LOCUS       sequence-262543-        9551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF161
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1134..1153
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1152..1207
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1253..1272
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        3029..4211
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     4159..4179
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4240..4249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4825..4845
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4879..4896
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(4903..4905,4906..5619)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKA
                     NFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4945..4966)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5206..5225)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5553..5574
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5671..5688)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5677..5901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6263..6566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6567..6770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6721..6741
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6767..6791
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          6908..7004
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     7061..7080
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7061..7079
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7090..8112
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8129..8263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8153..8172)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8207..8226
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8416..8520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8521..8589,8590..9381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8739..8758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatggt aaacagctac
     4261 aatagactgg aggcgatatc aactcttgtt atactactct gggtaactct ggtccagggt
     4321 tcctgtggca gctgtccagc tggtactttc tggaatggta cagattgtgg aaaaaacaaa
     4381 aatcaagctt gcatcccatg tcccccaaat agtttctcta gtacagctgg cgaacagagg
     4441 acatgtaaca tatgcaggag atgtgaaggt atttttagaa caaagagaga atgtactcca
     4501 acaagtaata cagagtgtga ttgtatgtct gggtatcatt gttccggagt aggatgtaat
     4561 aggtgtcaac cagattgcaa gcaaggtcaa gaacttctag gaaaaggttg tagagactgt
     4621 agctttggta cctttaatga tcagacacgg gggaactgca aacgctggac aaactgctct
     4681 ttgaatggac tagttgtgct tgtaaatgga accaataaaa gagatgtgat atgtggatcc
     4741 acccaaacag ttgccactct ggacacaatc tccatgacaa ttccagcttc tctcgtgcac
     4801 attgataaga accatcagcc cggggagaac ctttacttcc agggatccgc tggctccgct
     4861 gctggttctg gcgaattcca tcatcaccat caccatgtcg acgtgagcaa gggcgaggag
     4921 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4981 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     5041 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagctgg
     5101 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5161 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5221 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5281 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
     5341 cacggcaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcggc
     5401 ctcaactgca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     5461 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     5521 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     5581 gccgggatca ctctcggcat ggacgagctg tacaagtagg ctagcgtcag gccaagcttg
     5641 gggatcaatt ctctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     5701 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5761 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5821 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5881 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctgggcc tactagatag
     5941 cggacccctt accgaaacat cgccgcattc tgcagaggag tcgaacttag tgtatgtaaa
     6001 cttctgaccc actggaattg tgatacagtg aattataagt gaaataatct gtctgtaaac
     6061 aattgttgga aaaatgactt gtgtcatgca caaagtagat gtcctaactg acttgccaaa
     6121 actattgttt gttaacaaga aatttgtgga gtagttgaaa aacgagtttt aatgactcca
     6181 acttaagtgt atgtaaactt ccgacttcaa ctgtataggg atccaatttt aacaaaatat
     6241 tcgagcttgc atgcctgcag gtcgttacat aacttacggt aaatggcccg cctggctgac
     6301 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     6361 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     6421 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     6481 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     6541 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     6601 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     6661 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     6721 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     6781 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     6841 accgatccag cctccggact ctagaggatc cggtactcga ggaactgaaa aaccagaaag
     6901 ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggatccg gtggtggtgc
     6961 aaatcaaaga actgctcctc agtggatgtt gcctttactt ctaggcctgt acggaagtgt
     7021 tacttctgct ctaaaagctg cggaattgta cccgcggaat taatacgact cactataggg
     7081 actagtacca tgggaaaatc aaaagaaatc agccaagacc tcagaaaaag aattgtagac
     7141 ctccacaagt ctggttcatc cttgggagca atttccaaac gcctggcggt accacgttca
     7201 tctgtacaaa caatagtacg caagtataaa caccatggga ccacgcagcc gtcataccgc
     7261 tcaggaagga gacgcgttct gtctcctaga gatgaacgta ctttggtgcg aaaagtgcaa
     7321 atcaatccca gaacaacagc aaaggacctt gtgaagatgc tggaggaaac aggtacaaaa
     7381 gtatctatat ccacagtaaa acgagtccta tatcgacata acctgaaagg ccactcagca
     7441 aggaagaagc cactgctcca aaaccgacat aagaaagcca gactacggtt tgcaactgca
     7501 catggggaca aagatcgtac tttttggaga aatgtcctct ggtctgatga aacaaaaata
     7561 gaactgtttg gccataatga ccatcgttat gtttggagga agaaggggga ggcttgcaag
     7621 ccgaagaaca ccatcccaac cgtgaagcac gggggtggca gcatcatgtt gtgggggtgc
     7681 tttgctgcag gagggactgg tgcacttcac aaaatagatg gcatcatgga cgcggtgcag
     7741 tatgtggata tattgaagca acatctcaag acatcagtca ggaagttaaa gcttggtcgc
     7801 aaatgggtct tccaacacga caatgacccc aagcatactt ccaaagttgt ggcaaaatgg
     7861 cttaaggaca acaaagtcaa ggtattggag tggccatcac aaagccctga cctcaatcct
     7921 atagaaaatt tgtgggcaga actgaaaaag cgtgtgcgag caaggaggcc tacaaacctg
     7981 actcagttac accagctctg tcaggaggaa tgggccaaaa ttcacccaaa ttattgtggg
     8041 aagcttgtgg aaggctaccc gaaacgtttg acccaagtta aacaatttaa aggcaatgct
     8101 accaaatact aggggcccta accgcgggga tccagacatg ataagataca ttgatgagtt
     8161 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     8221 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     8281 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaacgct tacaatttcc
     8341 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
     8401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8641 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8761 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9061 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9541 aaaggatctt c
//
