LOCUS       sequence-262546-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IKZF1-V1-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF1-V1-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          667..1683
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1607..1629
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1691..1710
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1799..1823
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3381..3405)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3425..3448
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3483..4032
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3627..3644)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3854..3873
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4035..4037,4038..4040,4041..4754)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4035..4037,4038..4040,4041..4751)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4080..4101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4341..4360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4688..4709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4831..5419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4884..4904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5302..5313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5422..5438)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5423..5439)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5628..5861
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5933..6054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5970..5989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6024..6043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6094..6229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6249..6268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6250..6268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6291..6649)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6331..6352)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6543..6562
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6676..6780
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6782..7139
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6782..6973
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6805..6825)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6990..7125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7174..7968
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7174..7968
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7228..7247)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7838..7857
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8156..8175)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8200..8247
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8576..9164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9065..9084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9339..9642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9644..9842
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9797..9817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9860..10040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10087..10212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10709..10942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11127..11171
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11469..11586
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gttagtaccg ggcccgctct agccctagat ttaaatcgat gtacgggcca gatatacgcg
       61 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      421 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      481 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      541 gatgggggcg gggsgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
      601 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      661 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      721 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      781 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      841 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
      901 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
      961 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     1021 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     1081 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     1141 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     1201 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     1261 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     1321 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     1381 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     1441 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     1501 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     1561 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     1621 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     1681 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcccc
     1741 tagccgcgga atctagactg ccggatcgaa ttgtcgacga attcggatct ggccaatcac
     1801 aagtttgtac aaaaaagttg gcatggatgc tgatgagggt caagacatgt cccaagtttc
     1861 agggaaggaa agcccccctg taagcgatac tccagatgag ggcgatgagc ccatgccgat
     1921 ccccgaggac ctctccacca cctcgggagg acagcaaagc tccaagagtg acagagtcgt
     1981 ggccagtaat gttaaagtag agactcagag tgatgaagag aatgggcgtg cctgtgaaat
     2041 gaatggggaa gaatgtgcgg aggatttacg aatgcttgat gcctcgggag agaaaatgaa
     2101 tggctcccac agggaccaag gcagctcggc tttgtcggga gttggaggca ttcgacttcc
     2161 taacggaaaa ctaaagtgtg atatctgtgg gatcatttgc atcgggccca atgtgctcat
     2221 ggttcacaaa agaagccaca ctggagaacg gcccttccag tgcaatcagt gcggggcctc
     2281 attcacccag aagggcaacc tgctccggca catcaagctg cattccgggg agaagccctt
     2341 caaatgccac ctctgcaact acgcctgccg ccggagggac gccctcactg gccacctgag
     2401 gacgcactcc gttggtaaac ctcacaaatg tggatattgt ggccgaagct ataaacagcg
     2461 aagctcttta gaggaacata aagagcgctg ccacaactac ttggaaagca tgggccttcc
     2521 gggcacactg tacccagtca ttaaagaaga aactaatcac agtgaaatgg cagaagacct
     2581 gtgcaagata ggatcagaga gatctctcgt gctggacaga ctagcaagta acgtcgccaa
     2641 acgtaagagc tctatgcctc agaaatttct tggggacaag ggcctgtccg acacgcccta
     2701 cgacagcagc gccagctacg agaaggagaa cgaaatgatg aagtcccacg tgatggacca
     2761 agccatcaac aacgccatca actacctggg ggccgagtcc ctgcgcccgc tggtgcagac
     2821 gcccccgggc ggttccgagg tggtcccggt catcagcccg atgtaccagc tgcacaagcc
     2881 gctcgcggag ggcaccccgc gctccaacca ctcggcccag gacagcgccg tggagaacct
     2941 gctgctgctc tccaaggcca agttggtgcc ctcggagcgc gaggcgtccc cgagcaacag
     3001 ctgccaagac tccacggaca ccgagagcaa caacgaggag cagcgcagcg gtctcatcta
     3061 cctgaccaac cacatcgccc cgcacgcgcg caacgggctg tcgctcaagg aggagcaccg
     3121 cgcctacgac ctgctgcgcg ccgcctccga gaactcgcag gacgcgctcc gcgtggtcag
     3181 caccagcggg gagcagatga aggtgtacaa gtgcgaacac tgccgggtgc tcttcctgga
     3241 tcacgtcatg tacaccatcc acatgggctg ccacggcttc cgtgatcctt ttgagtgcaa
     3301 catgtgcggc taccacagcc aggaccggta cgagttctcg tcgcacataa cgcgagggga
     3361 gcaccgcttc cacatgagct acccaacttt cttgtacaaa gtggtgggag gaggtggatc
     3421 tggcgactac aaggacgacg atgacaagtg actcgagcgg gatcaattcc gccccccccc
     3481 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3541 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3601 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3661 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3721 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3781 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3841 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3901 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3961 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4021 aacacgataa taccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4081 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4141 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4201 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4261 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4321 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4381 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4441 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4501 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4561 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4621 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4681 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4741 agctgtacaa gtaaagcggc cgcgactcta gagtcgacct gcaggcatgc aagcttcagg
     4801 tagccggcta acgttatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     4861 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4921 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4981 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5041 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5101 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5161 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5221 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5281 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5341 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5401 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     5461 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     5521 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     5581 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5641 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5821 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5881 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6061 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6121 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6241 ccaattcgcc ctatagtgag tcgtattagc atgtgagcaa aaggccagca taaatcaaaa
     6301 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6361 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6421 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6481 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6541 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6601 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     6661 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6721 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6781 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6901 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6961 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7021 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7081 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     7141 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     7201 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7261 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7321 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     7381 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7441 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7501 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7561 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7621 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7681 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     7741 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7801 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7861 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7921 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7981 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     8041 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8101 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     8161 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     8221 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     8281 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     8341 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     8401 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     8461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8701 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9241 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
     9301 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     9361 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     9421 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     9481 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     9541 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     9601 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     9661 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     9721 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     9781 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     9841 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9901 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9961 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10021 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10081 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10141 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10201 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10261 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10321 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10381 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10441 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10501 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10561 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10621 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10681 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10741 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10801 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10861 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10921 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10981 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11041 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11101 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11161 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11221 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    11281 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    11341 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    11401 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
    11461 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    11521 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    11581 aattttcggg tttattacag ggacagcaga gatccagttt g
//
