LOCUS       sequence-262507-        1992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for stable transfection of 
            eukaryotic cells with luciferse and hygromycin restistance. CMV 
            protomor drives SB100X transposase for transposon integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF112
FEATURES             Location/Qualifiers
     source          1..1992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        296..499
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     450..470
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     496..520
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          637..733
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     790..809
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        790..808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             819..1841
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    1858..1992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1882..1901)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1936..1955
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
       61 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      121 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      181 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      241 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      301 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      361 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      421 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      481 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      541 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagaggatcc
      601 ggtactcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt tttgtctttt
      661 atttcaggtc ccggatccgg tggtggtgca aatcaaagaa ctgctcctca gtggatgttg
      721 cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc ggaattgtac
      781 ccgcggaatt aatacgactc actataggga ctagtaccat gggaaaatca aaagaaatca
      841 gccaagacct cagaaaaaga attgtagacc tccacaagtc tggttcatcc ttgggagcaa
      901 tttccaaacg cctggcggta ccacgttcat ctgtacaaac aatagtacgc aagtataaac
      961 accatgggac cacgcagccg tcataccgct caggaaggag acgcgttctg tctcctagag
     1021 atgaacgtac tttggtgcga aaagtgcaaa tcaatcccag aacaacagca aaggaccttg
     1081 tgaagatgct ggaggaaaca ggtacaaaag tatctatatc cacagtaaaa cgagtcctat
     1141 atcgacataa cctgaaaggc cactcagcaa ggaagaagcc actgctccaa aaccgacata
     1201 agaaagccag actacggttt gcaactgcac atggggacaa agatcgtact ttttggagaa
     1261 atgtcctctg gtctgatgaa acaaaaatag aactgtttgg ccataatgac catcgttatg
     1321 tttggaggaa gaagggggag gcttgcaagc cgaagaacac catcccaacc gtgaagcacg
     1381 ggggtggcag catcatgttg tgggggtgct ttgctgcagg agggactggt gcacttcaca
     1441 aaatagatgg catcatggac gcggtgcagt atgtggatat attgaagcaa catctcaaga
     1501 catcagtcag gaagttaaag cttggtcgca aatgggtctt ccaacacgac aatgacccca
     1561 agcatacttc caaagttgtg gcaaaatggc ttaaggacaa caaagtcaag gtattggagt
     1621 ggccatcaca aagccctgac ctcaatccta tagaaaattt gtgggcagaa ctgaaaaagc
     1681 gtgtgcgagc aaggaggcct acaaacctga ctcagttaca ccagctctgt caggaggaat
     1741 gggccaaaat tcacccaaat tattgtggga agcttgtgga aggctacccg aaacgtttga
     1801 cccaagttaa acaatttaaa ggcaatgcta ccaaatacta ggggccctaa ccgcggggat
     1861 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1921 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1981 aataaacaag tt
//
