LOCUS       sequence-262601-        9273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of FLT3 ITD-Y591F/Y919F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Kazi JU, Sun J, Phung B, Zadjali F, Flores-Morales A, Ronnstrand L
  TITLE     Suppressor of cytokine signaling 6 (SOCS6) negatively regulates Flt3
            signal transduction through direct binding to phosphorylated 
            tyrosines 591 and 919 of Flt3.
  JOURNAL   J Biol Chem. 2012 Oct 19;287(43):36509-17. doi: 
            10.1074/jbc.M112.376111. Epub 2012 Sep 5.
  PUBMED    22952242
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.376111"; journalName: "J Biol Chem"; date: 
            "2012-10-19- 19"; volume: "287"; issue: "43"; pages: "36509-17"
COMMENT     Sequence Label: pMSCV-Flt3-ITD-Y591F/Y919F
FEATURES             Location/Qualifiers
     source          1..9273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             177..693
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    757..1098
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1165..1581
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1511..1533
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1549..1565
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        4625..5124
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4660..4683)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5057..5076
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5145..5744
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5145..5164)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5641..5661
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5844..6358
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6520..6536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6544..6550,6551..6568,6569..6574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6589..6610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6727..6744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6898..7486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6978..6997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7657..8448,8449..8517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8280..8299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8518..8622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8690..8708
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8746..8768)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8868..8887
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9081..9103
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9231..9250
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac
       61 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
      121 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
      181 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
      241 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
      301 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
      361 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc
      421 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
      481 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
      541 cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtat tcccaataaa
      601 gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga
      661 ttgattgact gcccacctcg ggggtctttc atttggaggt tccaccgaga tttggagacc
      721 cctgcccagg gaccaccgac ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg
      781 tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac
      841 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca
      901 cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc
      961 tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac
     1021 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc
     1081 cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1141 tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta
     1201 ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga
     1261 cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac
     1321 ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc acctggcccg
     1381 catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc
     1441 cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc
     1501 ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc
     1561 ctcactcctt ctctaggcgc cggaattcgt taaccatgcc ggcgttggcg cgcgacggcg
     1621 gccagctgcc gctgctcgtt gttttttctg caatgatatt tgggactatt acaaatcaag
     1681 atctgcctgt gatcaagtgt gttttaatca atcataagaa caatgattca tcagtgggga
     1741 agtcatcatc atatcccatg gtatcagaat ccccggaaga cctcgggtgt gcgttgagac
     1801 cccagagctc agggacagtg tacgaagctg ccgctgtgga agtggatgta tctgcttcca
     1861 tcacactgca agtgctggtc gatgccccag ggaacatttc ctgtctctgg gtctttaagc
     1921 acagctccct gaattgccag ccacattttg atttacaaaa cagaggagtt gtttccatgg
     1981 tcattttgaa aatgacagaa acccaagctg gagaatacct actttttatt cagagtgaag
     2041 ctaccaatta cacaatattg tttacagtga gtataagaaa taccctgctt tacacattaa
     2101 gaagacctta ctttagaaaa atggaaaacc aggacgccct ggtctgcata tctgagagcg
     2161 ttccagagcc gatcgtggaa tgggtgcttt gcgattcaca gggggaaagc tgtaaagaag
     2221 aaagtccagc tgttgttaaa aaggaggaaa aagtgcttca tgaattattt gggatggaca
     2281 taaggtgctg tgccagaaat gaactgggca gggaatgcac caggctgttc acaatagatc
     2341 taaatcaaac tcctcagacc acattgccac aattatttct taaagtaggg gaacccttat
     2401 ggataaggtg caaagctgtt catgtgaacc atggattcgg gctcacctgg gaattagaaa
     2461 acaaagcact cgaggagggc aactactttg agatgagtac ctattcaaca aacagaacta
