LOCUS       sequence-262550-       10078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for stable transfection of 
            eukaryotic cells with luciferse and hygromycin restistance. CMV 
            protomor drives SB100X transposase for transposon integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF112
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1134..1153
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1152..1207
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1253..1272
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2398..2454)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2464..4113)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      4110..4119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4741..5923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     5871..5891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5949..5958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6198..6215)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6204..6428
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6790..7093
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7094..7297
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7248..7268
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7294..7318
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          7435..7531
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     7588..7607
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7588..7606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7617..8639
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8656..8790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8680..8699)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8734..8753
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8943..9047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9048..9116,9117..9908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9266..9285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggg
     2401 gccaggattc tcctcgacgt caccagcctg cttcagcagg ctgaagttag tagctccact
     2461 gcccacggcg atcttgccgc ccttcttggc cttaatgaga atctcgcgga tcttgcgggc
     2521 gtccaacttg ccggtcagtc ctttaggcac ctcgtccacg aacacaacac caccgcgcag
     2581 cttcttggcg gttgtaacct ggctggccac atagtccacg atctccttct cggtcatggt
     2641 tttaccgtgt tccagcacga cgactgcggc gggcagctcg ccggcatcgt cgtcgggcag
     2701 gccggcgacc ccggcgtcga agatgttggg gtgttgcagc aggatgctct ccagttcggc
     2761 tggggctacc tggtagccct tgtatttgat caggctcttc agccggtcca cgatgaagaa
     2821 gtgctcgtcc tcgtcccagt aggcgatgtc gccgctgtgc agccagccgt ccttgtcgat
     2881 gagagcgttt gtagcctcgg ggttgttaac gtagccgctc atgatcatgg ggccacggac
     2941 gcacagctcg ccgcgctggt tcacacccag tgtcttaccg gtgtccaagt ccaccacctt
     3001 agcctcgaag aagggcacca ccttgcctac tgcgccaggc ttgtcgtccc cttcgggggt
     3061 gatcagaatg gcgctggttg tttctgtcag gccgtagccc tggcggatgc ctggtaggtg
     3121 gaagcgtttg gccacggcct cacctacctc cttgctgagc ggcgccccgc cgctggcgat
     3181 ctcgtgcaag ttgcttaggt cgtacttgtc gatgagagtg ctcttagcga agaagctaaa
     3241 tagtgtgggc accagcaggg cagattgaat cttatagtct tgcaagctgc gcaagaatag
     3301 ctcctcctcg aagcggtaca tgagcacgac ccgaaagccg cagatcaagt agcccagcgt
     3361 ggtgaacatg ccgaagccgt ggtgaaatgg caccacgctg aggatagcgg tgtcggggat
     3421 gatctggttg ccgaagatgg ggtcgcgggc atgactgaat cggacacaag cggtgcggtg
     3481 cggtagggct acgcccttgg gcaatccggt actgccacta ctgttcatga tcagggcgat
     3541 ggttttgtcc cggtcgaagc tctcgggcac gaagtcgtac tcgttgaagc cgggtggcaa
     3601 atgggaagtc acgaaggtgt acatgctttg gaagccctgg tagtcggtct tgctatccat
     3661 gatgatgatc ttttgtatga tcggtagctt cttttgcacg ttgaggatct tttgcagccc
     3721 tttcttgctc acgaatacga cggtgggctg gctgatgccc atgctgttca gcagctcgcg
     3781 ctcgttgtag atgtcgttag ctggggccac agccacaccg atgaacaggg cacccaacac
     3841 gggcatgaag aactgcaagc tattctcgct gcacaccacg atccgatggt ttgtattcag
     3901 cccatagcgc ttcatagctt ctgccagccg aacgctcatc tcgaagtact cggcgtaggt
     3961 aatgtccacc tcgatatgtg cgtcggtaaa ggcgatggtg ccgggcacca gggcgtagcg
     4021 cttcatggct ttgtgcagct gctcgccggc ggtcccgtct tcgagtgggt agaatggcgc
     4081 tgggcccttc ttaatgtttt tggcatcttc catggtggcg gtgcagaggt ttctacaggg
     4141 aaagagagaa aggctacagt tagtttgctg tgatgatgct aagtgccgag gtcagatgtc
     4201 tatggattgt acacggaatc ccaacgcagg aaccaaaaaa cactcaccca aaaaaatggc
     4261 gctaaggccg agagaaaggc gaagttccgt ctacggctat ataccttgaa acaggggcgg
     4321 cagaagtgac gcaacgcggt tgcgcggacg gcttcctgtg ttttcattgg tcgaagcgtt
     4381 ctgcgcgtgc gcagagattc cccagggaag gcgggccctc ttccgaatgt ccaatccgga
     4441 cattcttccg gttggacctc cgcggtcagc gctgtgcccg gcgagagatc acgtggggcg
     4501 cggaggcggt gctgctgggg cacggccgtc cagcctcggc ggccatattt ttgaggggct
     4561 gttcatctcg ttcacacgct ctgtccgcca tgtttgtgag tggaagcgcc attacccctt
     4621 caagcgactg aaggctgcag ggcctctggt ggcccgcatg gggagaccag acccgccagg
     4681 cccgcctttc cgcactcagt ccgggcttac tttattttgt gagacagggt ctcgcctaga
