LOCUS       sequence-262619-        9234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast reporter gene with estrogen response element (ERE) upstream of
            CYC1 promoter driving lacZ expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Picard D, Khursheed B, Garabedian MJ, Fortin MG, Lindquist S, 
            Yamamoto KR
  TITLE     Reduced levels of hsp90 compromise steroid receptor action in vivo.
  JOURNAL   Nature 1990 Nov 8;348(6297):166-8.
  PUBMED    2234079
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "1990-11-8"; volume: "348(6297)"; pages: "166-8"
COMMENT     Sequence Label: pUCdeltaSS-ERE
FEATURES             Location/Qualifiers
     source          1..9234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2169..3511
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    2501..2548
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        4103..4318
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4319..5122
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8833..8849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8833..8849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8846..8868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8857..8873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8881..8887,8888..8905,8906..8911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8926..8947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9064..9081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc ttcaagaatt ctgaaccagt cctaaaacga gtaaatagga
     1861 ccggcaattc ttcaagcaat aaacaggaat accaattatt aaaagataac ttagtcagat
     1921 cgtacaataa agctttgaag aaaaatgcgc cttattcaat ctttgctata aaaaatggcc
     1981 caaaatctca cattggaaga catttgatga cctcatttct ttcaatgaag ggcctaacgg
     2041 agttgactaa tgttgtggga aattggagcg ataagcgtgc ttctgccgtg gccaggacaa
     2101 cgtatactca tcagataaca gcaatacctg atcactactt cgcactagtt tctcggtact
     2161 atgcatatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
     2221 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
     2281 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
     2341 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
     2401 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
     2461 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
     2521 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
     2581 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
     2641 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
     2701 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
     2761 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
     2821 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
     2881 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
     2941 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
     3001 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
     3061 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     3121 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     3181 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     3241 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     3301 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     3361 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     3421 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     3481 gttctacaaa atgaagcaca gatgcttcgt taacaaagat atgctattga agtgcaagat
     3541 ggaaacgcag aaaatgaacc ggggatgcga cgtgcaagat tacctatgca atagatgcaa
     3601 tagtttctcc aggaaccgaa atacatacat tgtcttccgt aaagcgctag actatatatt
     3661 attatacagg ttcaaatata ctatctgttt cagggaaaac tcccaggttc ggatgttcaa
     3721 aattcaatga tgggtaacaa gtacgatcgt aaatctgtaa aacagtttgt cggatattag
     3781 gctgtatctc ctcaaagcgt attcgaatat cattgagaag ctgcagcgtc acatcggata
     3841 ataatgatgg cagccattgt agaagtgcct tttgcatttc tagtctcttt ctcggtctag
     3901 ctagttttac tacatcgcga agatagaatc ttagatcaca ctgcctttgc tgagctggat
     3961 caatagagta acaaaagagt ggtaaggcct cgttaaagga caaggacctg agcggaagtg
     4021 tatcgtacag tagacggagt atactagtat agtctatagt ccgtggaatt ctcatgtttg
     4081 acagcttatc atcgataagc ttttcaattc atcatttttt ttttattctt ttttttgatt
     4141 tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
     4201 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
     4261 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
     4321 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct
     4381 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac
     4441 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca
     4501 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc
     4561 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt
     4621 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc
     4681 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac
     4741 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac
     4801 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg
     4861 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac
     4921 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga
     4981 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg
     5041 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag
     5101 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc
     5161 acaaattaga gcttcaattt aattatatca gttattaccc tcgacctcga ctttgatcag
     5221 gtcactgtga cctgactcga gtcaggtcac agtgacctga tcaaagtcga gcagatccgc
     5281 caggcgtgta tatagcgtgg atggccaggc aactttagtg ctgacacata caggcatata
     5341 tatatgtgtg cgacgacaca tgatcatatg gcatgcatgt gctctgtatg tatataaaac
     5401 tcttgttttc ttcttttctc taaatattct ttccttatac attaggtcct ttgtagcata
     5461 aattactata cttctataga cacgcaaaca caaatacaca cactaaatta ataatgaccg
     5521 gatccggagc ttggctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     5581 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5641 tttcccgact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5701 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg
     5761 cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg
     5821 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca
     5881 ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg
     5941 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
     6001 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
     6061 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
     6121 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg
     6181 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac
     6241 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg
     6301 aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat
     6361 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
     6421 agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt
     6481 ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca
     6541 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc
     6601 tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc
     6661 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
     6721 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc
     6781 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
     6841 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa
     6901 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
     6961 aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt
     7021 cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc
     7081 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
     7141 aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
     7201 cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc
     7261 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
     7321 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt
     7381 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa
     7441 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg
     7501 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta
     7561 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg
     7621 aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag
     7681 tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc
     7741 gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga
     7801 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
     7861 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
     7921 gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
     7981 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag
     8041 cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg
     8101 cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta
     8161 gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc
     8221 ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
     8281 ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc
     8341 cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
     8401 cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
     8461 gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
     8521 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
     8581 cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt
     8641 gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
     8701 ttgtactgcc ggaccaccga ctgtgagcca ctccggccat ggggcggaga atgggcggaa
     8761 ctgggcggag ttaggggcgg gatgggcgga gttaggggcc tctaggggta ccgagctcga
     8821 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtt
//