     2521 tgatacggat tctgtttgct tttgtatcat cagtggcaag aaacgacacc ggatactaca
     2581 cttgttcctc ttcaaagcat cccagtcaat cagctttggt taccatcgta gaaaagggat
     2641 ttataaatgc taccaattca agtgaagatt atgaaattga ccaatatgaa gagttttgtt
     2701 tttctgtcag gtttaaagcc tacccacaaa tcagatgtac gtggaccttc tctcgaaaat
     2761 catttccttg tgagcaaaag ggtcttgata acggatacag catatccaag ttttgcaatc
     2821 ataagcacca gccaggagaa tatatattcc atgcagaaaa tgatgatgcc caatttacca
     2881 aaatgttcac gctgaatata agaaggaaac ctcaagtgct cgcagaagca tcggcaagtc
     2941 aggcgtcctg tttctcggat ggatacccat taccatcttg gacctggaag aagtgttcag
     3001 acaagtctcc caactgcaca gaagagatca cagaaggagt ctggaataga aaggctaaca
     3061 gaaaagtgtt tggacagtgg gtgtcgagca gtactctaaa catgagtgaa gccataaaag
     3121 ggttcctggt caagtgctgt gcatacaatt cccttggcac atcttgtgag acgatccttt
     3181 taaactctcc aggccccttc cctttcatcc aagacaacat ctcattctat gcaacaattg
     3241 gtgtttgtct cctcttcatt gtcgttttaa ccctgctaat ttgtcacaag tacaaaaagc
     3301 aatttaggta tgaaagccag ctacagatgg tacaggtgac cggctcctca gataatgagt
     3361 acttcttcgt tgatttcaga gaatatgaat atgatctcag agaatatgaa tatgatctca
     3421 aatgggagtt tccaagagaa aatttagagt ttgggaaggt actaggatca ggtgcttttg
     3481 gaaaagtgat gaacgcaaca gcttatggaa ttagcaaaac aggagtctca atccaggttg
     3541 ccgtcaaaat gctgaaagaa aaagcagaca gctctgaaag agaggcactc atgtcagaac
     3601 tcaagatgat gacccagctg ggaagccacg agaatattgt gaacctgctg ggggcgtgca
     3661 cactgtcagg accaatttac ttgatttttg aatactgttg ctatggtgat cttctcaact
     3721 atctaagaag taaaagagaa aaatttcaca ggacttggac agagattttc aaggaacaca
     3781 atttcagttt ttaccccact ttccaatcac atccaaattc cagcatgcct ggttcaagag
     3841 aagttcagat acacccggac tcggatcaaa tctcagggct tcatgggaat tcatttcact
     3901 ctgaagatga aattgaatat gaaaaccaaa aaaggctgga agaagaggag gacttgaatg
     3961 tgcttacatt tgaagatctt ctttgctttg catatcaagt tgccaaagga atggaatttc
     4021 tggaatttaa gtcgtgtgtt cacagagacc tggccgccag gaacgtgctt gtcacccacg
     4081 ggaaagtggt gaagatatgt gactttggat tggctcgaga tatcatgagt gattccaact
     4141 atgttgtcag gggcaatgcc cgtctgcctg taaaatggat ggcccccgaa agcctgtttg
     4201 aaggcatcta caccattaag agtgatgtct ggtcatatgg aatattactg tgggaaatct
     4261 tctcacttgg tgtgaatcct taccctggca ttccggttga tgctaacttc tacaaactga
     4321 ttcaaaatgg atttaaaatg gatcagccat tttatgctac agaagaaata ttcattataa
     4381 tgcaatcctg ctgggctttt gactcaagga aacggccatc cttccctaat ttgacttcgt
     4441 ttttaggatg tcagctggca gatgcagaag aagcgatgta tcagaatgtg gatggccgtg
     4501 tttcggaatg tcctcacacc taccaaaaca ggcgaccttt cagcagagag atggatttgg
     4561 ggctactctc tccgcaggct caggtcgaag attcgtagtt aacctcgaga gatctaattc
     4621 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     4681 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     4741 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     4801 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     4861 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     4921 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     4981 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     5041 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     5101 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     5161 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5221 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5281 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5341 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5401 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5461 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5521 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5581 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5641 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5701 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     5761 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat
     5821 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     5881 aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc
     5941 aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt
     6001 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag
     6061 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     6121 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     6181 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc
     6241 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     6301 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg
     6361 gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa
     6421 tcctgactca attagccact gttttgaatc cacatactcc aatactcctg aaatagttca
     6481 ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc tcacaattcc
     6541 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6601 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6721 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6781 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8641 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8701 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     8761 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     8821 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     8881 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     8941 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     9001 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     9061 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc aaggaatggt
     9121 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     9181 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     9241 gcgatatagg cgccagcaac cgcacctgtg gcg
//
