LOCUS       sequence-26229-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Hu H, Clarkson MW, Hermans J, Lee AL
  TITLE     Increased rigidity of eglin c at acidic pH: evidence from NMR spin 
            relaxation and MD simulations.
  JOURNAL   Biochemistry. 2003 Dec 2. 42(47):13856-68.
  PUBMED    14636053
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2003 Dec 2. 42(47):13856-68."
COMMENT     Sequence Label: pET28a-EgC*
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    466..490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(490..509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(491..509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(593..612)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cnttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgacggagct
       61 cgaattcgcc acacgcgaac tttacggtca tcgcgtggtg cacaactgat cacggtttga
      121 taatcaatcg cgcagggcag aacgaaaccg tcgttgtagt cggtgtgttc accaatcaaa
      181 ttcacgcggc caggcgatta attaattact aacctacgtg aggaacatga ttaacaacgt
      241 tagtacctgg gttgtagaaa acgcgtacac ggttgtaacg aaggtccaga gtaacaggag
      301 aaccttccgg caggaagtag acgtcgtatt gtgggtaatg cagagtgaag tattcacgag
      361 cctggtcgac agttttacca acaacctcgg gccaagactt aagttcagaa ccaaattcag
      421 tcatggtata tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      481 tcacaattcc cctatagtga gtcgtattaa tttcgtggga tcgagatctc gatcctctac
      541 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      601 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      661 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt ggggngccat ctccttgcat
      721 gcnnnattcc ttgcggcggc ggtgctcaac ggnctcaacc tactactggg ctgcttccta
      781 angcngnant cgna
//
