LOCUS       sequence-262465-        8515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian 
            cells. Used to modify genome of mouse embroys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Fujii W, Kakuta S, Yoshioka S, Kyuwa S, Sugiura K, Naito K
  TITLE     Zygote-mediated generation of genome-modified mice using 
            Streptococcus thermophilus 1-derived CRISPR/Cas system.
  JOURNAL   Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3.
            doi: 10.1016/j.bbrc.2016.06.070.
  PUBMED    27318086
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3. doi: 
            10.1016/j.bbrc.2016.06.070."
COMMENT     Sequence Label: pCAG-T3-st1CAS9-pA
FEATURES             Location/Qualifiers
     source          1..8515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             117..137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             141..3503
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MSDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLV
                     RRTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEE
                     LFIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYG
                     QLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGK
                     RKYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLL
                     NDLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRID
                     KSGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADG
                     SFSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKT
                     TSSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNED
                     DEKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERC
                     LYTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALD
                     SMDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTLYASRVV
                     LNALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNL
                     WKKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSY
                     QVDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSK
                     FLMYRHDPQTFEKVIEPILENYPNKQINDKGKEVPCNPFLKYKEEHGYIRKYSKKGNGP
                     EIKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQ
                     FDKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRT
                     MPKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQ
                     HIIKNEGDKPKLDF"
     CDS             3516..3536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3822..3841)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3887..3942
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3941..3960)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4303..4319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4303..4319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4316..4338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4327..4343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4351..4357,4358..4375,4376..4381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4396..4417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4476..4671
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4522..4657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4677..4811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4714..4733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4768..4787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4879..4896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5050..5638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5130..5149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5809..6600,6601..6669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6432..6451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6670..6774)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6805..7184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7186..7442
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          7443..8459
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8383..8405
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8467..8486
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgcgaatt
       61 cactagtgat tggatccacc atggattaca aggatgacga cgataagatc atggccccaa
      121 agaagaagcg gaaggtcggt atgagcgacc tggtgctggg cctggacatc ggcatcggca
      181 gcgtgggcgt gggcatcctg aacaaggtga ccggcgagat catccacaag aacagtcgca
      241 tcttccctgc tgctcaggct gagaacaacc tggtgcgccg caccaaccgc cagggtcgcc
      301 ggcttgctcg ccgcaagaag caccggcgcg tgcgcctgaa ccgcctgttc gaggagagcg
      361 gcctgatcac cgacttcacc aagatcagca tcaacctgaa cccctaccag ctgcgcgtga
      421 agggcctgac cgacgagctg agcaacgagg agctgttcat cgccctgaag aacatggtga
      481 agcaccgcgg catcagctac ctggacgacg ccagcgacga cggcaacagc agcgtgggcg
      541 actacgccca gatcgtgaag gagaacagca agcagctgga gaccaagacc cccggccaga
      601 tccagctgga gcgctaccag acctacggcc agctgcgcgg cgacttcacc gtggagaagg
      661 acggcaagaa gcaccgcctg atcaacgtgt tccccaccag cgcctaccgc agcgaggccc
      721 tgcgcatcct gcagacccag caggagttca acccccagat caccgacgag ttcatcaacc
      781 gctacctgga gatcctgacc ggcaagcgca agtactacca cggccccggc aacgagaaga
      841 gccgcaccga ctacggccgc taccgcacca gcggcgagac cctggacaac atcttcggca
      901 tcctgatcgg caagtgcacc ttctaccccg acgagttccg cgccgccaag gccagctaca
      961 ccgcccagga gttcaacctg ctgaacgacc tgaacaacct gaccgtgccc accgagacca
     1021 agaagctgag caaggagcag aagaaccaga tcatcaacta cgtgaagaac gagaaggcca
     1081 tgggccccgc caagctgttc aagtacatcg ccaagctgct gagctgcgac gtggccgaca
     1141 tcaagggcta ccgcatcgac aagagcggca aggccgagat ccacaccttc gaggcctacc
     1201 gcaagatgaa gaccctggag accctggaca tcgagcagat ggaccgagag accctggaca
     1261 agctggccta cgtgctgacc ctgaacaccg agcgcgaggg catccaggag gccctggagc
     1321 acgagttcgc cgacggcagc ttcagccaga agcaggtgga cgagctggtg cagttccgca
     1381 aggccaacag cagcatcttc ggcaagggct ggcacaactt cagcgtgaag ctgatgatgg
     1441 agctgatccc cgagctgtac gagaccagcg aggagcagat gaccatcctg acccgcctgg
     1501 gcaagcagaa gaccaccagc agcagcaaca agaccaagta catcgacgag aagctgctga
     1561 ccgaggagat ctacaacccc gtggtggcca agagcgtgcg ccaggccatc aagatcgtga
     1621 acgccgccat caaggagtac ggcgacttcg acaacatcgt gatcgagatg gcccgcgaga
     1681 ccaacgagga cgacgagaag aaggccatcc agaagatcca gaaggccaac aaggacgaga
     1741 aggacgccgc catgctgaag gccgccaacc agtacaacgg caaggccgag ctgccccaca
     1801 gcgtgttcca cggccacaag cagctggcca ccaagatccg cctgtggcac cagcagggcg
