LOCUS       sequence-262515-        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF139
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             580..600
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             634..651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             658..1359
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atggtaaaca gctacaatag actggaggcg atatcaactc ttgttatact actctgggta
       61 actctggtcc agggttcctg tggcagctgt ccagctggta ctttctggaa tggtacagat
      121 tgtggaaaaa acaaaaatca agcttgcatc ccatgtcccc caaatagttt ctctagtaca
      181 gctggcgaac agaggacatg taacatatgc aggagatgtg aaggtatttt tagaacaaag
      241 agagaatgta ctccaacaag taatacagag tgtgattgta tgtctgggta tcattgttcc
      301 ggagtaggat gtaataggtg tcaaccagat tgcaagcaag gtcaagaact tctaggaaaa
      361 ggttgtagag actgtagctt tggtaccttt aatgatcaga cacgggggaa ctgcaaacgc
      421 tggacaaact gctctttgaa tggactagtt gtgcttgtaa atggaaccaa taaaagagat
      481 gtgatatgtg gatccaccca aacagttgcc actctggaca caatctccat gacaattcca
      541 gcttctctcg tgcacattga taagaaccat cagcccgggg agaaccttta cttccaggga
      601 tccgctggct ccgctgctgg ttctggcgaa ttccatcatc accatcacca tgtcgacatg
      661 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
      721 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
      781 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
      841 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
      901 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
      961 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     1021 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
     1081 gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
     1141 ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag
     1201 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
     1261 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
     1321 tgcgacctcc ctagcaaact ggggcacaag ctgaactag
//
