LOCUS       sequence-26254-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag BTK
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..88
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    143..167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgccctnctc cttctctggc gccggccgga tccagaattc gccaccatgg ggtcttcaaa
       61 atctaaacca aaggacccca gccagcgccg gcgcaggatc cgaggttacc ttgactacaa
      121 agacgatgac gacaagcaat tgacaagttt gtacaaaaaa gttggcatgg ccgcagtgat
      181 tctggagagc atctttctga agcgatccca acagaaaaag aaaacatcac ctctaaactt
      241 caagaagcgc ctgtttctct tgaccgtgca caaactctcc tactatgagt atgactttga
      301 acgtgggaga agaggcagta agaagggttc aatagatgtt gagaagatca cttgtgttga
      361 aacagtggtt cctgaaaaaa atcctcctcc agaaagacag attccgagaa gaggtgaaga
      421 gtccagtgaa atggagcaaa tttcaatcat tgaaaggttc ccttatccct tccaggttgt
      481 atatgatgaa gggcctctct acgtcttctc cccaactgaa gaactaagga agcggtggat
      541 tcaccagctc aaaaacgtaa tccggtacaa cagtgatctg gttcagaaat atcacccttg
      601 cttctggatc gatgggcagt atctctgctg ctctcagaca gccaaaaatg ctatgggctg
      661 ccaaattttg gagaacagga atggaagctt aaaacctggg agttctcacc ggaagacaaa
      721 aaagcctctt cccccacgcc tgaggaggac cagatcttga aaaagccact accgcctgag
      781 ccagcagcag caccagtctc acagtgagct gaaaaggtgt ggcctttatg a
//
