LOCUS       sequence-262409-       14578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14578)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 14578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow3
FEATURES             Location/Qualifiers
     source          1..14578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4382..4415
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    4424..4457
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    4466..4499
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      4506..4515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4518..4538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4539..5243
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    5426..5547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5562..5595
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     regulatory      5603..5612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5924..6631
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    6831..6952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6967..7000
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      7008..7017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7329..7331,7332..7334,7335..8045)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7329..7331,7332..7334,7335..8045)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7374..7395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7635..7654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7982..8003
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8245..8366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8385..8418
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      8426..8435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8747..9442
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    9642..9763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12555..12574)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12674..12696
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12734..12752)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12820..12924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12925..12993,12994..13785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13143..13162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13956..14544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14445..14464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
       61 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      121 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      181 gttggccgat tcattaatgc aggatcgcgg ccgcgatccc cgggcgagct cgaattcaga
      241 gaccgggaac caaactagcc tttaaaaaac ataagtacag gagccagcaa gatggctcag
      301 tgggtaaagg tgcctaccag caagcctgac agcctgagtt cagtccccac gaactacgtg
      361 gtaggagagg accaaccaac tctggaaatc tgttctgcaa acacatgctc acacacacac
      421 acacaaatag tataaacaat tttaaatttc atttaaaaat aatttgtaaa caaaatcatt
      481 agcacaggtt ttagaaagag cctcttggtg acatcaagtt gatgctgtag atggggtatc
      541 attcctgagg acccaaaacc gggtctcagc ctttccccat tctgagagtt ctctcttttc
      601 tcagccacta gctgaagagt agagtggctc agcactgggc tcttgagttc ccaagtccta
      661 caactggtca gcctgactac taaccagcca tgaagaaaca aggagtggat gggctgagtc
      721 tgctgggatg ggagtggagt tagtaagtgg ccatggatgt aatgacccca gcaatgctgg
      781 ctagaaggca tgcctccttt ccttgtctgg agacggaacg ggagggatca tcttgtactc
      841 acagaaggga gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt
      901 ggtgcatgct tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca
      961 agaccagcct gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg
     1021 gtggaaaatc tgaaagaaag agagaaagaa agaaagaaag aaaggaagaa agaaagaaag
     1081 agtggcaggc aggcaggcag gaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg
     1141 aaggaaaata ggtgcgactt caagatccgg agttacaagc agaatgcact gtttccctaa
     1201 cagggccaag tgttttgagt aactgaaggt gggcatgatg cctgggaagc agaaacaagc
