LOCUS       sequence-26215-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pnlacZ-ACN
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             231..251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggnccctgt tgtgctgcaa aggagagact gggagaaccc tggagtgacc cagctcaaca
       61 gactggctgc ccaccctccc tttgcctctt ggaggaactc tgaggaagcc aggacagaca
      121 ggcccagcca gcagctcagg tctctcaatg gagagtggag gtttgcctgg ttccctgccc
      181 ctgaagctgt gcctgagtct tggctggagt gtgacctccc agaggcagtt cccaagaaga
      241 agaggaaagt tgaggctgac actgttgtgg tgccaagcaa ctggcagatg catggctatg
      301 atgcccccat ctacaccaat gtcacctacc ccatcactgt gaacccccct tttgtgccca
      361 ctgagaaccc cactggctgc tacagcctga ccttcaatgt tgatgagagc tggctgcaag
      421 aaggccagac caggatcatc tttgatggag tcaactctgc cttccacctc tggtgcaatg
      481 gcaggtgggt tggctatggc caagacagca ggctgccctc tgagtttgac ctctctgcct
      541 tcctcagagc tggagagaac aggctggctg tcatggtgct caggtggtct gatggcagct
      601 acctggaaga ccaagacatg tggaggatgt ctggcatctt cagggatgtg agcctgctgc
      661 acaagcccac cacccagatt tctgacttcc atgttgccac caggttcaat gatgacttca
      721 gcagagctgt gctggaggct gaggtgcaga tgtgtggaga actcagagac tacctgagag
      781 tcacagtgag cctctggcaa ggtgagaccc angtggcctc tggcacagcc ccctttggag
      841 gagagatcat tgatgagaga ggaggct
//
