LOCUS       sequence-262552-        5917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding Marburg virus VP30 gene under 
            control of the AG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger 
            E
  TITLE     A Chimeric Lloviu Virus Minigenome System Reveals that the 
            Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than
            Marburgviruses.
  JOURNAL   Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 
            10.1016/j.celrep.2018.08.008.
  PUBMED    30184492
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.08"; journalName: "Cell Rep"; date: 
            "2018-09-4- 4"; volume: "24"; issue: "10"; pages: "2573-2580.e4"
COMMENT     Sequence Label: pCAGGS_VP30_MARV
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2940..2959)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3005..3060
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3059..3078)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3420..3436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3420..3436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3433..3455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3444..3460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3468..3474,3475..3492,3493..3498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3513..3534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3593..3788
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3639..3774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3794..3928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3831..3850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3885..3904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3996..4013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4167..4755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4247..4266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4926..5717,5718..5786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5549..5568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5787..5891)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcaag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcagctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagggtacc
     1741 cccggggagc tcactagtgg atccaaaatg caacaacctc gcggaagaag tcgaacccgc
     1801 aaccaccaag tcacaccgac tatatatcat gaaactcaat tgccctccaa acctcattat
     1861 accaattatc atccacgtgc aagatcgatg agctcaaccc gtagtagtgc agaaagtagt
     1921 cccaccaatc atattccccg tgctcgacca ccctcaacat tcaacttatc gaaaccccct
     1981 cctcctccaa aagacatgtg caggaacatg aaaattggat tgccgtgcgc tgatcccact
     2041 tgtaatagag atcatgacct tgataatcta acaaatcgtg aacttttgct attgatggcc
     2101 cgaaaaatgc tccccaatac agacaagacc tttagaagtc cgcaggactg tggatcaccg
     2161 tctctttcta aaggtctctc aaaagataaa caggagcaaa cgaaagatgt gttgaccttg
     2221 gaaaatctag gacacattct gagctatctc cacagatcag aaattgggaa attggatgag
     2281 acatcacttc gtgcagcatt aagtctgacg tgtgctggaa ttcgaaagac gaatagatcc
     2341 ttgatcaaca ccatgacaga attacacatg aaccatgaaa atctcccgca agaccaaaac
     2401 ggtgttatca agcagaccta tacaggtatt caccttgaca aaggaggtca attcgaagcc
     2461 gccttatggc aaggttggga taagagatcg atatctctat tcgtacaagc agctttatat
     2521 gtaatgaaca atatcccctg tgaatcatca atcagtgtgc aagcctcata cgatcatttt
     2581 attcttcctc aaagtcaagg taaaggacag tgattattgt tcgaaagttg acaatttgat
     2641 cactttcagt tttcagtttc aacccttatc gcgagacttg aatacaatcc tactaacttc
     2701 aataagtgac cccaaattca agtttgctga aagctaagat gacaatgatc actagttcat
     2761 tgtaaattac tcgatcaaaa tgttcttaag ctatcttaag cttactgatg cggctctgct
     2821 tcacttttct tttgatttta aagccatagc tatatctaag tgtctaatta acaacttgta
     2881 cctctggatc cctgcagctc gagagggggg cggccgcgag ctcttaatta gctagcagat
     2941 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     3001 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     3061 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     3121 agtttggcaa catatgccca tatgctggct gccatgaaca aaggtggcta taaagaggtc
     3181 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     3241 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     3301 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     3361 ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc gtaatcatgg
     3421 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3481 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3541 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     3601 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3661 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3721 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3781 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3841 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3901 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     3961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     4381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5881 ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