     1261 caggcagatg caccccttgc cttgcttccg aagggctgca gtagcatgga aaacatggaa
     1321 aacaaccaat ccattccctt tgctgatata acaggctcca aagccaaaac ctgtcactgg
     1381 aggctcaaga gcagatctcc agccaagagg caaaggaatg ggggaagctg gagggcctcc
     1441 ctctggttat ccaggcttct gaaggttcaa gcaaagaaag ggttacaacc ttaaaaggag
     1501 agcgtcccgg ggtatgggta gaagactgct ccaccccgac ccccagggtc cctaaccgtc
     1561 ttttccctgg gcgagtcagc ccaatcacag gactgagagt gcctctttag tagcagcaag
     1621 ccacttcgga cacccaaatg gaacacctcc agtcagccct cgccgaccac cccaccccct
     1681 ccatcctttt ccctcagcct ccgattggct gaatctagag tccctccctg ctcccccctc
     1741 tctccccacc cctggtgaaa actgcgggct tcagcgctgg gtgcagcaac tggaggcgtt
     1801 ggcgcaccag gaggaggctg cagctagggg agtccaggtg agagcaggcc gacgggaggg
     1861 acccgcacat gcaaggaccg ccgcagggcg aggatgcaag ccttccccag ctacagtttt
     1921 gggaaaggat accagggcgc tcctatatgg gggcgcggga actggggaaa gaaggtgctc
     1981 ccaggtcgag gtgggagagg aaggcagtgc ggggtcacgg gctttctccc tgctaacgga
     2041 cgctttcgaa gagtgggtgc cggaggagaa ccatgaggaa ggacatcaag gacagccttt
     2101 ggtccccaag ctcaaatcgc tttagtggtg cgaatagagg gaggaggtgg gtggcaaact
     2161 ggagggagtc cccagcgggt gacctcgtgg ctggctgggt gcggggcacc gcaggtaaga
     2221 aaaccgcaat gttgcgggag gggactgggt ggcaggcgcg ggggagggga aagctagaaa
     2281 ggatgcgagg gagcggaggg gggagggagc gggagaatct caactggtag aggaagatta
     2341 aaatgaggaa atagcatcag ggtggggtta gccaagccgg gcctcaggga aagggcgcaa
     2401 agtttgtctg ggtgtgggct taggtgggct gggtatgaga ttcggggcgc cgaaaacact
     2461 gctgcgcctc tgccaaatca cgctacccct gtatctagtt ctgccaggct tctccagccc
     2521 cagccccaat tcttttctct agtgttcccc cttccctccc ctgaatctca agcccacact
     2581 ccctcctcca taacccactg ttatcaaatc taagtcattt gccacccaac aaccatcagg
     2641 aggcggaagc agacgggagg agtttgagat caacttgggc tacatcacga gttccaggct
     2701 caccaaggct tcttaaggag accttgtctc taaaattaat taattaatta attaatagtc
     2761 ccctttctct gccacagaac cttgggatct ggctcctggt cgcagctccc cccaccccag
     2821 gctgacattc actgccatag cccatccgga aatcctagtc tatttcccca tggatcttga
     2881 actgcagaga gaatggcaga gtggcccgcc ctgtgcaaag gatgttccta gcctaggtgg
     2941 agctcgcgaa ctcgcagact gtgcctctct tgggcaagga caggctagac agcctgccgg
     3001 tgtgttgagc tagggcactg tggggaaggc agagaacctg tgcagggcag caatgaacac
     3061 aggaccagaa aactgcagcc ctaggaacac tcaagagctg gccatttgca agcatctctg
     3121 gcctccgtgc ttctcactca tgtcccatgt cttatacagg cctctgtggc acctcgcttg
     3181 cctgatctca tccctagccg ttaagctttc tgcatgactt atcacttggg gcataatgct
     3241 ggatacctac cattttctta gaccccatca aaatcctatt tgagtgtacg gttcggagaa
     3301 cctcatttat ccggtaaatg tcttttactc tgctctcagg gagctgaggc aggacatcct
     3361 gagatacatt gggagaggag atacagtttc aataaaataa taggttgggt ggaggtacat
     3421 gcctataatg ccaccactca ggaaatggtg gcagcttcgt gagtttgagg ccaacccaag
     3481 aaacatagtg aaaccctgtc agtaaataag taagcaagta tttgagtatc tactatatgc
     3541 tagggctgac ctggacatta ggggtcatct tctgaacaaa ctagtgcttg agggaggtat
     3601 ttggggtttt tgtttgttta atggatctga atgagttcca gagactggct acacagcgat
     3661 atgactgagc ttaacacccc taaagcatac agtcagacca attagacaat aaaaggtatg
     3721 tatagcttac caaataaaaa aattgtattt tcaagagagt gtctgtctgt gtagccctgg
     3781 ctgttcttga actcactctg tagaccaggc tggcctggaa atccatctgc ctgcctctgc
     3841 ctctctgcct ctctgcctct ctgcctctct ctctgcctct ctctgcctct ctctgcccct
     3901 ctctgcccct ctctgcccct ctctgccgcc ctctgccttt tgccctctgc cctctgttct
     3961 ctggcctctg ccctctgccc tctggcctct ggcctctgcc tctgcctctt gagtgctgga
     4021 atcaaaggtg tgagctctgt aggtcttaag ttccagaaga aagtaatgaa gtcacccagc
     4081 agggaggtgc tcagggacag cacagacaca cacccaggac ataggctccc acttccttgg
     4141 ctttctctga gtggcaaagg accttaggca gtgtcactcc ctaagagaag gggataaaga
     4201 gaggggctga ggtattcatc atgtgctccg tggatctcaa gccctcaagg taaatgggga
     4261 cccacctgtc ctaccagctg gctgacctgt agctttcccc accacagaat ccaagtcgga
