LOCUS       sequence-262565-       11591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal expression of N-terminal intein-split prime editor v2 (PE2)
            with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used 
            by PE2, PE3, and PE3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, 
            Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
  TITLE     Search-and-replace genome editing without double-strand breaks or 
            donor DNA.
  JOURNAL   Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4.
  PUBMED    31634902
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4."
COMMENT     Sequence Label: U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH...
FEATURES             Location/Qualifiers
     source          1..11591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2623..2871
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2623..2643
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2794..2813
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2893..2968
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3007..3454
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3515..3535
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3536..5251
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5600..5620
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5630..5686
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5687..5689,5690..6400)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5687..5689,5690..6400)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5729..5750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5990..6009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6337..6358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6683..7271
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6736..6756)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7154..7165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7343..7576
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7602..7619)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7608..7832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7878..8306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7965..7984)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8175..8196
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8315..8335)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8320..8649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8500..8635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8562..8581
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8697..8744
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8763..9137
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9267..9388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9304..9323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9358..9377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9437..9453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9437..9453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9450..9472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9461..9477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9485..9491,9492..9509,9510..9515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9530..9551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9668..9685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9839..10427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9919..9938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10598..11389,11390..11458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11221..11240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11459..11563)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccaag gtcgggcagg aagagggcct atttcccatg
     2641 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     2701 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     2761 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     2821 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgcgggctgg
     2881 agctgttcgc gcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     2941 acttgaaaaa gtggcaccga gtcggtgcaa gatgcaagcg cgaacagctc cagttttttt
     3001 gaattcagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
     3061 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
     3121 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
     3181 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
     3241 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
     3301 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
     3361 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
     3421 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga atctagagcg ctgccaccat
     3481 gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacaa
     3541 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
     3601 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     3661 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     3721 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     3781 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     3841 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     3901 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     3961 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     4021 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     4081 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     4141 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     4201 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     4261 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     4321 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     4381 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     4441 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     4501 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     4561 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     4621 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     4681 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     4741 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     4801 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     4861 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     4921 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     4981 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     5041 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     5101 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     5161 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     5221 gctgaaagag gactacttca agaaaatcga gtgcctgtcc tacgagacag agatcctgac
     5281 agtggagtat ggcctgctgc caatcggcaa gatcgtggag aagaggatcg agtgtaccgt
     5341 gtactctgtg gataacaatg gcaacatcta tacacagccc gtggcacagt ggcacgatag
     5401 gggagagcag gaggtgttcg agtattgcct ggaggacggc agcctgatca gggcaaccaa
     5461 ggaccacaag ttcatgacag tggatggcca gatgctgccc atcgacgaga ttttcgagcg
     5521 ggagctggac ctgatgagag tggataacct gcctaatagc ggaggcagta aaagaacagc
     5581 agacgggagt gagtttgagc ccaagaaaaa gagaaaggtg ggatccggcg caacaaactt
     5641 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccggtga gcaagggcga
     5701 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5761 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5821 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5881 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5941 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6001 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6061 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6121 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6181 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6241 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6301 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6361 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgaga
     6421 ggaggaggag gagacagaca gcaggatgcc ccacctcgac agccccggca gctcccagcc
     6481 gagacgctcc ttcctctcaa gggtgatcag ggcagcgcta ccgttgcagc tgcttctgct
     6541 gctgctgctg ctcctggcct gcctgctacc tgcctctgaa gatgactaca gctgcaccca
     6601 ggccaacaac tttgcccgat ccttctaccc catgctgcgg tacaccaacg ggccacctcc
     6661 cacctaaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6721 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6781 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6841 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6901 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6961 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7021 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7081 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7141 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7201 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7261 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7321 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7381 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7441 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7501 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7561 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     7621 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7681 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7741 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7801 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7861 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8281 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     8341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8401 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8461 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8521 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8581 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8641 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     8701 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     8761 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     8821 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     8881 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     8941 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     9001 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     9061 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     9121 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     9181 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9241 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     9301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9361 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9421 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9481 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9541 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9601 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9721 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9781 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10021 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10261 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10381 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10441 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10501 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10561 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10621 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10681 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10741 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10801 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10861 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10921 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10981 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11041 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11101 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11161 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11221 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11281 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11341 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11401 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11461 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11521 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11581 tgccacctga c
//
