LOCUS       sequence-262537-        1968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. CTLA4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF197
FEATURES             Location/Qualifiers
     source          1..1968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1180..1200
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1234..1251
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1258..1968
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1403..1421)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1671..1690
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggttctcc tgagatccag gagacaaatg ggaaagcttc accatcccaa gaactggccc
       61 tgcacagcaa tgctctctct tctctttatt ccaagtatct ctaaaggggt acatgtgact
      121 caaccagaag tggtcttggc caacagcaga ggggttgcca gctttgtgtg taaatatgaa
      181 ttgacaaaca aaaccaaaga ggtccgagtg gggcttcttc gacagatgga caaccagatg
      241 gttgaagtct gtgcctcaac atatctggta caggatcaac cagtgttcat ggatgacatg
      301 cttgagtgta cgggacatgt caatgggagt aaagtgacgc tcactctcac cgggctaaag
      361 gcctcagaca gtggactata catctgcaaa gtggaactca tgtatcctcc accctactac
      421 atgggtttgg gcaatggaac acaaatatat gtaattgatg ctgaaccttg tccagacttt
      481 gaagatatcc caaagcccac agaatgtaca tgctgcaaat gctcagcatg tgatgttggt
      541 ggactttctg tattcatctt ccccccaaat cctaaggata ccctcaaact ctcaggatca
      601 cctaaaatca cctgtatggt ggttgatgtg agtgatgcat cagatgttaa aattgcctgg
      661 ttcaaaaata ataaggaaat tgacagtcct aaaccaacac agaaggaaca aaacaatggg
      721 accattcagg tgatcagcac tctctctgta gtccaccaag aatggctgaa aggcacagtg
      781 tatacctgta aagttgatac caaagaatta ccatttcctg aaacaaggac catctcccac
      841 acttcaggtg acagaaagat gcctgatatg tatgtctttg ccccacatcc tgatgagttg
      901 aagcataagg atactgttag tcttacctgc ttcgtaaaaa atttcttccc tcaagacatt
      961 gttgttgaat ggcaatgcaa tgaccatcca gagcctgaag aaaactatta taccacccca
     1021 gcaatgaagg aaaaggacac cttctttgtc tacagcaaac tcactctgaa taaagcaaaa
     1081 tggcaagaga ataactccta tacctgcatg gtactgcatg agggctttcc aaaccaatat
     1141 tcccagagga caatctctgc tagcccaggt aaacccgggg agaaccttta cttccaggga
     1201 tccgctggct ccgctgctgg ttctggcgaa ttccatcatc accatcacca tgtcgacatg
     1261 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     1321 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1381 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1441 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     1501 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1561 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     1621 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1681 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     1741 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     1801 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     1861 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1921 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtag
//