     4321 actcttggca cctagaggat ctcgagcgta actataacgg tcctaaggta gcgaacctag
     4381 ggaagttcct attcttcaaa tagtatagga acttccggcg cgcgaagttc ctattctcta
     4441 gaaggtatag gaacttcagc gctaggaagt tcctattctc taaaaagtat aggaacttct
     4501 acatcgccac catggctcct aagaagaaga ggaaggtgat gagcagcggc gccctgctgt
     4561 tccacggcaa gatcccctac gtggtggaga tggagggcaa tgtggatggc cacaccttca
     4621 gcatccgcgg caagggctac ggcgatgcca gcgtgggcaa ggtggatgcc cagttcatct
     4681 gcaccaccgg cgatgtgccc gtgccctgga gcaccctggt gaccaccctg accgcaggcg
     4741 cccagtgctt cgccaagtac ggccccgagc tgaaggattt ctacaagagc tgcatgcccg
     4801 atggctacgt gcaggagcgc accatcacct tcgagggtga tggcaatttc aagacccgcg
     4861 ccgaggtgac cttcgagaat ggcagcgtgt acaatcgcgt gaagctgaat ggccagggct
     4921 tcaagaagga tggccacgtg ctgggcaaga atctggagtt caatttcacc ccccactgcc
     4981 tgtacatctg gggcgatcag gccaatcacg gcctgaagag cgccttcaag atctgccacg
     5041 agatcaccgg cagcaagggc gatttcatcg tggccgatca cacccagatg aataccccca
     5101 tcggcggcgg ccccgtgcac gtgcccgagt accaccacat gagctaccac gtgaagctga
     5161 gcaaggatgt gaccgatcac cgcgataata tgagcctgaa ggagaccgtg cgcgccgtgg
     5221 attgccgcaa gacctacctg tgaagcttaa ttagctgagc ttggactcct gttgatagat
     5281 ccagtaatga cctcagaact ccatctggcc gcgactctag atcataatca gccataccac
     5341 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5401 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5521 tttgtccaaa ctcatcaatg tatcttaagg cgtgctagcc ggaagttcct attcttcaaa
     5581 tagtatagga acttccaacg ttgccaccat gggcagcctg caggacagcg aggtgaacca
     5641 ggaggccaag cccgaggtga aacctgaagt caagccagag acccacatca acctgaaggt
     5701 gagcgacggc agcagcgaga tcttcttcaa gatcaagaag accacccccc tgaggaggct
     5761 gatggaggcc ttcgccaaga ggcagggcaa ggagatggac agcctgacct tcctgtacga
     5821 cggcatcgag atccaggccg accagacccc cgaggacctg gacatggagg acaacgacat
     5881 catcgaggcc cacagggagc agatcggcgg aagcgcaggc gctatggtga gcaagggcga
     5941 ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     6001 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt
     6061 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     6121 gtcccctcat ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     6181 ctacttcaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga
     6241 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6301 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat
     6361 gggctgggag gcctcctccg agcggatgta ccccgaggac ggtgccctga agggcaagat
     6421 caagatgagg ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta
     6481 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat
     6541 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6601 ctccaccggc ggcatggacg agctgtacaa gagtgcacgc gtgagtaagc tgaaccctcc
     6661 tgatgagagt ggccccggct gcatgagctg caagtgtgtg ctctcctgag cggccgcgac
     6721 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     6781 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     6841 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6901 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtgt
     6961 ttaaacgaag ttcctattct ctagaaggta taggaacttc ccgtacggcc accatgggca
     7021 gcctgcagga cagcgaggtg aaccaggagg ccaagcccga ggtgaaacct gaagtcaagc
     7081 cagagaccca catcaacctg aaggtgagcg acggcagcag cgagatcttc ttcaagatca
     7141 agaagaccac ccccctgagg aggctgatgg aggccttcgc caagaggcag ggcaaggaga
     7201 tggacagcct gaccttcctg tacgacggca tcgagatcca ggccgaccag acccccgagg
     7261 acctggacat ggaggacaac gacatcatcg aggcccacag ggagcagatc ggcggaagcg
