LOCUS       sequence-262649-        5664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS driven variant of iGluSnFR for zebrafish expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA
  TITLE     Presynaptic Inhibition Selectively Gates Auditory Transmission to 
            the Brainstem Startle Circuit.
  JOURNAL   Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 
            10.1016/j.cub.2018.06.020. Epub 2018 Aug 2.
  PUBMED    30078569
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2018.06.020"; journalName: "Curr Biol"; date: 
            "2018-08-20- 20"; volume: "28"; issue: "16"; pages: "2527-2535.e8"
COMMENT     Sequence Label: UAS:iGluSnfr.zf1
FEATURES             Location/Qualifiers
     source          1..5664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..34
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    151..172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(187..192,193..210,211..217)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    225..241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     230..252
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     249..265
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     249..265
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1949..1970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2076..2097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2337..2356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2562..2591
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2595..2738
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKP"
     primer_bind     complement(3313..3330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3313..3329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3322..3344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3538..3557)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3657..3679
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3717..3735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3803..3907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3908..3976,3977..4768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4126..4145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4939..5527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5428..5447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
       61 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      121 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      181 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      241 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatggt ccctttagcc
      301 agtagcggtt ctaggcacgg gccgtccggg cggtggcctg gggcggaaaa ctgaaggggg
      361 gcggcaccgg cggctcagcc cttgtaatat attatatgca ccactattgg tttacttatg
      421 tcacagtttg taagtttgta acagcctgaa cctggatcag cttaggcctc caaggcggag
      481 tactgtcctc cgggctggcg gagtactgtc ctccggcaag gtcggagtac tgtcctccga
      541 cactagaggt cggagtactg tcctccgacg caaggcggag tactgtcctc cgggctgcgg
      601 agtactgtcc tccggcaagg tcggagtact gtcctccgac actagaggtc ggagtactgt
      661 cctccgacgc aaggtcggag tactgtcctc cgacactaga ggtcggagta ctgtcctccg
      721 acgcaaggtc ggagtactgt cctccgacac tagaggtcgg agtactgtcc tccgacgcaa
      781 ggcggagtac tgtcctccgg gctggcggag tactgtcctc cggcaagggt cgactctaga
      841 gggtatataa tggatcccat cgcgtctcag cctcactttg agctcctcca cacgaattcg
      901 ctagcgccac catgttcgct ctgggaatct atctgtggga aactatcgtg ttcttcagcc
      961 tggctgccag ccagcaggct gctagaagcg ccgcaggcag cacgctggac aaaatcgcca
     1021 aaaacggtgt gattgtcgtc ggtcaccgtg aatcttcagt gcctttctct tattacgaca
     1081 atcagcaaaa agtggtgggt tactcgcagg attactccaa cgccattgtt gaagcagtga
     1141 aaaagaaact caacaaaccg gacttgcagg taaaactgat tccgattacc tcacaaaacc
     1201 gtattccact gctgcaaaac ggcactttcg attttgaatg tggttctacc accaacaacg
     1261 tcgaacgcca aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt
     1321 tgaccaaaaa gggtggcgat atcaaagatt ttgccaacct gaaagacaaa gccgtagtcg
     1381 tcacttccgg cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga
     1441 atatgcgcat catcagcgcc aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc
     1501 gtgccgttgc ctttatgatg gatgacgtgc tgctggccgg tgaacgtgcg aaagcgaaga
     1561 aaccagacaa ctgggaaatc gtcggcaagc cgcagtctca ggaggcctac ggttgtatgt
     1621 tgcgtaaaga tgatccgcag ttcaaaaagc tgatggatga caccatcgct caggtgcaga
     1681 cctccggtga agcggaaaaa tggtttgata agtggttcaa aaatccaatt ctggtgagcc
     1741 acaacgtcta tatcatggcc gacaagcaga ggaacggcat caaggcgaac ttcaagatcc
     1801 gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca
     1861 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaaacttt
     1921 cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1981 ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagca
     2041 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2101 acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga
     2161 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2221 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2281 tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg
     2341 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2401 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcat aagctggagt
     2461 acaactttaa caaccccctg aacatgaatt tcgaactgtc agacgaaatg aaagcactgt
     2521 tcaaagaacc gaatgacaag gcactgaaac tgcaggtcga cgaacaaaaa ctcatctcag
     2581 aagaggatct gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct
     2641 tgccctttaa ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct
     2701 cccttatcat cctcatcatg ctttggcaga agaagccacc tagtaattaa gtctcagcca
     2761 ccgttaactg aacatgtcaa aacctgtgga gactgttgag atttgatgtt ctgaaaagat
     2821 aaagcctata aataaaatgt tgcccaaatt tcctgcctga tgtttttctt tgtctttgct
     2881 acatggcttt gctgctcgga tcggctcact ctgtgtatgc cacgttcact ttgtactctc
     2941 cttctcacgg taggtttatt atttttagat gtgcagttag tttctgtgaa ataacacacc
     3001 acacactgat attgtctgtg cattgacttg gtgagtgcac attgtttttg atcttgacat
     3061 atttatattt gattgatcag gtgaactgtg tgaatctaaa gtgctccata cagatgttct
     3121 gcattgaaaa tattctcatt ttattagtgg aagtgagtgt atgccagcgg ccgcggtacc
     3181 ctgcagtcta gacatttcag ttgacgaaga caaacaaagt tctgttgtga ctatgggggg
     3241 ggggggcgcc tggggatggt ctcgcccggg gagtaattca gggtagaacc gccactgcct
     3301 ttagcgacat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3361 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3421 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     3481 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3541 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     3601 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     3661 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     3721 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4081 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4321 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4381 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4441 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4501 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4561 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4621 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4681 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4741 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5041 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5641 gtgagctgat accgctcgcc gcag
//
