LOCUS       sequence-262422-        9999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression vector for rat glucocorticoid receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Schena M, Picard D, Yamamoto KR
  TITLE     Vectors for constitutive and inducible gene expression in yeast.
  JOURNAL   Methods Enzymol. 1991;194:389-98.
  PUBMED    2005799
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "1991"; volume: "194"; pages: "389-98"
COMMENT     Sequence Label: pG/N795
FEATURES             Location/Qualifiers
     source          1..9999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2839..3109
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(3189..3208)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3407..3508
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             3509..4183
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(4279..4296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4279..4295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4288..4310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4504..4523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4623..4645
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4683..4701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4769..4873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4874..4942,4943..5734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5092..5111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5905..6493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6394..6413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6647..6664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6781..6802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6817..6822,6823..6840,6841..6847)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6855..6871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6860..6882
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6879..6895
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6879..6895
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      7247..8589
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    7579..7626
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9280..9923
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     9816..9839
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gtccctggca gtttgcttgg ccaggggagg gggagcgtaa tggactttta taaaagcctg
       61 aggggaggag ctacagtcaa ggtttctgca tcttcgccct cagtggctgc tgcttctcag
      121 gcagattcca agcagcagag gattctcctt gatttctcga aaggctccac aagcaatgtg
      181 cagcagcgac agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
      241 cagcagcagc cagacttatc caaagccgtt tcactgtcca tggggctgta tatgggagag
      301 acagaaacaa aagtgatggg gaatgacttg ggctacccac agcagggcca acttggcctt
      361 tcctctgggg aaacagactt tcggcttctg gaagaaagca ttgcaaacct caataggtcg
      421 accagcgttc cagagaaccc caagagttca acgtctgcaa ctgggtgtgc taccccgaca
      481 gagaaggagt ttcccaaaac tcactcggat gcatcttcag aacagcaaaa tcgaaaaagc
      541 cagaccggca ccaacggagg cagtgtgaaa ttgtatccca cagaccaaag cacctttgac
      601 ctcttgaagg atttggagtt ttccgctggg tccccaggta aagacacaaa cgagagtccc
      661 tggagatcag atctgttgat agatgaaaac ttgctttctc ctttggcggg agaagatgat
      721 ccattccttc tcgaagggga cacgaatgag gattgtaagc ctcttatttt accggacact
      781 aaacctagaa ttaaggatac tggagataca atcttatcaa gtcccagcag tgtggcactg
      841 ccccaagtga aaacagaaaa agatgatttc attgaacttt gcacccccgg ggtaattaag
      901 caagagaaac tgggcccagt ttattgtcag gcaagctttt ctgggacaaa tataattggt
      961 aataaaatgt ctgccatttc tgttcatggt gtgagtacct ctggaggaca gatgtaccac
     1021 tatgacatga atacagcatc cctttctcag cagcaggatc agaagcctgt ttttaatgtc
     1081 attccaccaa ttcctgttgg ttctgaaaac tggaataggt gccaaggctc cggagaggac
     1141 agcctgactt ccttgggggc tctgaacttc ccaggccggt cagtgttttc taatgggtac
     1201 tcaagccctg gaatgagacc agatgtaagc tctcctccat ccagctcgtc agcagccacg
     1261 ggaccacctc ccaagctctg cctggtgtgc tccgatgaag cttcaggatg tcattacggg
     1321 gtgctgacat gtggaagctg caaagtattc tttaaaagag cagtggaagg acagcacaat
     1381 tacctttgtg ctggaagaaa cgattgcatc attgataaaa ttcgaaggaa aaactgccca
     1441 gcatgccgct atcggaaatg tcttcaggct ggaatgaacc ttgaagctcg aaaaacaaag
     1501 aaaaaaatca aagggattca gcaagccact gcaggagtct cacaagacac ttcggaaaat
     1561 cctaacaaaa caatagttcc tgcagcatta ccacagctca cccctacctt ggtgtcactg
     1621 ctggaggtga ttgaacccga ggtgttgtat gcaggatatg atagctctgt tccagattca
     1681 gcatggagaa ttatgaccac actcaacatg ttaggtgggc gtcaagtgat tgcagcagtg
     1741 aaatgggcaa aggcgatacc aggcttcaga aacttacacc tggatgacca aatgaccctg
     1801 ctacagtact catggatgtt tctcatggca tttgccctgg gttggagatc atacagacaa
     1861 tcaagtggaa acctgctctg ctttgctcct gatctgatta ttaatgagca gagaatgtct
     1921 ctaccctgca tgtatgacca atgtaaacac atgctgtttg tctcctctga attacaaaga
     1981 ttgcaggtat cctatgaaga gtatctctgt atgaaaacct tactgcttct ctcctcagtt
     2041 cctaaggaag gtctgaagag ccaagagtta tttgatgaga ttcgaatgac ttatatcaaa
     2101 gagctaggaa aagccatcgt caaaagggaa gggaactcca gtcagaactg gcaacggttt
     2161 taccaactga caaagcttct ggactccatg catgaggtgg ttgagaatct ccttacctac
     2221 tgcttccaga catttttgga taagaccatg agtattgaat tcccagagat gttagctgaa
     2281 atcatcacta atcagatacc aaaatattca aatggaaata tcaaaaagct tctgtttcat
     2341 caaaaatgac tgccttacta agaaaggttg ccttaaagaa agttgaattt atagctttta
     2401 ctgtacaaac ttatcaattt gtcttgtaga tgttttgttg ttctttttgt ttctgtcttg
     2461 ttttgtttta aacacgcact acatgtggtt tatagagggc caagacttgg cgacagaagc
     2521 agttgagtca acactctgaa gtgatagcac agcagacagt gaagtgtatt ggttggtgta
     2581 tcacagaaac taacagttac gtggaggcat ggccactgtc agagagggac cgcacctaaa
     2641 ccaccagtgc ccaaagtcca tgtgatcaac tttctgctca gactttcagt tggctgggta
     2701 aaactttcta gaggatccgt cgacctagat aagtaatgat ccgggatctc ccatgtctct
     2761 actggtggtg gtgcttcttt ggaattattg gaaggtaagg aattgccagg tgttgctttc
     2821 ttatccgaaa agaaataaat tgaattgaat tgaaatcgat agatcaattt ttttcttttc
     2881 tctttcccca tcctttacgc taaaataata gtttatttta ttttttgaat attttttatt
     2941 tatatacgta tatatagact attatttatc ttttaatgat tattaagatt tttattaaaa
     3001 aaaaattcgc tcctctttta atgcctttat gcagtttttt tttcccattc gatatttcta