     7321 caggcgctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     7381 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     7441 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     7501 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     7561 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     7621 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     7681 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     7741 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     7801 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     7861 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     7921 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     7981 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     8041 acaagagtgc acgcgtaagt aagctgaacc ctcctgatga gagtggcccc ggctgcatga
     8101 gctgcaagtg tgtgctctcc tgagcggccg cgactctaga tcataatcag ccataccaca
     8161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8341 ttgtccaaac tcatcaatgt atcttaaggc gtgaagatct tcaggaagtt cctattctct
     8401 aaaaagtata ggaacttcca gtactgccac catgggcagc ctgcaggaca gcgaggtgaa
     8461 ccaggaggcc aagcccgagg tgaaacctga agtcaagcca gagacccaca tcaacctgaa
     8521 ggtgagcgac ggcagcagcg agatcttctt caagatcaag aagaccaccc ccctgaggag
     8581 gctgatggag gccttcgcca agaggcaggg caaggagatg gacagcctga ccttcctgta
     8641 cgacggcatc gagatccagg ccgaccagac ccccgaggac ctggacatgg aggacaacga
     8701 catcatcgag gcccacaggg agcagatcgg cggaagcgca ggcgctatgg tgagcgagct
     8761 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     8821 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     8881 ggcggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     8941 cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     9001 ccccgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     9061 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     9121 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
     9181 cgagaccctg taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct
     9241 cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc
     9301 taagaacctc aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga
     9361 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     9421 ccctagcaaa ctggggcaca gaagtgcacg cgtgagtaag ctgaaccctc ctgatgagag
     9481 tggccccggc tgcatgagct gcaagtgtgt gctctcctga gcggccgcga ctctagatca
     9541 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     9601 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     9661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta ctagctcgag
     9781 gtccttcctc tgcagaggtc ttgcttctcc cggtcagctg actccctccc caagtccttc
     9841 aaatatctca gaacatgggg agaaacgggg accttgtccc tcctaaggaa ccccagtgct
     9901 gcatgccatc atccccccca ccctcgcccc cacccccgcc acttctccct ccatgcatac
     9961 cactagctgt cattttgtac tctgtattta ttccagggct gcttctgatt atttagtttg
    10021 ttctttccct ggagacctgt tagaacataa gggcgtatgg tgggtagggg aggcaggata
    10081 tcagtccctg gggcgagttc ctccctgcca accaagccag atgcctgaaa gagatatgga
    10141 tgagggaagt tggactgtgc ctgtacctgg tacagtcata ctctgttgaa agaatcatcg
    10201 gggagggggg ggggctcaag aggggagagc tctgctgagc ctttgtggac catccaatga
    10261 ggatgagggc ttagattcta ccaggtcatt ctcagccacc acacacaagc gctctgccat
    10321 cactgaagaa gccccctagg gctcttgggc cagggcacac tcagtaaaga tgcaggttca
    10381 gtcagggaat gatggggaaa ggggtaggag gtgggggagg gatcaccccc tcctctaaaa
    10441 cacgagcctg ctgtctccaa aggcctctgc ctgtagtgag ggtggcagaa gaagacaagg
    10501 agccagaact ctgactccag gatctaagtc cgtgcaggaa ggggatccta gaaccatctg
    10561 gttggaccca gcttaccaag ggagagcctt tattcttctt tcccttgccc ctctgtgcca
    10621 gcccctcttg ctgtccctga tcccccagac agcgagagtc ttgcaacctg cctcttccaa
    10681 gacctcctaa tctcaggggc aggcggtgga gtgagatccg gcgtgcacac tttttggaag
    10741 atagctttcc caaggatcct ctcccccact ggcagctctg cctgtcccat caccatgtat
    10801 aataccacca ctgctacagc atctcaccga ggaaagaaaa ctgcacaata aaaccaagcc
    10861 tctggagtgt gtcctggtgt ctgtctcttc tgtgtcctgg cgtctgtctc ttctgtgttc
    10921 ttccaaggtc agaaacaaaa accacacact tcaacctgga tggctcggct gagcacttct
    10981 gtgtgcagaa ggtccaacca gactctgggg taccccggcc ctccctattc ccttgcctcc
    11041 tgtctcccgc tttttatagc tccctatgct gggcttctct ggagagtgaa atctttgccc
