LOCUS       sequence-26266-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag CAMKV
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..89
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    144..168
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccctcnntc cttctctagg cgccggccgg atccagaatt cgccaccatg gggtcttcaa
       61 aatctaaacc aaaggacccc agccagcgcc ggcgcaggat ccgaggttac cttgactaca
      121 aagacgatga cgacaagcaa ttgacaagtt tgtacaaaaa agttggcatg ccgtttgggt
      181 gtgtgactct gggcgacaag aagaactata accagccatc ggaggtgact gacagatatg
      241 atttgggaca ggtcatcaag actgaggagt tttgtgaaat cttccgggcc aaggacaaga
      301 cgacaggcaa gctgcacacc tgcaagaagt tccagaagcg ggacggccgc aaggtgcgga
      361 aagctgccaa gaacgagata ggcatcctca agatggtgaa gcatcccaac atcctacagc
      421 tggtggatgt gtttgtgacc cgcaaggagt actttatctt cctggagctg gccacgggga
      481 gggaggtgtt tgactggatc ctggaccagg gctactactc ggagcgagac acaagcaacg
      541 tggtacggca agtcctggag gccgtggcct atttgcactc actcaagatc gtgcacagga
      601 atctcaagct ggagaacctg gtttactaca accggctgaa gaactcgaag attgtcatca
      661 gtgacttcca tctggctaag ctagaaaatg gcctcatcaa ggagccctgt gggacccccg
      721 agtatctggc cccagangtg gtangccgg
//
