LOCUS       sequence-262534-       10145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. CTLA4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF197
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1134..1153
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1152..1207
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1253..1272
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        3029..4211
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     4159..4179
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4240..4249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5425..5445
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5479..5496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5503..6213
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5648..5666)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5916..5935
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6265..6282)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6271..6495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6857..7160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7161..7364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7315..7335
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7361..7385
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          7502..7598
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     7655..7674
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7655..7673
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7684..8706
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8723..8857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8747..8766)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8801..8820
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9010..9114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9115..9183,9184..9975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9333..9352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatggt tctcctgaga
     4261 tccaggagac aaatgggaaa gcttcaccat cccaagaact ggccctgcac agcaatgctc
     4321 tctcttctct ttattccaag tatctctaaa ggggtacatg tgactcaacc agaagtggtc
     4381 ttggccaaca gcagaggggt tgccagcttt gtgtgtaaat atgaattgac aaacaaaacc
     4441 aaagaggtcc gagtggggct tcttcgacag atggacaacc agatggttga agtctgtgcc
     4501 tcaacatatc tggtacagga tcaaccagtg ttcatggatg acatgcttga gtgtacggga
     4561 catgtcaatg ggagtaaagt gacgctcact ctcaccgggc taaaggcctc agacagtgga
     4621 ctatacatct gcaaagtgga actcatgtat cctccaccct actacatggg tttgggcaat
     4681 ggaacacaaa tatatgtaat tgatgctgaa ccttgtccag actttgaaga tatcccaaag
     4741 cccacagaat gtacatgctg caaatgctca gcatgtgatg ttggtggact ttctgtattc
     4801 atcttccccc caaatcctaa ggataccctc aaactctcag gatcacctaa aatcacctgt
     4861 atggtggttg atgtgagtga tgcatcagat gttaaaattg cctggttcaa aaataataag
     4921 gaaattgaca gtcctaaacc aacacagaag gaacaaaaca atgggaccat tcaggtgatc
     4981 agcactctct ctgtagtcca ccaagaatgg ctgaaaggca cagtgtatac ctgtaaagtt
     5041 gataccaaag aattaccatt tcctgaaaca aggaccatct cccacacttc aggtgacaga
     5101 aagatgcctg atatgtatgt ctttgcccca catcctgatg agttgaagca taaggatact
     5161 gttagtctta cctgcttcgt aaaaaatttc ttccctcaag acattgttgt tgaatggcaa
     5221 tgcaatgacc atccagagcc tgaagaaaac tattatacca ccccagcaat gaaggaaaag
     5281 gacaccttct ttgtctacag caaactcact ctgaataaag caaaatggca agagaataac
     5341 tcctatacct gcatggtact gcatgagggc tttccaaacc aatattccca gaggacaatc
     5401 tctgctagcc caggtaaacc cggggagaac ctttacttcc agggatccgc tggctccgct
     5461 gctggttctg gcgaattcca tcatcaccat caccatgtcg acatggtgag caagggcgag
     5521 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     5581 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5641 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     5701 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     5761 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5821 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     5881 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     5941 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     6001 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     6061 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     6121 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     6181 tccaccggcg gcatggacga gctgtacaag taggctagcg tcaggccaag cttggggatc
     6241 aattctctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6301 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6361 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6421 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6481 cggtgggctc tatggcttct gaggcggaaa gaaccagctg ggcctactag atagcggacc
     6541 ccttaccgaa acatcgccgc attctgcaga ggagtcgaac ttagtgtatg taaacttctg
     6601 acccactgga attgtgatac agtgaattat aagtgaaata atctgtctgt aaacaattgt
     6661 tggaaaaatg acttgtgtca tgcacaaagt agatgtccta actgacttgc caaaactatt
     6721 gtttgttaac aagaaatttg tggagtagtt gaaaaacgag ttttaatgac tccaacttaa
     6781 gtgtatgtaa acttccgact tcaactgtat agggatccaa ttttaacaaa atattcgagc
     6841 ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     6901 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     6961 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7021 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7081 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7141 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7201 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7261 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7321 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     7381 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     7441 ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag aaagttaact
     7501 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg gtgcaaatca
     7561 aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc
     7621 tgctctaaaa gctgcggaat tgtacccgcg gaattaatac gactcactat agggactagt
     7681 accatgggaa aatcaaaaga aatcagccaa gacctcagaa aaagaattgt agacctccac
     7741 aagtctggtt catccttggg agcaatttcc aaacgcctgg cggtaccacg ttcatctgta
     7801 caaacaatag tacgcaagta taaacaccat gggaccacgc agccgtcata ccgctcagga
     7861 aggagacgcg ttctgtctcc tagagatgaa cgtactttgg tgcgaaaagt gcaaatcaat
     7921 cccagaacaa cagcaaagga ccttgtgaag atgctggagg aaacaggtac aaaagtatct
     7981 atatccacag taaaacgagt cctatatcga cataacctga aaggccactc agcaaggaag
     8041 aagccactgc tccaaaaccg acataagaaa gccagactac ggtttgcaac tgcacatggg
     8101 gacaaagatc gtactttttg gagaaatgtc ctctggtctg atgaaacaaa aatagaactg
     8161 tttggccata atgaccatcg ttatgtttgg aggaagaagg gggaggcttg caagccgaag
     8221 aacaccatcc caaccgtgaa gcacgggggt ggcagcatca tgttgtgggg gtgctttgct
     8281 gcaggaggga ctggtgcact tcacaaaata gatggcatca tggacgcggt gcagtatgtg
     8341 gatatattga agcaacatct caagacatca gtcaggaagt taaagcttgg tcgcaaatgg
     8401 gtcttccaac acgacaatga ccccaagcat acttccaaag ttgtggcaaa atggcttaag
     8461 gacaacaaag tcaaggtatt ggagtggcca tcacaaagcc ctgacctcaa tcctatagaa
     8521 aatttgtggg cagaactgaa aaagcgtgtg cgagcaagga ggcctacaaa cctgactcag
     8581 ttacaccagc tctgtcagga ggaatgggcc aaaattcacc caaattattg tgggaagctt
     8641 gtggaaggct acccgaaacg tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa
     8701 tactaggggc cctaaccgcg gggatccaga catgataaga tacattgatg agtttggaca
     8761 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     8821 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     8881 tatgtttcag gttcaggggg aggtgtggga ggttttttaa cgcttacaat ttcctgatgc
     8941 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg
     9001 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9061 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9121 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9181 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9241 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9301 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9361 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9421 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9481 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9541 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9601 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9661 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9721 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9781 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9841 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9901 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9961 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10021 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10141 tcttc
//
