LOCUS       sequence-262314-       11190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Leishmania donovani ectodomain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Roberts AJ, Ong HB, Clare S, Brandt C, Harcourt K, Franssen SU, 
            Cotton JA, Muller-Sienerth N, Wright GJ
  TITLE     Systematic identification of genes encoding cell surface and 
            secreted proteins that are essential for in vitro growth and 
            infection in Leishmania donovani.
  JOURNAL   PLoS Pathog. 2022 Feb 24;18(2):e1010364. doi: 
            10.1371/journal.ppat.1010364. eCollection 2022 Feb.
  PUBMED    35202447
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2022-02-24"; volume: "18(2):e1010364. doi"; pages: 
            " 10.1371/journal.ppat.1010364. eCollection 2022 Feb"
COMMENT     Sequence Label: D19_Ld_130940
FEATURES             Location/Qualifiers
     source          1..11190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6354..6371
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6423..6478
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6477..6496)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8714..8732)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8800..8904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8905..8973,8974..9765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9123..9142)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9936..10524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10425..10444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10678..10695
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10812..10833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10848..10853,10854..10871,10872..10878)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10886..10902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10891..10913
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10910..10926
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10910..10926
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 ccgaaggacc ttggcctctg tctgtgcagg ccgcccacag atccaccgat gcccacgccg
     1261 agggccccag catccacccc agaaagtact acctggggaa gtacggcgcc cactgcatcc
     1321 ggaacctgac cgagttcgtg gccagcgagg acgccgccga gagactggcc tgtttctctg
     1381 gcagaggcgt gcgggtggcc ctgctggaaa caggcctgtg tgccggaatc gccgagcgga
     1441 gcagaaacag cgtgacctgc accagcgtgg tgcccggcgt ggcctgtgaa gatgccggct
     1501 gtgcccacgg cacccgctct gtgtcagtgc tggccgggca gctgtccgtg tccgcccctc
     1561 tgcctagaga gcccctggaa gagacagcct ggcggaacag agagacacac ctggccctgg
     1621 ccgaccagta catcggcctg gcccctgaca gcaccgtccg ggtgctgcgg atcttcgaca
     1681 gacagggccg gaccaaccgg cggcatctgg ccagagcact ggacctgctg ctgagagagg
     1741 ccgaggacgg cgaagccggc agactgcaca gcgcctggaa taccagcagc gcccacatcc
     1801 ggcggaggga cgagacagtg gacgtgatca gcctgagcta cggcagcaag gactaccaca
     1861 gcagccccca ggtgcaggac cggctgtacc ggctgatgca cgagcacggc gtgatcgtgg
     1921 tggccgctgc cggcaacgat ggcgccagat tcggatctgt gcggagccct gccgacatgc
     1981 caggcgtgct ggctgtgggc gccctgagaa tggaatggca cggcagaagc gcccagacca
     2041 cccaggccag cagaaccctg ggcgatctgg cctctagcct ggctgctggc ggcggacaca
     2101 agagcgtggc ccacttcagc ggcaggggcc ctaccacctg ggagctgcct tttggcgccg
     2161 gaagggccaa gcccgatctg gtggctctgg gacagcacgt gtgggccgtg cagggggtgt
     2221 cagccgccgc tttttctgct gccgccccta gagccagatc cgcctctccc gcactgcagc
     2281 tgagatctgc cagcggcacc tctatcgccg ctcccatcgt ggctggggtg gtggcactgt
     2341 gcctggaagc cgcctggtcc agcacatctg ccgtcgccct ggctcagaac atgacaggcg
     2401 gaggcagctt cctggacgtg cggcagaaca ggctggcccg ggtgtccgac tccctgagag
     2461 tgcgggaagt gatcctgcgg accgccgtgc ctctgggcga agctgctgct agcctgcctc
     2521 cttggcccca ctctgccccc accacccccc tgcactctga tagcagcagc accgcctccg
     2581 aggcccaccc tagaagagtc ctggctggcg tgcccctgct gaagctgtac gccggctacc
     2641 tgagactgag cagagtgtcc atcctgtctc agggcgctgg cgaggtgcag cctctgaggg
     2701 ccctgcacgc cattgtggcc aaggcagccg ccagctccgc catcgatgcc cctgaggccc
     2761 tgaggaactg cgcctccttc gccatcccta cctgcgtgcg gatcggctac cggatcccca
     2821 gatgcagcaa cagcagctcc agctctagct cctccagcgg cgacatgagc accccccaga
     2881 gctgccagcc cccccacgat cctgatggca aggcccatgg cgatagcccc ctgcccggca
     2941 gatccggcat gagcctggat gccccaccag ccgcctactg gtggccattc agcgaccagg
     3001 ccgtgtaccc tggcgccacc cccgtgctgc tcaacatctc cctgcacctg tgccccccta
     3061 gctctcctgc cgccgcagac cagggaacca gagatgccgc tgcagccgct tctggcaagg
     3121 gggccagaag aggcagcacc acacacatcc acgtgaccca tgtgatcacc aaggtgtccg
