatgagaa cgatgagccc
     7561 attgaaatac catcggaaga cgatgggacg gtgctgctct ccacggttac agcccagttt
     7621 ccaggggcgt gtgggcttcg ctacaggaat ccagtgtctc agtgtatgag aggtgtccgg
     7681 ctggtagaag gaattctgca tgccccagat gctggctggg gaaatctggt gtatgttgtc
     7741 aactatccaa aagataacaa aagaaaaatg gatgagacag atgcttcatc agcagtgaaa
     7801 gtgaaaagag cagtccagaa aacatccgat ttaatagtgt tgggtctccc atggaaaaca
     7861 accgaacagg acctgaaaga gtattttagt acctttggag aagttcttat ggtgcaggtc
     7921 aagaaagatc ttaagactgg tcattcaaag gggtttggct ttgttcgttt tacggaatat
     7981 gaaacacaag tgaaagtaat gtcacagcga catatgatag atggacgatg gtgtgactgc
     8041 aaacttccta attctaagca aagccaagat gagcctttga gaagcagaaa agtgtttgtg
     8101 gggcgctgta cagaggacat gactgaggat gagctgcggg agttcttctc tcagtacggg
     8161 gatgtgatgg atgtcttcat ccccaagcca ttcagggcct ttgcctttgt tacatttgca
     8221 gatgatcaga ttgcgcagtc tctttgtgga gaggacttga tcattaaagg aatcagcgtt
     8281 catatatcca atgccgaacc ttagactaga cccagctttc ttgtacaaag tggttgatcc
     8341 tctactagta agcttgcatg cgatatctct agagctgaga acttcagggt gagtttgggg
     8401 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
     8461 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
     8521 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
     8581 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     8641 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
     8701 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
     8761 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
     8821 aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
     8881 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
     8941 gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
     9001 tttggcaaag aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     9061 gccaatgccc tggct
//
