LOCUS       sequence-262655-       11084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pCAG driving expression of multicistronic Lyn-tagged 
            iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and 
            Histone 2B fused to mScarlet in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0419d
FEATURES             Location/Qualifiers
     source          1..11084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      124..712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     613..632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     824..843
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        824..842
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   872..906
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1446..1749
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1751..2025
                     /label=chicken beta-actin promoter
     intron          2026..3042
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2966..2988
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3050..3069
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4123..4179
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4204..4230
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4237..4908
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             4237..4908
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             4939..4995
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5206..5208,5209..5211,5212..5922)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5251..5272)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5512..5531)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5859..5880
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5953..6009
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6031..6408
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(7282..7301)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7347..7402
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7401..7420)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        8306..8509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8460..8480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8531..8540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8537..9565
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             8537..9565
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(9577..9594)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9583..9790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9997..10059
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10110..10128)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(10168..10983)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10168..10983)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10891..10910
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10975..10994
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat
       61 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      781 tgcgttatcc cctgattctg tggataaccg tgcggccgcc aattaatacg actcactata
      841 gggcgaattg gggcgcgcca ttctagatta accctagaaa gatagtctgc gtaaaattga
      901 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
      961 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
     1021 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
     1081 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
     1141 tgataactta ttatatatat attttcttgt tatagatatc aactagaatg ctagcatggg
     1201 cccagatctc catgggccct gaattcgcat ctagactgaa ctggccgata attgcagacg
     1261 aggagcatcg cccttccccg gccctcaggt aagaggacca aataccgtag ccgtttccaa
     1321 tttcagtcct ttagcgccac ctggtgctaa ctactctatc acgcttttat ccaataacta
     1381 cctttgtaaa tttcctttca aaagttctgg ccgggcgcgg tggcaacgcc atatatggag
     1441 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1501 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1561 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1621 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1681 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1741 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     1801 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     1861 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
     1921 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
     1981 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
     2041 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     2101 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     2161 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
     2221 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
     2281 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     2341 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     2401 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     2461 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     2521 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     2581 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     2641 ggggagggct cgggggaggg gcgcggcggc ccccggagtg ccggcggctg tcgaggcgcg
     2701 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     2761 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     2821 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     2881 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     2941 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     3001 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     3061 gttattgtgc tgtctcatca ttttggcaaa gaattcgcca ctatgggctg tatcaagagc
     3121 aagaggaagg acgtcgagaa cggttctgct gaaggttctg ttgctagaca accagacttg
     3181 ttgacttgtg acgacgaacc aatccacatc ccaggtgcta tccaaccaca cggtttgttg
     3241 ttggctttgg ctgctgacat gactatcgtt gctggttctg acaacttgcc agaattgact
     3301 ggtttggcta tcggtgcttt gatcggtaga tctgctgctg acgttttcga ctctgaaact
     3361 cacaacagat tgactatcgc tttggctgaa ccaggtgctg ctgttggtgc tccaatcact
     3421 gttggtttca ctatgagaaa ggacgctggt ttcatcggtt cttggcacag acacgaccaa
     3481 ttgatcttct tggaattgga accaccacaa agagatgttg ctgaaccaca agctttcttc
     3541 agaagaacta actctgctat cagaagattg caagctgctg aaactttgga atctgcttgt
     3601 gctgctgctg ctcaagaagt tagaaagatc actggtttcg acagagttat gatctacaga
     3661 ttcgcttctg acttctctgg tgaagttatc gctgaagaca gatgtgctga agttgaatct
     3721 aagttgggtt tgcactaccc agcttctact gttccagctc aagctagaag attgtacact
     3781 atcaacccag ttagaatcat cccagacatc aactacagac cagttccagt tactccagac
     3841 ttgaacccag ttactggtag accaatcgac ttgtctttcg ctatcttgag atctgtttct
     3901 ccagttcact tggaattcat gagaaacatc ggtatgcacg gtactatgtc tatctctatc
     3961 ttgagaggtg aaagattgtg gggtttgatc gtttgtcacc acagaactcc atactacgtt
     4021 gacttggacg gtagacaagc ttgtgaattg gttgctcaag ttttggcttg gcaaatcggt
     4081 gttatggaag aaggatccgg tgggctgcca aggggttctg gagctactaa ttttagcctt
     4141 ttgaaacaag ctggtgatgt tgaagagaat cctggtcccg gaacgggcgg aggaggtagt
     4201 atgctgcccc ccctggagag gctgaccctg ggttctgtga gcgtgatcaa gcccgagatg
     4261 aagatcaagc tgtgcatgag gggcaccgtg aacggccaca acttcgtgat cgagggcgag
     4321 ggcaagggca acccctacga gggcacccag atcctggacc tgaacgtgac cgagggcgcc
     4381 cccctgccct tcgcctacga catcctgacc accgtgttcc agtacggcaa cagggccttc
     4441 accaagtacc ccgccgacat ccaggactac ttcaagcaga ccttccccga gggctaccac
     4501 tgggagagga gcatgaccta cgaggaccag ggcatctgca ccgccaccag caacatcagc
     4561 atgaggggcg actgcttctt ctacgacatc aggttcgacg gcaccaactt cccccccaac
