LOCUS       sequence-262523-        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF164
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             580..600
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             634..651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             658..1365
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1068..1087
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtaaaca gctacaatag actggaggcg atatcaactc ttgttatact actctgggta
       61 actctggtcc agggttcctg tggcagctgt ccagctggta ctttctggaa tggtacagat
      121 tgtggaaaaa acaaaaatca agcttgcatc ccatgtcccc caaatagttt ctctagtaca
      181 gctggcgaac agaggacatg taacatatgc aggagatgtg aaggtatttt tagaacaaag
      241 agagaatgta ctccaacaag taatacagag tgtgattgta tgtctgggta tcattgttcc
      301 ggagtaggat gtaataggtg tcaaccagat tgcaagcaag gtcaagaact tctaggaaaa
      361 ggttgtagag actgtagctt tggtaccttt aatgatcaga cacgggggaa ctgcaaacgc
      421 tggacaaact gctctttgaa tggactagtt gtgcttgtaa atggaaccaa taaaagagat
      481 gtgatatgtg gatccaccca aacagttgcc actctggaca caatctccat gacaattcca
      541 gcttctctcg tgcacattga taagaaccat cagcccgggg agaaccttta cttccaggga
      601 tccgctggct ccgctgctgg ttctggcgaa ttccatcatc accatcacca tgtcgacgtg
      661 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcgc
      721 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      781 tacgagggct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      841 tgggacatcc tgtcccctca gttcacctac ggctccaagg cctacgtgaa gcaccccgcc
      901 gacatccccg actacttcaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      961 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     1021 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1081 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1141 aagggcgaga tcaagatgag gctgaagctg aaggacggcg gccactacac ctccgaggtc
     1201 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcggcatc
     1261 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     1321 gagggccgcc actccaccgg cggcatggac gagctgtaca agtag
//
