LOCUS       sequence-262675-       17442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pCAG driving expression of multicistronic Lyn-tagged 
            iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and 
            Histone 2B fused to mScarlet in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17442)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 17442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0419v
FEATURES             Location/Qualifiers
     source          1..17442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      61..649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     550..569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..780
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        761..779
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   809..843
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1383..1686
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1688..1964
                     /label=chicken beta-actin promoter
     intron          1965..2981
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     polyA_signal    4058..4282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4533..4836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4838..5114
                     /label=chicken beta-actin promoter
     intron          5115..6131
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             6195..6221
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             6228..6899
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             6228..6899
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     primer_bind     complement(6939..6958)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6993..7012
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        7282..7585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7587..7863
                     /label=chicken beta-actin promoter
     intron          7864..8880
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             join(9130..9132,9133..9135,9136..9846)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9175..9196)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9436..9455)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9783..9804
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10008..10063
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     enhancer        10667..10970
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10972..11248
                     /label=chicken beta-actin promoter
     intron          11249..12265
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             12326..12703
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     polyA_signal    13642..13697
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        14601..14804
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14755..14775
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      14826..14835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14832..15860
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             14832..15860
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    15878..16085
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   16292..16354
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(16405..16423)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(16463..17278)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(16463..17278)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     17186..17205
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     17270..17289
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
       61 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      181 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      721 gttatcccct gattctgtgg ataaccgtgc ggccgccaat taatacgact cactataggg
      781 cgaattgggg cgcgccattc tagattaacc ctagaaagat agtctgcgta aaattgacgc
      841 atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt
      901 aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt
      961 cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt
     1021 gacttttaag atttaactca tacgataatt atattgttat ttcatgttct acttacgtga
     1081 taacttatta tatatatatt ttcttgttat agatatcaac tagaatgcta gcatgggccc
     1141 agatctccat gggccctgaa ttcgcatcta gactgaactg gccgataatt gcagacgagg
     1201 agcatcgccc ttccccggcc ctcaggtaag aggaccaaat accgtagccg tttccaattt
     1261 cagtccttta gcgccacctg gtgctaacta ctctatcacg cttttatcca ataactacct
     1321 ttgtaaattt cctttcaaaa gttctggccg ggcgcggtgg caacgccata tatggagttc
     1381 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1441 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1501 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1561 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1621 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1681 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     1741 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     1801 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     1861 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     1921 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     1981 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     2041 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     2101 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     2161 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     2221 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     2281 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     2341 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     2401 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     2461 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     2521 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     2581 gggagggctc gggggagggg cgcggcggcc cccggagtgc cggcggctgt cgaggcgcgg
     2641 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     2701 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     2761 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     2821 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     2881 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2941 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     3001 ttattgtgct gtctcatcat tttggcaaag aattcgccac tatgggctgt atcaagagca
     3061 agaggaagga cgtcgagaac ggttctgctg aaggttctgt tgctagacaa ccagacttgt
     3121 tgacttgtga cgacgaacca atccacatcc caggtgctat ccaaccacac ggtttgttgt
     3181 tggctttggc tgctgacatg actatcgttg ctggttctga caacttgcca gaattgactg
     3241 gtttggctat cggtgctttg atcggtagat ctgctgctga cgttttcgac tctgaaactc
     3301 acaacagatt gactatcgct ttggctgaac caggtgctgc tgttggtgct ccaatcactg
     3361 ttggtttcac tatgagaaag gacgctggtt tcatcggttc ttggcacaga cacgaccaat
     3421 tgatcttctt ggaattggaa ccaccacaaa gagatgttgc tgaaccacaa gctttcttca
     3481 gaagaactaa ctctgctatc agaagattgc aagctgctga aactttggaa tctgcttgtg
     3541 ctgctgctgc tcaagaagtt agaaagatca ctggtttcga cagagttatg atctacagat
     3601 tcgcttctga cttctctggt gaagttatcg ctgaagacag atgtgctgaa gttgaatcta
     3661 agttgggttt gcactaccca gcttctactg ttccagctca agctagaaga ttgtacacta
     3721 tcaacccagt tagaatcatc ccagacatca actacagacc agttccagtt actccagact
     3781 tgaacccagt tactggtaga ccaatcgact tgtctttcgc tatcttgaga tctgtttctc
     3841 cagttcactt ggaattcatg agaaacatcg gtatgcacgg tactatgtct atctctatct
     3901 tgagaggtga aagattgtgg ggtttgatcg tttgtcacca cagaactcca tactacgttg
     3961 acttggacgg tagacaagct tgtgaattgg ttgctcaagt tttggcttgg caaatcggtg
