LOCUS       sequence-26228-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Hu H, Clarkson MW, Hermans J, Lee AL
  TITLE     Increased rigidity of eglin c at acidic pH: evidence from NMR spin 
            relaxation and MD simulations.
  JOURNAL   Biochemistry. 2003 Dec 2. 42(47):13856-68.
  PUBMED    14636053
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2003 Dec 2. 42(47):13856-68."
COMMENT     Sequence Label: pET21a- hPTP1e-PDZ2
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             373..390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(443..461)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      457..504
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(629..648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcctaa gcctggagat atctttgagg
       61 ttgaactggc taaaaatgat aacagcttgg ggataagtgt cacgggaggt gtgaatacga
      121 gtgtcagaca tggtggcatt tatgtgaaag ctgttattcc ccagggagca gcagagtctg
      181 atggtagaat tcacaaaggt gatcgcgtcc tagctgtcaa tggagttagt ctagaaggag
      241 ccacccataa gcaagctgtg gaaacactga gaaatacagg acaggtggtt catctgttat
      301 tagaaaaggg acaatctcca acataaggat ccgaattcga gctccgtcga caagcttgcg
      361 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      421 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      481 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      541 acgcgccctg tagcggcgca ttaaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      661 acgttcgccg gctttccccg tcaagctct
//
