LOCUS       sequence-262566-       12746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal expression of C-terminal intein-split prime editor v2 
            (PE2). This plasmid is used by PE2, PE3, and PE3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, 
            Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
  TITLE     Search-and-replace genome editing without double-strand breaks or 
            donor DNA.
  JOURNAL   Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4.
  PUBMED    31634902
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4."
COMMENT     Sequence Label: hSynapsin-PE2-Cterminal-lenti
FEATURES             Location/Qualifiers
     source          1..12746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2608..3055
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3116..3136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3242..5626
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7799..7819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7838..8426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7891..7911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8309..8320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8498..8731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8757..8774)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8763..8987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9033..9461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9120..9139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9330..9351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9470..9490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9475..9804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9655..9790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9717..9736
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9852..9899
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9918..10292
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10422..10543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10459..10478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10513..10532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10592..10608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10592..10608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10605..10627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10616..10632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10640..10646,10647..10664,10665..10670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10685..10706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10823..10840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10994..11582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11074..11093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11753..12544,12545..12613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12376..12395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12614..12718)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccagt gcaagtgggt tttaggacca ggatgaggcg
     2641 gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat
     2701 tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc
     2761 gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg
     2821 ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc
     2881 ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc
     2941 gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc
     3001 gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag
     3061 aatctagagc gctgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
     3121 gaagaagcgg aaagtcatca agattgctac acggaaatac ctgggaaagc agaacgtgta
     3181 cgacatcggc gtggagcggg atcacaactt cgccctgaag aatggcttta tcgccagcaa
     3241 ttgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     3301 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     3361 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     3421 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     3481 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     3541 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     3601 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     3661 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     3721 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     3781 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     3841 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     3901 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     3961 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     4021 gtccgactac gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga
     4081 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     4141 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     4201 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     4261 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     4321 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     4381 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     4441 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     4501 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     4561 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     4621 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     4681 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     4741 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     4801 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     4861 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     4921 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     4981 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     5041 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     5101 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     5161 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     5221 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     5281 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     5341 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     5401 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     5461 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     5521 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     5581 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactctg gaggatctag
     5641 cggaggatcc tctggcagcg agacaccagg aacaagcgag tcagcaacac cagagagcag
     5701 tggcggcagc agcggcggca gcagcaccct aaatatagaa gatgagtatc ggctacatga
     5761 gacctcaaaa gagccagatg tttctctagg gtccacatgg ctgtctgatt ttcctcaggc
     5821 ctgggcggaa accgggggca tgggactggc agttcgccaa gctcctctga tcatacctct
     5881 gaaagcaacc tctacccccg tgtccataaa acaatacccc atgtcacaag aagccagact
     5941 ggggatcaag ccccacatac agagactgtt ggaccaggga atactggtac cctgccagtc
     6001 cccctggaac acgcccctgc tacccgttaa gaaaccaggg actaatgatt ataggcctgt
     6061 ccaggatctg agagaagtca acaagcgggt ggaagacatc caccccaccg tgcccaaccc
     6121 ttacaacctc ttgagcgggc tcccaccgtc ccaccagtgg tacactgtgc ttgatttaaa
     6181 ggatgccttt ttctgcctga gactccaccc caccagtcag cctctcttcg cctttgagtg
     6241 gagagatcca gagatgggaa tctcaggaca attgacctgg accagactcc cacagggttt
     6301 caaaaacagt cccaccctgt ttaatgaggc actgcacaga gacctagcag acttccggat
     6361 ccagcaccca gacttgatcc tgctacagta cgtggatgac ttactgctgg ccgccacttc
     6421 tgagctagac tgccaacaag gtactcgggc cctgttacaa accctaggga acctcgggta
     6481 tcgggcctcg gccaagaaag cccaaatttg ccagaaacag gtcaagtatc tggggtatct
     6541 tctaaaagag ggtcagagat ggctgactga ggccagaaaa gagactgtga tggggcagcc
     6601 tactccgaag acccctcgac aactaaggga gttcctaggg aaggcaggct tctgtcgcct
     6661 cttcatccct gggtttgcag aaatggcagc ccccctgtac cctctcacca aaccggggac
     6721 tctgtttaat tggggcccag accaacaaaa ggcctatcaa gaaatcaagc aagctcttct
     6781 aactgcccca gccctggggt tgccagattt gactaagccc tttgaactct ttgtcgacga
     6841 gaagcagggc tacgccaaag gtgtcctaac gcaaaaactg ggaccttggc gtcggccggt
     6901 ggcctacctg tccaaaaagc tagacccagt agcagctggg tggccccctt gcctacggat
     6961 ggtagcagcc attgccgtac tgacaaagga tgcaggcaag ctaaccatgg gacagccact
     7021 agtcattctg gccccccatg cagtagaggc actagtcaaa caaccccccg accgctggct
     7081 ttccaacgcc cggatgactc actatcaggc cttgcttttg gacacggacc gggtccagtt
     7141 cggaccggtg gtagccctga acccggctac gctgctccca ctgcctgagg aagggctgca
     7201 acacaactgc cttgatatcc tggccgaagc ccacggaacc cgacccgacc taacggacca
     7261 gccgctccca gacgccgacc acacctggta cacggatgga agcagtctct tacaagaggg
     7321 acagcgtaag gcgggagctg cggtgaccac cgagaccgag gtaatctggg ctaaagccct
     7381 gccagccggg acatccgctc agcgggctga actgatagca ctcacccagg ccctaaagat
     7441 ggcagaaggt aagaagctaa atgtttatac tgatagccgt tatgcttttg ctactgccca
     7501 tatccatgga gaaatataca gaaggcgtgg gtggctcaca tcagaaggca aagagatcaa
     7561 aaataaagac gagatcttgg ccctactaaa agccctcttt ctgcccaaaa gacttagcat
     7621 aatccattgt ccaggacatc aaaagggaca cagcgccgag gctagaggca accggatggc
     7681 tgaccaagcg gcccgaaagg cagccatcac agagactcca gacacctcta ccctcctcat
     7741 agaaaattca tcaccctctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc
     7801 caagaagaag aggaaagtct aaacgcgtta agtcgacaat caacctctgg attacaaaat
     7861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8221 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8341 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8401 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     8461 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8521 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8581 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8641 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8701 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     8761 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8821 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8881 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8941 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     9001 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     9061 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9121 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9181 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9241 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9301 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9361 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9421 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     9481 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9541 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9601 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9661 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9721 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     9781 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     9841 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     9901 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     9961 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
    10021 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
    10081 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
    10141 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
    10201 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
    10261 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
    10321 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10381 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    10561 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10621 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10681 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10741 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10801 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10861 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    11461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11701 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11761 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11821 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11881 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11941 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12001 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12061 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12121 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12181 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12241 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12301 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12361 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12421 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12481 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12541 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12601 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12661 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12721 catttccccg aaaagtgcca cctgac
//