    11101 aaatcaatgc gcattctctc tgctgagtca tctggcgaca gcagttgagt tcacccgcca
    11161 acacatgggc ccagctatgt agccgaaccc tggctctgga agtgccaggg actttgtgca
    11221 taagtatgta ccatgccctt ttttcacagt cctagctctg cagaagtgca gcctgaaggc
    11281 ctgtctgctg agaggacatg ccctggagcc ctgaaacagg cacagtggga ggaggaacgg
    11341 aggatgacag gcatcaggcc ctcagtccaa aagcaaccac ttgagaatgg gctggagtac
    11401 gaaacatggg gtcccgtccc tggatccctc ctcaaagagt aataagtaaa atataaacag
    11461 gtaccccagg ccgttctggg tttgggttgt aatgggatcc atttgcagag aactattgag
    11521 acagcccagc cgtactgtga caggcaatgt gggggaggag gttgaatcac ttggtattta
    11581 gcatgaatag aataattccc tgaacatttt tcttaaacat ccatatctaa attaccacca
    11641 ctcgctccca gtcttcctgc ctttgcgcca gcctcctgtc tggccatgcc tgaagaaggc
    11701 tggagaagcc acccacctca ggccatgaca ctgccagcca cttggcaggt gcagccaaac
    11761 ctgagctgtc ccagaaaggg acattctcaa gacccaggca ccctgatcag cactgacttg
    11821 gagctacaag tgtcatgcca gaaaagtctc taagaaaacc ttttcaggga aaagggggtg
    11881 actcaacacc gggcaagttt gggaagcccc acccttcgag tgatggaaga gcagatagga
    11941 agcctcagaa gagagacacc ggcacccagg taacgttcct catgtggtct ctgtcacact
    12001 aggtgctctt ccctggacat ctccgtgacc acactctcag ttcttaggga gatgcgggtg
    12061 ctctctgagg ctatctcaga gttgcagatt ctgaggccta gagtgactac agtcagccta
    12121 ggaagccaca gaggactgtg gaccaggagg gcagaagagg agaagggaag aaaaaccatc
    12181 agataggact tgcaatgaaa ctaacccaag acaatcataa tgcagacagg aatgttaaag
    12241 gcgttcagca gctggccatg acacccatct gtccctctgg ccaagtcagc aagcctggaa
    12301 gacctgggac tcctgcccat atgtcctaag ctccccaccc acccactcgt tcactgtcct
    12361 tattctctct ctaccttcag ccacttagtt tcctacctta agtcctagaa ttgatcctgg
    12421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    12481 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
    12541 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
    12601 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
    12661 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
    12721 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
    12781 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    12841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    12901 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    12961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    13021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    13081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    13141 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    13201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    13261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    13321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    13381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    13441 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    13501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    13561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    13621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    13681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    13741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    13801 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    13861 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    13921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    13981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    14041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    14101 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    14161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    14221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    14281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    14341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    14401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    14461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    14521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgg
//