     4741 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4801 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4861 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4921 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     4981 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     5041 acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     5101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     5161 ttggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     5341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5761 gatggagttt ccccatactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5881 gacagtggtt caaagttttt ttcttccatt taaggtgtcg tgaaaactac cccaagctgg
     5941 cctctgaggc caccatggct cgagtttcga actcaattat actgaattgt ttcatcatag
     6001 ggttcaacat tgtgataaat caggacttca atgtctagtg ttcagtaaat accttgtagg
     6061 gctctccaaa aaggggaatc ttttcaggaa atgcctcttt ctcttggtgg gcttcctggt
     6121 tgcgtctttc cttctctcta attcgaaggc ctgtcaggcc aagcttgggg atcaattctc
     6181 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6241 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6301 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6361 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6421 ctctatggct tctgaggcgg aaagaaccag ctgggcctac tagatagcgg accccttacc
     6481 gaaacatcgc cgcattctgc agaggagtcg aacttagtgt atgtaaactt ctgacccact
     6541 ggaattgtga tacagtgaat tataagtgaa ataatctgtc tgtaaacaat tgttggaaaa
     6601 atgacttgtg tcatgcacaa agtagatgtc ctaactgact tgccaaaact attgtttgtt
     6661 aacaagaaat ttgtggagta gttgaaaaac gagttttaat gactccaact taagtgtatg
     6721 taaacttccg acttcaactg tatagggatc caattttaac aaaatattcg agcttgcatg
     6781 cctgcaggtc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6841 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6901 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6961 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     7021 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     7081 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     7141 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     7201 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     7261 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     7321 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     7381 ccggactcta gaggatccgg tactcgagga actgaaaaac cagaaagtta actggtaagt
     7441 ttagtctttt tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact
     7501 gctcctcagt ggatgttgcc tttacttcta ggcctgtacg gaagtgttac ttctgctcta
     7561 aaagctgcgg aattgtaccc gcggaattaa tacgactcac tatagggact agtaccatgg
     7621 gaaaatcaaa agaaatcagc caagacctca gaaaaagaat tgtagacctc cacaagtctg
     7681 gttcatcctt gggagcaatt tccaaacgcc tggcggtacc acgttcatct gtacaaacaa
     7741 tagtacgcaa gtataaacac catgggacca cgcagccgtc ataccgctca ggaaggagac
     7801 gcgttctgtc tcctagagat gaacgtactt tggtgcgaaa agtgcaaatc aatcccagaa
     7861 caacagcaaa ggaccttgtg aagatgctgg aggaaacagg tacaaaagta tctatatcca
     7921 cagtaaaacg agtcctatat cgacataacc tgaaaggcca ctcagcaagg aagaagccac
     7981 tgctccaaaa ccgacataag aaagccagac tacggtttgc aactgcacat ggggacaaag
     8041 atcgtacttt ttggagaaat gtcctctggt ctgatgaaac aaaaatagaa ctgtttggcc
     8101 ataatgacca tcgttatgtt tggaggaaga agggggaggc ttgcaagccg aagaacacca
     8161 tcccaaccgt gaagcacggg ggtggcagca tcatgttgtg ggggtgcttt gctgcaggag
     8221 ggactggtgc acttcacaaa atagatggca tcatggacgc ggtgcagtat gtggatatat
     8281 tgaagcaaca tctcaagaca tcagtcagga agttaaagct tggtcgcaaa tgggtcttcc
     8341 aacacgacaa tgaccccaag catacttcca aagttgtggc aaaatggctt aaggacaaca
     8401 aagtcaaggt attggagtgg ccatcacaaa gccctgacct caatcctata gaaaatttgt
     8461 gggcagaact gaaaaagcgt gtgcgagcaa ggaggcctac aaacctgact cagttacacc
     8521 agctctgtca ggaggaatgg gccaaaattc acccaaatta ttgtgggaag cttgtggaag
     8581 gctacccgaa acgtttgacc caagttaaac aatttaaagg caatgctacc aaatactagg
     8641 ggccctaacc gcggggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     8701 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8761 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     8821 caggttcagg gggaggtgtg ggaggttttt taacgcttac aatttcctga tgcggtattt
     8881 tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg
     8941 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9001 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9061 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9121 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9181 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9241 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9301 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9361 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9421 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9481 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9541 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9601 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9661 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9721 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9781 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9841 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9901 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9961 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10021 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
