LOCUS       sequence-262647-        7718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Calcium channel beta1b auxillary subunit in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Page KM, Stephens GJ, Berrow NS, Dolphin AC
  TITLE     The intracellular loop between domains I and II of the B-type 
            calcium channel confers aspects of G-protein sensitivity to the 
            E-type calcium channel.
  JOURNAL   J Neurosci. 1997 Feb 15;17(4):1330-8.
  PUBMED    9006976
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1997-02-15"; volume: "17(4)"; pages: "1330-8"
COMMENT     Sequence Label: cacnb1b (rat) in pMT2 vector
FEATURES             Location/Qualifiers
     source          1..7718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          379..608
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     591..610
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3347..3910
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    4097..4231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4134..4153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4188..4207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4904..4921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5075..5663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5155..5174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5834..6625,6626..6694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6457..6476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6695..6799)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6867..6885
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6923..6945)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7045..7064
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7258..7280
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7272..7289
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7273..7289
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7299..7628)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7313..7448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7613..7633
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtccaggcca gcacgaagga ggctaagtgg gaggggtagc ggtcgttgtc cactaggggg
       61 tccactcgct ccagggtgtg aagacacatg tcgccctctt cggcatcaag gaaggtgatt
      121 ggtttatagg tgtaggccac gtgaccgggt gttcctgaag gggggctata aaagggggtg
      181 ggggcgcgtt cgtcctcact ctcttccgca tcgctgtctg cgagggccag ctgttgggct
      241 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      301 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      361 aacctctcga ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa
      421 gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt
      481 tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt
      541 gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct
      601 ctccacaggt gtccactccc aggtccaact gcaggcgagc ctgaattcgg cacgagagcg
      661 tgcagctggg cgactgcacc cggtgctggc tgcgcgacgc cgcgctgctc tcggctcgga
      721 cggcctctcc catgcgctga gggcccccgg ctgtgccggg agggcggccg gaccggagga
      781 tcctctccat ggtccagaag agcggcatgt cccggggccc ttacccacct tcccaagaga
      841 tccctatgga ggtcttcgac cccagcccac agggcaaata cagcaagagg aaagggcggt
      901 tcaaaaggtc agatgggagc acctcctcag ataccacatc caacagcttt gtccgtcagg
      961 gctcagcaga gtcctacacg agccggccgt cagactccga cgtgtccctg gaggaggacc
     1021 gggaagcctt aaggaaggag gcagagcgcc aggccttagc ccagctcgag aaagccaaga
     1081 ccaaaccagt ggcctttgct gttcggacaa atgttggcta caatccgtct ccaggagatg
     1141 aggtgccagt gcagggagtg gccatcacct ttgagcccaa ggacttcctg cacatcaagg
     1201 agaaatacaa taatgactgg tggatcggga ggctggtgaa ggaaggctgt gaggttggtt
     1261 tcatccccag ccctgtcaaa ctggacagcc ttcgcctgct gcaggaacag acgctgcgcc
     1321 aaaaccgcct cagctccagc aagtcaggtg acaactccag ttccagtctg ggagatgtgg
     1381 tgactggcac ccgccgcccc acaccccccg ccagtgccaa acagaagcag aagtcgacag
     1441 agcacgtgcc cccctatgac gtggtgcctt ccatgaggcc catcatcctg gtgggaccat
     1501 cgctcaaggg ctatgaggta actgacatga tgcagaaggc gttgtttgac ttcttgaagc
     1561 atcggtttga tggcaggatt tccatcaccc gggtaacggc tgacatttcc ctggccaagc
     1621 gctcggtcct caacaacccc agcaaacaca ttatcatcga gcgctccaac actcgctcca
     1681 gcctggctga ggtacagagt gaaatcgagc ggatcttcga gctggcccgg accttgcagc
     1741 tggttgccct ggacgccgac accatcaacc acccagccca gctctctaaa acctcgctgg
     1801 cccccatcat tgtttacatc aagatcacat cccccaaggt actgcagagg ctcatcaaat
     1861 cccgagggaa gtctcaatcc aaacacctca atgtccaaat agcagcctcg gagaagctgg
     1921 cacagtgtcc ccccgaaatg tttgacataa tcctggacga gaaccaattg gaagatgcct
     1981 gcgagcacct ggcggagtac ttggaagcct actggaaggc cacacacccg cctagcagca
