LOCUS       sequence-262509-        2001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for stable transfection of 
            eukaryotic cells with hygromycin restistance. CMV protomor drives 
            SB100X transposase for transposon integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF123
FEATURES             Location/Qualifiers
     source          1..2001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     799..818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        799..817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             828..1850
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    1867..2001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1891..1910)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1945..1964
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactcgagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcggaatta atacgactca ctatagggac tagtaccatg ggaaaatcaa
      841 aagaaatcag ccaagacctc agaaaaagaa ttgtagacct ccacaagtct ggttcatcct
      901 tgggagcaat ttccaaacgc ctggcggtac cacgttcatc tgtacaaaca atagtacgca
      961 agtataaaca ccatgggacc acgcagccgt cataccgctc aggaaggaga cgcgttctgt
     1021 ctcctagaga tgaacgtact ttggtgcgaa aagtgcaaat caatcccaga acaacagcaa
     1081 aggaccttgt gaagatgctg gaggaaacag gtacaaaagt atctatatcc acagtaaaac
     1141 gagtcctata tcgacataac ctgaaaggcc actcagcaag gaagaagcca ctgctccaaa
     1201 accgacataa gaaagccaga ctacggtttg caactgcaca tggggacaaa gatcgtactt
     1261 tttggagaaa tgtcctctgg tctgatgaaa caaaaataga actgtttggc cataatgacc
     1321 atcgttatgt ttggaggaag aagggggagg cttgcaagcc gaagaacacc atcccaaccg
     1381 tgaagcacgg gggtggcagc atcatgttgt gggggtgctt tgctgcagga gggactggtg
     1441 cacttcacaa aatagatggc atcatggacg cggtgcagta tgtggatata ttgaagcaac
     1501 atctcaagac atcagtcagg aagttaaagc ttggtcgcaa atgggtcttc caacacgaca
     1561 atgaccccaa gcatacttcc aaagttgtgg caaaatggct taaggacaac aaagtcaagg
     1621 tattggagtg gccatcacaa agccctgacc tcaatcctat agaaaatttg tgggcagaac
     1681 tgaaaaagcg tgtgcgagca aggaggccta caaacctgac tcagttacac cagctctgtc
     1741 aggaggaatg ggccaaaatt cacccaaatt attgtgggaa gcttgtggaa ggctacccga
     1801 aacgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactag gggccctaac
     1861 cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1921 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1981 ataagctgca ataaacaagt t
//