     4021 ttatggaaga aggatcctaa ctcgagcgac gcctcgactg tgccttctag ttgccagcca
     4081 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4141 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4201 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4261 ggggatgcgg tgggctctat gggctgccaa accagatgtc aacacagcta cggtctgccg
     4321 cttcaggtcc atttacggaa gccgcaatcc tgcactatga ccaccagggg gagcgctagg
     4381 cccatccctg cacaggtagc tatttttaga ggttgcttct agcctggagg aagaaacgaa
     4441 agaaataaaa gactatgata cttttttttt ttaaccggac caacttataa attatttgta
     4501 agtccaataa aaacgccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     4561 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4621 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4681 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4741 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4801 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     4861 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     4921 ttattttgtg cagcgatggg ggcggggggg gggggggggg sgcgccaggc ggggcggggc
     4981 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     5041 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     5101 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     5161 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     5221 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     5281 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     5341 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     5401 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     5461 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     5521 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     5581 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     5641 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     5701 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     5761 cccggagtgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     5821 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     5881 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     5941 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     6001 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     6061 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     6121 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     6181 aattcgccac tatgctgccc cccctggaga ggctgaccct gggttctgtg agcgtgatca
     6241 agcccgagat gaagatcaag ctgtgcatga ggggcaccgt gaacggccac aacttcgtga
     6301 tcgagggcga gggcaagggc aacccctacg agggcaccca gatcctggac ctgaacgtga
     6361 ccgagggcgc ccccctgccc ttcgcctacg acatcctgac caccgtgttc cagtacggca
     6421 acagggcctt caccaagtac cccgccgaca tccaggacta cttcaagcag accttccccg
     6481 agggctacca ctgggagagg agcatgacct acgaggacca gggcatctgc accgccacca
     6541 gcaacatcag catgaggggc gactgcttct tctacgacat caggttcgac ggcaccaact
     6601 tcccccccaa cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga
     6661 agatgtacgt ggaggacggc gtgctgaagg gcgacgtgaa catgaggctg ctgctggagg
     6721 gcggcggcca ctacaggtgc gacttcaaga ccacctacaa ggccaagaag gaggtgaggc
     6781 tgcccgacgc ccacaagatc gaccacagga tcgagatcct gaagcacgac aaggactaca
     6841 acaaggtgaa gctgtacgag aacgccgtgg ccaggtactc catgctgccc agccaggccg
     6901 gatcctaact cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     6961 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     7021 taaccattat aagctgcaat aaacgctgga tgcacacact ggcttaagat gacgggaccc
     7081 tgtaaacgtg tccgccttgc cactttgtgc aatatttagc tccttcagta gagggcgctg
     7141 caggaacatg tcaggagggg gccttctctt ccggattctg gattctggta tacacttgga
     7201 gcgtggcatt tgggggacag ccactggtgc gcagccccat caagttttgg tggcatccct
     7261 aacgccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     7321 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     7381 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     7441 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     7501 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     7561 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     7621 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     7681 agcgatgggg gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     7741 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     7801 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     7861 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     7921 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     7981 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     8041 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     8101 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     8161 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     8221 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     8281 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     8341 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     8401 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     8461 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagtgcc
     8521 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     8581 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     8641 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     8701 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     8761 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     8821 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     8881 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccact
     8941 atgcgtaagc cagatctccg cgttcttatc ccacctggca gcaagaacac gatgccatca
     9001 gtgaatcaaa ggataaataa ctccaagcgc tcggccggac tcaacgaaga cgagtcgcca
     9061 tctaagaagc tggcgtcgcc ggaggtgtcg tcaaggctgg agcgcctgac cctccagtcc
     9121 agcactagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     9181 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     9241 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     9301 tggcccaccc tcgtgaccac cctgagctgg ggcgtgcagt gcttcgcccg ctaccccgac
     9361 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     9421 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     9481 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     9541 ctggggcaca agctggagta caacgccatc cacggcaacg tctatatcac cgccgacaag
     9601 cagaagaacg gcatcaaggc caacttcggc ctgaactgca acatcgagga cggcagcgtg
     9661 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     9721 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     9781 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     9841 tacaagggat cctaactcga gattcactcc tcaggtgcag gctgcctatc agaaggtggt
     9901 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     9961 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
    10021 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
    10081 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
    10141 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
    10201 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
    10261 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
    10321 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
    10381 atggagatcc ctcgacctgc agcccaagct tggcgtaatg ctggttctgt atgggcacag
    10441 acaacctaac taatttcctg gccctcaagg tgacttgctt gaacttgcca ccttcgccac
    10501 tagggggcag cattggttta cacagggtga acaaacgttc aattctaaac taatattctt
    10561 tggtgggaaa gtgtgttcat tttggtttgt tttgaagaag tcacttcata cctttgaact
    10621 ggggttaagc tggttaactc ccaaaaacgc catatatgga gttccgcgtt acataactta
