LOCUS       sequence-262564-       11588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IKZF3-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF3-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          110..1126
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1050..1072
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1134..1153
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1242..1266
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2794..2818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2838..2861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2896..3445
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3040..3057)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3267..3286
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3448..3450,3451..3453,3454..4167)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3448..3450,3451..3453,3454..4164)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3493..3514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3754..3773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4101..4122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4244..4832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4297..4317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4715..4726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4835..4851)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4836..4852)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5041..5274
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5346..5467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5383..5402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5437..5456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5507..5642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5662..5681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5663..5681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5704..6062)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5744..5765)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5956..5975
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6089..6193
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6195..6552
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6195..6386
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6218..6238)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6403..6538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6587..7381
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6587..7381
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6641..6660)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7251..7270
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7569..7588)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7613..7660
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7989..8577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8478..8497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8752..9055
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9057..9255
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9210..9230
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9273..9453
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9500..9625
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10122..10355
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10540..10584
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10882..10999
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        11097..11476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
       61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      301 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
      361 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
      421 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
      481 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
      541 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
      601 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
      661 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
      721 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
      781 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      841 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      901 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      961 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1021 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1081 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1141 gctggttatt gtgctgtctc atcattttgg caaagaattc ccctagccgc ggaatctaga
     1201 ctgccggatc gaattgtcga cgaattcgga tctggccaat cacaagtttg tacaaaaaag
     1261 ttggcatgga agatatacaa acaaatgcgg aactgaaaag cactcaggag cagtctgtgc
     1321 ccgcagaaag tgcagcggtt ttgaatgact acagtttaac caaatctcat gaaatggaaa
     1381 atgtggacag tggagaaggc ccagccaatg aagatgaaga cataggagat gattcaatga
     1441 aagtgaaaga tgaatacagt gaaagagatg agaatgtttt aaagtcagaa cccatgggaa
     1501 atgcagaaga gcctgaaatc ccttacagct attcaagaga atataatgaa tatgaaaaca
     1561 ttaagttgga gagacatgtt gtctcattcg atagtagcag gccaaccagt ggaaagatga
     1621 actgcgatgt gtgtggatta tcctgcatca gcttcaatgt cttaatggtt cataagcgaa
     1681 gccatactgg tgaacgccca ttccagtgta atcagtgtgg ggcatctttt actcagaaag
     1741 gtaacctcct ccgccacatt aaactgcaca caggggaaaa accttttaag tgtcacctct
     1801 gcaactatgc atgccaaaga agagatgcgc tcacggggca tcttaggaca cattctgtgg
     1861 agaaacccta caaatgtgag ttttgtggaa ggagttacaa gcagagaagt tcccttgagg
     1921 agcacaagga gcgctgccgt acatttcttc agagcactga cccaggggac actgcaagtg
     1981 cggaggcaag acacatcaaa gcagagatgg gaagtgaaag agctctcgta ctggacagat
     2041 tagcaagcaa tgtggcaaaa cgaaaaagct caatgcctca gaaattcatt ggtgagaagc
     2101 gccactgctt tgatgtcaac tataattcaa gttacatgta tgagaaagag agtgagctca
     2161 tacagacccg catgatggac caagccatca ataacgccat cagctatctt ggcgccgaag
     2221 ccctgcgccc cttggtccag acaccgcctg ctcccacctc ggagatggtt ccagttatca
     2281 gcagcatgta tcccatagcc ctcacccggg ctgagatgtc aaacggtgcc cctcaagagc
     2341 tggaaaagaa aagcatccac cttccagaga agagcgtgcc ttctgagaga ggcctctctc
     2401 ccaacaatag tggccacgac tccacggaca ctgacagcaa ccatgaagaa cgccagaatc
     2461 acatctatca gcaaaatcac atggtcctgt ctcgggcccg caatgggatg ccacttctga
     2521 aggaggttcc ccgctcttac gaactcctca agcccccgcc catctgccca agagactccg
     2581 tcaaagtgat caacaaggaa ggggaggtga tggatgtgta tcggtgtgac cactgccgcg
     2641 tcctcttcct ggactatgtg atgttcacga ttcacatggg ctgccacggc ttccgtgacc
     2701 ctttcgagtg taacatgtgt ggatatcgaa gccatgatcg gtatgagttc tcgtctcaca
     2761 tagccagagg agaacacaga gccctgctga agtgcccaac tttcttgtac aaagtggtgg
     2821 gaggaggtgg atctggcgac tacaaggacg acgatgacaa gtgactcgag cgggatcaat
     2881 tccgcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2941 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     3001 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     3061 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     3121 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     3181 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     3241 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3301 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     3361 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3421 ttttcctttg aaaaacacga taataccatg gtgagcaagg gcgaggagct gttcaccggg
     3481 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3541 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3601 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3661 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3721 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3781 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3841 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3901 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3961 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4021 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4081 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4141 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagagtcga cctgcaggca
     4201 tgcaagcttc aggtagccgg ctaacgttat caagcttatc gataatcaac ctctggatta
     4261 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4321 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4381 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4441 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4501 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4561 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4621 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     4681 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4741 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4801 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc
     4861 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4921 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4981 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5041 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5101 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5161 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5221 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     5281 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5341 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5461 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     5521 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5581 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5641 cctagggacg tacccaattc gccctatagt gagtcgtatt agcatgtgag caaaaggcca
     5701 gcataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5761 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5821 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5881 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5941 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     6001 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     6061 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6181 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     6241 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6301 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6361 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6541 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6601 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6661 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6721 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     6781 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6841 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6901 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6961 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7021 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7081 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     7141 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7201 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7261 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7321 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7381 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7441 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7501 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     7561 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     7621 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     7681 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     7741 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     7801 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     7861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8641 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
     8701 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     8761 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     8821 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     8881 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     8941 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     9001 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     9061 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     9121 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     9181 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     9241 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9301 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9361 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9421 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     9481 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     9541 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     9601 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     9661 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     9721 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     9781 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     9841 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     9901 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     9961 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    10021 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    10081 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    10141 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    10201 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    10261 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    10321 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    10381 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    10441 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    10501 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    10561 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    10621 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    10681 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    10741 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    10801 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
    10861 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    10921 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    10981 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttagt
    11041 accgggcccg ctctagccct agatttaaat cgatgtacgg gccagatata cgcgttgaca
    11101 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    11161 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    11221 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    11281 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    11341 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    11401 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    11461 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
    11521 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
    11581 ggcggggg
//
