LOCUS       sequence-262521-        1389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF163
FEATURES             Location/Qualifiers
     source          1..1389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             580..600
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             634..651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             658..1389
                     /codon_start=1
                     /product="far-red fluorescent protein with spectra similar 
                     to those of mNeptune but improved brightness"
                     /label=mNeptune2
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGR
                     IKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCDM
                     ALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 atggtaaaca gctacaatag actggaggcg atatcaactc ttgttatact actctgggta
       61 actctggtcc agggttcctg tggcagctgt ccagctggta ctttctggaa tggtacagat
      121 tgtggaaaaa acaaaaatca agcttgcatc ccatgtcccc caaatagttt ctctagtaca
      181 gctggcgaac agaggacatg taacatatgc aggagatgtg aaggtatttt tagaacaaag
      241 agagaatgta ctccaacaag taatacagag tgtgattgta tgtctgggta tcattgttcc
      301 ggagtaggat gtaataggtg tcaaccagat tgcaagcaag gtcaagaact tctaggaaaa
      361 ggttgtagag actgtagctt tggtaccttt aatgatcaga cacgggggaa ctgcaaacgc
      421 tggacaaact gctctttgaa tggactagtt gtgcttgtaa atggaaccaa taaaagagat
      481 gtgatatgtg gatccaccca aacagttgcc actctggaca caatctccat gacaattcca
      541 gcttctctcg tgcacattga taagaaccat cagcccgggg agaaccttta cttccaggga
      601 tccgctggct ccgctgctgg ttctggcgaa ttccatcatc accatcacca tgtcgacgtg
      661 tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
      721 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      781 cagaccggca gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      841 gctacctgct tcatgtacgg cagcaagacc ttcatcaacc acacccaggg catccccgat
      901 ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
      961 gggggcgtgc ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac
     1021 gtcaagctca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
     1081 ggctgggagg ccagtaccga gaccctgtac cccgctgacg gcggcctgga aggcagatgc
     1141 gacatggccc tgaagctcgt gggcgggggc cacctgcact gcaacctgaa gaccacatac
     1201 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactttgt ggaccgcaga
     1261 ctggaaagaa tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg
     1321 gccagatact gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg
     1381 tacaagtag
//