     2041 cgccacccaa tccgctgctg aaccgcacca tggctaccgc cgctctggct gccagccctg
     2101 cccccgtctc caacctccag ggaccctacc ttgcttccgg ggaccagccg ctggaccggg
     2161 ccactgggga gcatgccagt gtgcacgagt accccgggga gctgggccag cccccaggcc
     2221 tttaccccag caaccaccca cctggccggg caggcaccct gcgggcgcta tcccgccaag
     2281 acacctttga tgctgacacc cccggcagcc gaaactctgc ctacacggag ccaggagact
     2341 cgtgtgtgga catggagaca gacccctcag agggcccagg gcctggagac cctgcagggg
     2401 gaggtacacc accagctcgg cagggctcct gggaagagga ggaagattat gaggaggaga
     2461 tgaccgacaa caggaaccgg ggccggaata aggcccgcta ctgtgcggag ggtggtgggc
     2521 cggttctggg gcgcaataag aatgagctgg agggctgggg acaaggcgtc tacatccgct
     2581 gagaggcagg ggcagttctg ggagagaaga ggctctgaac tcggggaggg gagggcagag
     2641 gggcgcacca cccaagatgt gtcttgcctc cagggggcgc tttgtccctc attttcagat
     2701 gcctttgctc aaagcttggg gtttctttgg tggttatcat tcccaactcc tgggaggtcc
     2761 ttaatcccca gctgtaggtt tttccctaaa ctgctgtggg tggatggggg aatacctact
     2821 ttctgaagtg ccccctttcc ccatctttgg ggctttctcc ctcccagaga aaaggtgcac
     2881 ttccttaact ttttactcgg ggctgtaagc ggaggtccta attggggtgg ggtggagggg
     2941 tttcttctac caacgcaggg gtacttggga aaggttgggt gcttttcctg aagagaaccc
     3001 gctgaatctt ccacaaaccc cagacccggg atcccccacc atatccattc cataacctag
     3061 tttcctagca gcattctctg gtgggtgaaa gccacagctt ggaaacccat gaagagcttg
     3121 ctttggggag cagagctggg tgcaaactga cgggcatcag tgtgtgctct ctgccctcca
     3181 tggtcatggc aagaaagcag ggcctagaaa cttgaggagt gagcaactca agatgctgcc
     3241 tcgtgccgaa ttccgggggg ggggacagct cagggctgcg atttcgcgcc aaacttgacg
     3301 gcaatcctag cgtgaaggct ggtaggattt tatccccgct gccatcatgg ttcgaccatt
     3361 gaactgcatc gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg
     3421 gcctccgctc aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga
     3481 aggtaaacag aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa
     3541 tcgaccttta aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg
     3601 aggagctcat tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga
     3661 attggcaagt aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc
     3721 catgaatcaa ccaggccacc tcagactctt tgtgacaagg atcatgcagg aatttgaaag
     3781 tgacacgttt ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg
     3841 cgtcctctct gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa
     3901 gaaagactaa caggaagatg ctttcaagtt ctctgctccc ctcctaaagc tatgcatttt
     3961 tataagacca tgggactttt gctggcttta gatcataatc agccatacca catttgtaga
     4021 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4081 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4201 actcatcaat gtatcttatc atgtctggat ccccggccaa cggtctggtg acccggctgc
     4261 gagagctcgg tgtacctgag acgcgagtaa gcccttgagt caaagacgta gtcgttgcaa
     4321 gtccgcacca ggtactgata tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc
     4381 cagcgtaggg tggccggggc tccgggggcg aggtcttcca acataaggcg atgatatccg
     4441 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg
     4501 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg
     4561 ccggtgaggc gtgcgcagtc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg
     4621 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt
     4681 tcgaaccccg gatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc
     4741 caggtgtgcg acgtcagaca acgggggagc gctccttttg gcttccttcc aggcgcggcg
     4801 gctgctgcgc tagctttttt ggcgagctcg aattaattct gcattaatga atcggccaac
     4861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6121 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6421 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6841 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     6901 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6961 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7021 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7081 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     7141 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     7201 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     7261 agtcacgacg ttgtaaaacg acggccagtg ccaagctttt tgcaaaagcc taggcctcca
     7321 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     7381 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     7441 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     7501 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     7561 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     7621 tgacacacat tccacaggat ccggtcgcgc gaatttcgag cggtgttccg cggtcctcct
     7681 cgtatagaaa ctcggaccac tctgagacga aggctcgc
//
