LOCUS       sequence-262554-        7040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding Marburg virus NP gene under control of the AG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E
  TITLE     Recombinant Marburg virus expressing EGFP allows rapid screening of 
            virus growth and real-time visualization of virus spread.
  JOURNAL   J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 
            10.1093/infdis/jir308.
  PUBMED    21987762
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Infect 
            Dis."; date: "2011-11"; volume: "204 Suppl 3:S861-70. doi"; pages: "
            10.1093/infdis/jir308"
COMMENT     Sequence Label: pCAGGS_NP_MARV
FEATURES             Location/Qualifiers
     source          1..7040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4063..4082)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4128..4183
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4182..4201)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4543..4559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4543..4559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4556..4578)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4567..4583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4591..4597,4598..4615,4616..4621))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4636..4657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4716..4911
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4762..4897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4917..5051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4954..4973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5008..5027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5119..5136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5290..5878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5370..5389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6049..6840,6841..6909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6672..6691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6910..7014)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcaag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcagctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcga agatatggat
     1741 ttacacagtt tgttggagtt gggtacaaaa cccactgccc ctcatgttcg taataagaaa
     1801 gtgatattat ttgacacaaa tcatcaggtt agtatctgta atcagataat agatgcaata
     1861 aactcaggga ttgatcttgg agatctccta gaagggggtt tgctgacgtt gtgtgttgag
     1921 cattactata attctgataa ggataaattc aacacaagtc ctatcgcgaa gtacttacgt
     1981 gatgcgggct atgaatttga tgtcatcaag aatgcagatg caacccgctt tctggatgtg
     2041 attcctaatg aacctcatta cagcccttta attctagccc ttaagacatt ggaaagtact
     2101 gaatctcaga gggggagaat tgggctcttt ttatcatttt gcagtctttt cctcccaaaa
     2161 cttgtcgtcg gagaccgagc tagtatcgaa aaggctttaa gacaagtaac agtgcatcaa
     2221 gaacagggga tcgtcacata ccctaatcat tggcttacca caggccacat gaaagtaatt
     2281 ttcgggattt tgaggtccag cttcatttta aagtttgtgt tgattcatca aggagtaaat
     2341 ttggtgacag gtcatgatgc ctatgacagt atcattagta attcagtagg tcaaactaga
     2401 ttctcaggac ttcttatcgt gaaaacagtt ctcgagttca tcttgcaaaa aactgattca
     2461 ggggtgacac tacatccttt ggtgcggacc tccaaagtaa aaaatgaagt tgctagtttc
     2521 aagcaggcgt tgagcaacct agcccgacat ggggaatacg caccatttgc acgggttctg
     2581 aatttatcag ggattaacaa cctcgaacat ggactctatc ctcagctttc agcaattgcg
     2641 ctgggtgtgg caacagcaca cggcagtaca ttggctggtg tcaatgttgg cgaacaatat
     2701 caacaactac gagaggcggc acatgatgcg gaagtaaaac tacaaaggcg acatgaacat
     2761 caggaaattc aagctattgc cgaggatgac gaggaaagga agatattaga acaattccac
     2821 cttcagaaaa ctgaaatcac acacagtcag acactagccg tcctcagcca gaaacgagaa
     2881 aaattagctc gtctcgctgc agaaattgaa aacaatattg tggaagatca gggatttaag
     2941 caatcacaga atcgggtgtc acagtcgttt ttgaatgacc ctacacctgt ggaagtaacg
     3001 gttcaagcca ggcccatgaa tcgaccaact gctctgcctc ccccagttga cgacaagatt
     3061 gagcatgaat ctacagaaga tagctcttct tcaagtagct ttgttgactt gaatgatcca
     3121 tttgcactgc tgaatgagga cgaggatact cttgatgaca gtgtcatgat cccgggcaca
     3181 acatcgagag aatttcaagg gattcctgaa ccgccaagac aatcccaaga cctcaataac
     3241 agccaaggaa agcaggaaga tgaatccaca aatccgatta agaaacagtt tctgagatat
     3301 caagaattgc ctcctgttca agaggatgat gaatcggaat acacaactga ctctcaagaa
     3361 agcatcgacc aaccaggatc cgacaatgaa caaggagttg atcttccacc tcctccgttg
     3421 tacgctcagg aaaaaagaca ggacccaata cagcacccag cagcaaaccc tcaggatccc
     3481 ttcggcagta ttggtgatgt aaatggtgat atcttagaac ctataagatc accttcttca
     3541 ccatctgctc ctcaggaaga cacaaggatg agggaagcct atgaattgtc gcctgatttc
     3601 acaaatgatg aggataatca gcagaattgg ccacaaagag tggtgacaaa gaagggtaga
     3661 actttccttt atcctaatga tcttctgcaa acaaatcctc cagagtcact tataacagcc
     3721 ctcgttgagg aataccaaaa tcctgtctca gctaaggagc ttcaagcaga ttggcccgac
     3781 atgtcatttg atgaaaggag acatgttgcg atgaacttgt agtccagata acacagcacg
     3841 gttactcact tatctatttt gatatgactc atcctcagat cacagcaatc aaatttattt
     3901 gaatatttga accacctttt agtatcctat tacttgttac tattgtgtga gacaacataa
     3961 gccatcaaat aacaatcacg ggcaaggact gggcatacta tggtggtcga attcctcgag
     4021 ggtacccccg gggcggccgc gagctcttaa ttagctagca gatctttttc cctctgccaa
     4081 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     4141 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     4201 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     4261 ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta tatgaaacag
     4321 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     4381 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     4441 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     4501 tgaagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     4561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4621 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4681 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     4741 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4861 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     4921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5041 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5461 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     5521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5701 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5821 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5881 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5941 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6001 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6061 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6121 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6181 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6241 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6301 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6361 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6421 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6481 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6541 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6601 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6661 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6721 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6781 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6841 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6901 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6961 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7021 tccccgaaaa gtgccacctg
//