     1861 agcgctgcct gtacaccggc aagaccatca gcatccacga cctgatcaac aacagcaacc
     1921 agttcgaggt ggaccacatc ctgcccctga gcatcacctt cgacgacagc ctggccaaca
     1981 aggtgctggt gtacgccacc gccaaccagg agaagggcca gcgcaccccc taccaggccc
     2041 tggacagcat ggacgacgcc tggagcttcc gcgagctgaa ggccttcgtg cgcgagagca
     2101 agaccctgag caacaagaag aaggagtacc tgctgaccga ggaggacatc agcaagttcg
     2161 acgtgcgcaa gaagttcatc gagcgcaacc tggtggacac cctctacgcc agccgcgtgg
     2221 tgctgaacgc cctgcaggag cacttccgcg cccacaagat cgacaccaag gtgagcgtgg
     2281 tgcgcggcca gttcaccagc cagctgcgcc gccactgggg catcgagaag acccgcgaca
     2341 cctaccacca ccacgccgtg gacgccctga tcattgcggc ttctagccag ctgaacctgt
     2401 ggaagaagca gaagaacacc ctggtgagct acagcgagga ccagctgctg gacatcgaga
     2461 ccggcgagct gatcagcgac gacgagtaca aggagagcgt gttcaaggcc ccctaccagc
     2521 acttcgtgga caccctgaag agcaaggagt tcgaggacag catcctgttc agctaccagg
     2581 tggacagcaa gttcaaccgc aagatcagcg acgccaccat ctacgccacc cgccaggcca
     2641 aggtgggcaa ggacaaggcc gacgagacct acgtgctggg caagatcaag gacatctaca
     2701 cccaggacgg ctacgacgcc ttcatgaaga tctacaagaa ggacaagagc aagttcctga
     2761 tgtaccgcca cgacccccag accttcgaga aggtgatcga gcccatcctg gagaactacc
     2821 ccaacaagca gatcaacgat aaaggcaagg aggtgccctg caaccccttc ctgaagtaca
     2881 aggaggagca cggctacatc cgcaagtaca gcaagaaggg caacggcccc gagatcaaga
     2941 gcctgaagta ctacgacagc aagctgggca accacatcga catcaccccc aaggacagca
     3001 acaacaaggt ggtgctgcag agcgtgagcc cctggcgcgc cgacgtgtac ttcaacaaga
     3061 ccaccggcaa gtacgagatc ctgggcctga agtacgccga cctgcagttt gataagggca
     3121 ccggcaccta caagatcagc caggagaagt acaacgacat caagaagaag gagggcgtgg
     3181 acagcgacag cgagttcaag ttcaccctgt acaagaacga ccttctgctg gtgaaggaca
     3241 ccgagaccaa ggagcaacag ctgttccgct tcctgagccg caccatgccc aagcagaagc
     3301 actacgtgga gctgaagccc tacgacaagc agaagttcga gggcggcgag gccctgatca
     3361 aggtgctggg caacgtggcc aacagcggcc agtgcaagaa gggcctgggc aagagcaaca
     3421 tcagcatcta caaggtgcgc accgacgtgc tgggcaacca gcacatcatc aagaacgagg
     3481 gcgacaagcc caagttggac ttcagcaggg ctgaccccaa gaagaagagg aaggtgtgaa
     3541 tcgatggtac ctatgggcac caaagaacct gtaaacgtta tctttttaaa ttgaatgtgc
     3601 acaaataaaa gwwwrraaaa raaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3661 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaactcgag
     3721 gtagcatgca agtgtacgga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     3781 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     3841 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     3901 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     3961 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4021 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     4081 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     4141 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     4201 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     4261 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     4321 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4381 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4441 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     4501 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4621 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     4681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4801 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4861 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4921 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4981 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5461 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5701 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5761 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5821 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5881 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5941 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6001 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6061 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6121 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6181 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6241 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6301 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6361 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6421 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6481 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6541 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6601 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6661 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6721 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6781 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
     6841 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     6901 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     6961 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7021 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7081 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7141 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     7201 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     7261 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggsgs ggcgmggggc
     7321 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     7381 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     7441 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     7501 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     7561 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     7621 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     7681 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     7741 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     7801 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     7861 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     7921 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     7981 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     8041 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     8101 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     8161 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     8221 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     8281 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     8341 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     8401 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     8461 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcga
//