    10681 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    10741 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    10801 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10861 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10921 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
    10981 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
    11041 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcgcc
    11101 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
    11161 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
    11221 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
    11281 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
    11341 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
    11401 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
    11461 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
    11521 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
    11581 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
    11641 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
    11701 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
    11761 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
    11821 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
    11881 ggggcgcggc ggcccccgga gygccggcgg ctgtcgaggc gcggcgagcc gcagccattg
    11941 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
    12001 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
    12061 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
    12121 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
    12181 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
    12241 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
    12301 tcattttggc aaagaattcg ccactatgcc agagccagcg aagtctgctc ccgccccgaa
    12361 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
    12421 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
    12481 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga
    12541 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
    12601 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
    12661 gtccgagggt actaaggcca tcaccaagta caccagcgct aagggttctg tatcaaaagg
    12721 cgaggcggtc attaaagagt tcatgaggtt taaagtccat atggaaggaa gcatgaatgg
    12781 tcacgagttt gagatagagg gagagggtga gggaaggccg tacgaaggga cgcaaactgc
    12841 gaagttgaag gtaacgaaag gcggccctct gccattctct tgggacatcc tttcaccgca
    12901 atttatgtac ggctctaggg ccttcacaaa acaccccgcc gatattccgg attactataa
    12961 acaaagtttt cctgagggct tcaagtggga aagagtaatg aacttcgagg atgggggagc
    13021 tgtgacggtt actcaagaca ctagcctgga ggacggtact cttatataca aggtaaaact
    13081 aagagggacg aattttccgc cagacggccc cgtgatgcaa aaaaaaacta tggggtggga
    13141 agcatccacc gagaggctgt accccgaaga cggggtcctg aagggtgata ttaaaatggc
    13201 attaagatta aaagatggcg ggagatactt agctgacttc aagactacat ataaagccaa
    13261 aaaacctgta cagatgcctg gagcttacaa tgtggatcgt aagctggata ttacatccca
    13321 caacgaagat tataccgtgg tggagcagta cgagcgttca gaggggcgtc actccaccgg
    13381 gggtatggat gaattataca agagcggtct tagaagcagg gcgcaagcgt caaattcagc
    13441 ggtagatgga acagcaggtc caggtagtac tggcagcaga ggatcctaac tcgagattca
    13501 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
    13561 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
    13621 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
    13681 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
    13741 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
    13801 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
    13861 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
    13921 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
    13981 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
    14041 agcttggcgt aatgctgagc agttacagag atgttacgaa ccctgaacag gttgactatt
    14101 aattgtgtct gcttgatgtg gacaccaggt ggcgctggac atcagatttg gagaggcagt
    14161 tgtctaggga accgggctct gtgccagcgc aggaggcagg ctggctctcc tattccaggg
    14221 atgctcatcc aggaaggaaa ggttgcatgc tggacacact aaccttacag cggtgggctc
    14281 tatggggtac ctacgtaggt acataactta cggtaaatgg cccgcctggc tgaccgccca
    14341 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    14401 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
    14461 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
    14521 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    14581 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
    14641 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
    14701 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
    14761 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
    14821 agatagccac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
    14881 tcgaaaagtt cgacagcgtg tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
    14941 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
    15001 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
    15061 aagtgcttga cattggggaa tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac
    15121 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg caaccggtcg
    15181 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
    15241 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
    15301 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
    15361 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
    15421 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
    15481 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
    15541 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
    15601 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
    15661 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
    15721 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
    15781 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
    15841 gtccgagggc aaaggaataa ctcgagcgac gcctcgactg tgccttctag ttgccagcca
    15901 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
    15961 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
    16021 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
    16081 ggggatgcgg tgggctctat gagtatcgat actagttaaa agttttgtta ctttatagaa
    16141 gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg
    16201 aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata
    16261 aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt
    16321 taacgtacgt cacaatatga ttatctttct agggttaatc tagtatacgc gtatgcggcc
    16381 gcttaattaa tccagctttt gttcccttta gtgagggtta attccgagcg gccgcgtgtt
    16441 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
    16501 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
    16561 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
    16621 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
    16681 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt
    16741 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
    16801 aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag
    16861 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
    16921 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
    16981 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
    17041 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
    17101 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
    17161 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
    17221 ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata tggctcataa
    17281 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
    17341 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc
    17401 gaacttttgc tgagttgaag gatcagtcat gaccaaaatc cc
//
