LOCUS       sequence-26244-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag AKT1
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgagcgacg tggctattgt
      181 gaaggagggt tggctgcaca aacgagggga gtacatcaag acctggcggc cacgctactt
      241 cctcctcaag aatgatggca ccttcattgg ctacaaggag cggccgcagg atgtggacca
      301 acgtgaggct cccctcaaca acttctctgt ggcgcagtgc cagctgatga agacggagcg
      361 gccccggccc aacaccttca tcatccgctg cctgcagtgg accactgtca tcgaacgcac
      421 cttccatgtg gagactcctg aggagcggga ggagtggaca accgccatcc agactgtggc
      481 tgacggcctc aagaagcagg aggaggagga gatggacttc cggtcgggct cacccagtga
      541 caactcaggg gctgaagaga tggaggtgtc cctggccaag cccaagcacc gcgtgaccat
      601 gaacgagttt gagtacctga agctgctggg caagggcact ttcggcaagg tgatcctggg
      661 tgaaggagaa ggccacaggc cgctactacg ccatgaagat cctc
//