     3181 gccacgtgcg ggccagagaa ggccacccct ccagaaactc cagccaccag tgcgacgcct
     3241 gccctctgct gttcgagggc gccaagagca gacacagagc cggctccacc tcccccaccc
     3301 ggctgctgaa tctgacagct cggagcgcct ccccacgggc ctcttgctct gatggcgaag
     3361 tggccggcga aggcgccaca gtgatgggcc ctagacacac acagactgcc gtgtgcagca
     3421 gcgtgaagcg gtggtccacc cagcagagat ggattcacca tctgctgagg gtggccaccg
     3481 agctgacagt ggtggctagc agaagccaga cccgggccaa cgtggtggaa cagctgagcc
     3541 ccccacctac cagcttcagc ctgtcagtgg ccgtgtctag ccccgccagc gctcacaccc
     3601 acctgtgtta cgatgtggcc agggaaaccg tggcagtgtg gcggaagaag ggcaagtcca
     3661 gccccgcttc cgagaaggat gccgcagctg gcagacgcag cgccgatggc gacgacggcc
     3721 acgacaagca gcatagcagg gacggcgctc aggtgcccgc cgaccacgat agatgcgtgc
     3781 cactgttcca cctgttcaga gccctgagcg tggaaggcgc tctgcacatc ttcaccggct
     3841 cctctagtag ccagcccgcc ctgaccgtgc ccttcgccgt cagagtggtc gagccccctc
     3901 caagagtgca gagagtgctg atcgacacca gcctggactg tttcaaccca accaccgcca
     3961 ccagcaacct gtttatcgcc ggcgacgatc cccacgagag cgacgctggg ggagctggcg
     4021 ctgctctgcg gagaggcaga cagagacaga ggcacgagcg ggcctatgcc gaggcctctg
     4081 gcggagatca ccccttcacc aacctggcac tgctgtacct gtatctgcgg cacacactgg
     4141 gcatggccgt ggaaaccttc ccactgctgc acatgtccac aatcgccacc tccattgccg
     4201 ctagccacag cccacctctg tggcccccag ccgagaggac cagcaatgtg tccaggaccg
     4261 cccagagggc tcccgccgga acactggccc acattggcac cctgatcatc gtggaccccg
     4321 agcgccccct gaccagaggc atgagaaggc tgctcaccag agccgtgctg ggaggcggag
     4381 ccgatagaag cgctgacggc ctgaatgtgc tgctggtcac cgactggtac tctgccgacc
     4441 tggccgctca gctgcactgg gctcgggacg agtccctgta cgacgccgaa cggttcagcc
     4501 acatcaacgg caccgacgcc acagacgagg tggcaggcgc cgacggggat agggatgccg
     4561 tgcgggatct gcgggctctg aggcagagcg agaacggctc tacacggggc ctggcaggca
     4621 gctctcacgt gcccagctgg aaccggtggc tgagcgaagt gactgtggcc tccggctccg
     4681 ccgccaacag ctccgattgt gatggcgcag ctcccctggg cagcgaggga ctgctggatg
     4741 gaaccagcga actgcctttc gagctgtctg agagcatcgt gatcgacggg gctgtgctgg
     4801 tggacgcagc cgcacccaga agaagaagcg agcccgaggg caacgccttt gccagccacg
     4861 gcggaaccgt gacaagcgcc aaggtggtcg ctctgagaag cctgggccag ctgtgtgctg
     4921 ccggggtgct gagatggcgg ctccctgctc aggctgctgt gcagaggcag gccggcaggg
     4981 ctacagccgt gggagctagc aagggcgtcc gcgagcccaa tgtgtacccc tccggcaatg
     5041 gcacctgtag cacaggcgac gccagcaaca cctctgccat gcccagcacc caggaaggcg
     5101 aggccgtcat ctgcaacgtg atgccctcct gggcccagca gcagcggcgg ctggtcaaga
     5161 gaaccgtggt gtccacccgg gacggatccg caccagctgc cgtggtcgaa gatggatggg
     5221 aggacgtgcc cgtggctaac ggcgacggca ggctcgtcag aatcggcgga ctggaagctg
     5281 ccgaagtgtc taccatggct cctgctgcca caggcgcctc tgacgccacc cagagcctga
     5341 cacacggcgt cctgggcttt ctgaccctgc cacctagctc caccctggtg tcaaccgtgt
     5401 ctgccaaccg gttccggcca ggccggatcg ccatctttac cgacagcgat tgcctgtctg
     5461 cctccgacca ccacgcccag gccgctctgg atgaactcga agccctgctc taccctccca
     5521 gcgccagccc ttccctgcct gtgaccacat gcgccacatg ggattgcttc agcaggaccc
     5581 cagacggaca gcggctgctc caggccgagt ctgcccagtc cagcgtgtgt gtggaagtcg
     5641 tgaaagaact gctcctgtgg ctgcacaccg gcaatctgca tcggtggcgg gacagcgcac
     5701 agctgcagtg tgaagcccgg acctgggcta gagcccggca cagaagcaca gccgacacca
     5761 caccaggggc cggaagcgtg ctcgagccta aagccggcga ggactctgtg gaaggggaca
     5821 gagaaaccgc caacttcgac gagctgctgg gcacagcccc cgagagggcc catgtgggag
     5881 aagtcgtgtg gcgcctgtgg gccagcctgg tggaaagcac cgagcgcgac gccgcttcct
     5941 ttctggtgga tggccccaaa gagggcaccc ggttccaggg cgattacgcc gcccatcagg
     6001 aaagcgccgc agccaacgtc atgcgggccc tgatggtcaa ataccacggc gattggagag
     6061 cccatctcct ggatcacaac gtgtcccccc caagcactgg cgccgctggg gattcctaca
     6121 gccagtccct gcggcctgcc aggggcagaa gatctggcat tgccaccgtc cagcagcctg
     6181 aacagcacat tagcctgatc gagagcctgc tccccctgtg cggcatgctg aacgctctca
     6241 gatggctgca gcatcctggc gcgcccggca gcctgcacca catcctggac gcccagaaga
     6301 tgctgtggaa ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc
     6361 atcaccacca ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     6421 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     6481 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     6541 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     6601 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     6661 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     6721 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     6781 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     6841 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     6901 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     6961 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     7021 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     7081 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     7141 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     7201 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     7261 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     7321 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     7381 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     7441 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     7501 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     7561 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     7621 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     7681 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     7741 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     7801 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     7861 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     7921 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     7981 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     8041 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     8101 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     8161 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     8221 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     8281 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     8341 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     8401 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     8461 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     8521 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     8581 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     8641 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     8701 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     8761 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8821 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8881 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8941 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9001 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9061 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9121 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     9181 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9241 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9301 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9361 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9421 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     9481 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9541 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9601 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9661 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9721 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9781 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9841 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9901 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9961 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10021 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10081 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10141 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10201 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10261 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10321 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10381 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10441 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10501 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10561 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10621 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10681 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10741 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10801 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10861 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10921 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
    10981 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
    11041 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
    11101 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
    11161 tttatattgg ctcatgtcca atatgaccgc
//
