LOCUS       sequence-26239-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag ADPGK
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..80
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttctctag gcgccggccg gatccagaat tcgccaccat ggggtcttca aaatctaaac
       61 caaaggaccc cagccagcgc cggcgcagga tccgaggtta ccttgactac aaagacgatg
      121 acgacaagca attgacaagt ttgtacaaaa aagttggcat ggcgctgtgg cgcggctccg
      181 cgtacgcggg cttcctggcg ctggccgtgg gctgcgtctt cctgctggag ccagagctgc
      241 caggctcggc gctgcgctct ctctggagct cgctgtgtct ggggcccgcg cctgcgcccc
      301 cgggacccgt ctcccccgag ggccggttgg cggcagcctg ggacgcgctt atcgtgcggc
      361 cagtccggcg ctggcgccgc gtggcagtgg gagtcaatgc atgtgttgat gtggtgctct
      421 caggggtgaa gctcttgcag gcacttggcc ttagtcctgg gaatgggaaa gatcacagca
      481 ttctgcattc aaggaatgat ctggaagaag ccttcattca cttcatgggg aagggagcag
      541 ctgctgagcg cttcttcagt gataaggaaa cttttcacga cattgcccag gttgcgtcag
      601 agttcccagg agcccagcac tatgtaggag gaaatgcagc tttaattgg
//