     3061 tgttcgggtt cagcgtattt taagtttaat aactcgaaaa ttctgcgttc gttaaagctc
     3121 cggatcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     3181 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     3241 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     3301 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     3361 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaaatt cggtcgaaaa
     3421 aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt
     3481 gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg
     3541 tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc
     3601 cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt
     3661 tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa
     3721 atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga
     3781 ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt
     3841 cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag
     3901 tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac
     3961 aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga
     4021 aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     4081 attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     4141 ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     4201 tttatttaag tattgtttgt gcacttgcct gcaggccttt tgaaaagcaa gcataaaaga
     4261 tctgcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4321 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4381 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     4441 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     4501 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4621 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4681 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4741 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4861 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4981 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5041 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5161 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5281 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5401 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5461 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6541 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6601 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6661 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6721 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6781 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6841 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     6901 tacgaattct gaaccagtcc taaaacgagt aaataggacc ggcaattctt caagcaataa
     6961 acaggaatac caattattaa aagataactt agtcagatcg tacaataaag ctttgaagaa
     7021 aaatgcgcct tattcaatct ttgctataaa aaatggccca aaatctcaca ttggaagaca
     7081 tttgatgacc tcatttcttt caatgaaggg cctaacggag ttgactaatg ttgtgggaaa
     7141 ttggagcgat aagcgtgctt ctgccgtggc caggacaacg tatactcatc agataacagc
     7201 aatacctgat cactacttcg cactagtttc tcggtactat gcatatgatc caatatcaaa
     7261 ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc atatagaaca
     7321 gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga taatatcaca
     7381 ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac gcatttaagc
     7441 ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat
     7501 aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct
     7561 cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag aaagtatagg
     7621 aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa
     7681 aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc
     7741 tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca
     7801 cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg tttatctcta
     7861 gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca
     7921 atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt
     7981 cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc
     8041 gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg
     8101 cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga
     8161 aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc
     8221 aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag
     8281 aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt
     8341 ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
     8401 tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct
     8461 tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt
     8521 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga
     8581 tgcttcgtta acaaagatat gctattgaag tgcaagatgg aaacgcagaa aatgaaccgg
     8641 ggatgcgacg tgcaagatta cctatgcaat agatgcaata gtttctccag gaaccgaaat
     8701 acatacattg tcttccgtaa agcgctagac tatatattat tatacaggtt caaatatact
     8761 atctgtttca gggaaaactc ccaggttcgg atgttcaaaa ttcaatgatg ggtaacaagt
     8821 acgatcgtaa atctgtaaaa cagtttgtcg gatattaggc tgtatctcct caaagcgtat
     8881 tcgaatatca ttgagaagct gcagctattc atgcaactgc taaatggtct actgctcaat
     8941 taaagaaaac acaggcagag caggctgccc gggcaaaagc tgcagcgtca catcggataa
     9001 taatgatggc agccattgta gaagtgcctt ttgcatttct agtctctttc tcggtctagc
     9061 tagttttact acatcgcgaa gatagaatct tagatcacac tgcctttgct gagctggatc
     9121 aatagagtaa caaaagagtg gtaaggcctc gttaaaggac aaggacctga gcggaagtgt
     9181 atcgtacagt agacggagta tactagtata gtctatagtc cgtggaattc tcatgtttga
     9241 cagcttatca tcgataagct tggctgcagg tcgagtttat cattatcaat actgccattt
     9301 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
     9361 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
     9421 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
     9481 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
     9541 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
     9601 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
     9661 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
     9721 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
     9781 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
     9841 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
     9901 aaacaccaag aacttagttt cgacggatcc cccccgggct cacattaata tttgccaatg
     9961 gactccaaag aatccttagc tccccctggt agagacgaa
//