     4621 ggccccgtga tgcagaagaa gaccctgaag tgggagccca gcaccgagaa gatgtacgtg
     4681 gaggacggcg tgctgaaggg cgacgtgaac atgaggctgc tgctggaggg cggcggccac
     4741 tacaggtgcg acttcaagac cacctacaag gccaagaagg aggtgaggct gcccgacgcc
     4801 cacaagatcg accacaggat cgagatcctg aagcacgaca aggactacaa caaggtgaag
     4861 ctgtacgaga acgccgtggc caggtactcc atgctgccca gccaggccgg atccggtggg
     4921 ctggatgggg gttccggggc caccaacttc tccctcctta agcaggccgg cgacgtcgag
     4981 gaaaaccccg gcccaggaac gggcggagga ggtagtatgc gtaagccaga tctccgcgtt
     5041 cttatcccac ctggcagcaa gaacacgatg ccatcagtga atcaaaggat aaataactcc
     5101 aagcgctcgg ccggactcaa cgaagacgag tcgccatcta agaagctggc gtcgccggag
     5161 gtgtcgtcaa ggctggagcg cctgaccctc cagtccagca ctagtatggt gagcaagggc
     5221 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5281 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5341 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5401 agctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     5461 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5521 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5581 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5641 gccatccacg gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     5701 ttcggcctga actgcaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5761 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5821 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5881 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccgg tgggctggtt
     5941 cggggatcag gtgcaacaaa tttctcacta ctcaaacagg caggagatgt agaagaaaat
     6001 ccaggaccgg gaacgggcgg aggaggtagt atgccagagc cagcgaagtc tgctcccgcc
     6061 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
     6121 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac
     6181 cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt
     6241 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc
     6301 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac
     6361 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaaggg ttctgtatca
     6421 aaaggcgagg cggtcattaa agagttcatg aggtttaaag tccatatgga aggaagcatg
     6481 aatggtcacg agtttgagat agagggagag ggtgagggaa ggccgtacga agggacgcaa
     6541 actgcgaagt tgaaggtaac gaaaggcggc cctctgccat tctcttggga catcctttca
     6601 ccgcaattta tgtacggctc tagggccttc acaaaacacc ccgccgatat tccggattac
     6661 tataaacaaa gttttcctga gggcttcaag tgggaaagag taatgaactt cgaggatggg
     6721 ggagctgtga cggttactca agacactagc ctggaggacg gtactcttat atacaaggta
     6781 aaactaagag ggacgaattt tccgccagac ggccccgtga tgcaaaaaaa aactatgggg
     6841 tgggaagcat ccaccgagag gctgtacccc gaagacgggg tcctgaaggg tgatattaaa
     6901 atggcattaa gattaaaaga tggcgggaga tacttagctg acttcaagac tacatataaa
     6961 gccaaaaaac ctgtacagat gcctggagct tacaatgtgg atcgtaagct ggatattaca
     7021 tcccacaacg aagattatac cgtggtggag cagtacgagc gttcagaggg gcgtcactcc
     7081 accgggggta tggatgaatt atacaagagc ggtcttagaa gcagggcgca agcgtcaaat
     7141 tcagcggtag atggaacagc aggtccaggt agtactggca gcagaggatc ctaactcgag
     7201 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     7261 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     7321 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     7381 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     7441 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     7501 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     7561 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     7621 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     7681 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     7741 gcccaagctt ggcgtaatgc tgagcagtta cagagatgtt acgaaccctg aacaggttga
     7801 ctattaattg tgtctgcttg atgtggacac caggtggcgc tggacatcag atttggagag
     7861 gcagttgtct agggaaccgg gctctgtgcc agcgcaggag gcaggctggc tctcctattc
     7921 cagggatgct catccaggaa ggaaaggttg catgctggac acactaacct tacagcggtg
     7981 ggctctatgg ggtacctacg taggtacata acttacggta aatggcccgc ctggctgacc
     8041 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     8101 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     8161 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     8221 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     8281 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     8341 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     8401 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     8461 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     8521 ccgtcagata gccaccatgg gtaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     8581 tctgatcgaa aagttcgaca gcgtgtccga cctgatgcag ctctcggagg gcgaagaatc
     8641 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     8701 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     8761 tccggaagtg cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg
     8821 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcaacc
     8881 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     8941 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     9001 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     9061 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     9121 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     9181 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     9241 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     9301 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     9361 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     9421 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     9481 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     9541 cactcgtccg agggcaaagg aataactcga gcgacgcctc gactgtgcct tctagttgcc
     9601 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     9661 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     9721 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     9781 atgctgggga tgcggtgggc tctatgagta tcgatactag ttaaaagttt tgttacttta
     9841 tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt
     9901 tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat
     9961 taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt
    10021 atctttaacg tacgtcacaa tatgattatc tttctagggt taatctagta tacgcgtatg
    10081 cggccgctta attaatccag cttttgttcc ctttagtgag ggttaattcc gagcggccgc
    10141 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
    10201 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
    10261 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
    10321 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
    10381 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat
    10441 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
    10501 aaccaaaccg ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt
    10561 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
    10621 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
    10681 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
    10741 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
    10801 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
    10861 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
    10921 agcatccatg ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct
    10981 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
    11041 atttttatct tgtgcaatgt aacatcagag attttgagac acaa
//
