LOCUS       sequence-262678-       11980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged H2-Ld.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-H2-Ld-VN
FEATURES             Location/Qualifiers
     source          1..11980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1129..1131,1132..1590)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(1171..1192)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1432..1451)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    1613..1747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1637..1656)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1691..1710
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1889..1911)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2497..4286
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4592..6517)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6952..6970)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7038..7142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7143..7211,7212..8003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7361..7380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8174..8762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8663..8682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9379..9524
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9564..10589
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9564..10589
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10610..10629)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10654..10702
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11355..11734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11735..11938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11889..11909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11935..11959
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcagttc aggaggcgga
      121 ccacattcta tgcgttattt cgagaccgcc gtgtcccggc ccggcctcgg ggagccccgg
      181 tacatctctg tcggctatgt ggacaacaag gagttcgtgc gcttcgacag cgacgcggag
      241 aatccgagat atgagccgca ggcgccgtgg atggagcagg aggggccgga gtattgggag
      301 cggatcacgc agatcgccaa gggccaggag cagtggttcc gagtgaacct gaggaccctg
      361 ctcggctact acaaccagag cgcgggcggc actcacacac tccagtggat gtacggctgt
      421 gacgtggggt cggacgggcg cctcctccgc gggtacgagc agttcgccta cgacggctgc
      481 gattacatcg ccctgaacga agacctgaaa acgtggacgg cggcggacat ggcggcgcag
      541 atcacccgac gcaagtggga gcaggctggt gctgcagagt attacagggc ctacctggag
      601 ggcgagtgcg tggagtggct ccacagatac ctgaagaacg ggaacgcgac gctgctgcgc
      661 acagattccc caaaggcaca tgtgacccat caccccagat ctaaaggtga agtcaccctg
      721 aggtgctggg ccctgggctt ctaccctgct gacatcaccc tgacctggca gttgaatggg
      781 gaggagctga cccaggacat ggagcttgtg gagaccaggc ctgcagggga tggaaccttc
      841 cagaagtggg catctgtggt ggtgcctctt gggaaggagc agaattacac atgccgtgtg
      901 taccatgagg ggctgcctga gcccctcacc ctgagatggg agcctcctcc atctacggac
      961 agctacatgg tcatagtagc tgttcttggt gtgctcgggg caatggcaat cattggtgca
     1021 gtggtagcgt ttgtgatgaa acgccgacga aataccggag gtaaaggtgg cgattatgcc
     1081 ttggcaccag ggagccagtc atcagagatg tcactgcgcg actgcaaggt gagcaagggc
     1141 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1201 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1261 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1321 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1381 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1441 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1501 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1561 tacaacagcc acaacgtcta tctgaccgcc taactcgagg ccggcaaggc cggatccaga
     1621 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1681 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1741 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtggggag
     1801 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc
     1861 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     1921 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     1981 ttggcgggtg tcggggcgca gccatgaccg gtcgactcta gagccccgcc gccggacgaa
     2041 ctaaacctga ctacggcatc tctgcccctt cttcgctggt acgaggagcg cttttgtttt
     2101 gtattcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     2161 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     2221 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     2281 tttgcatgct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     2341 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     2401 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     2461 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     2521 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     2581 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     2641 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     2701 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     2761 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     2821 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     2881 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     2941 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     3001 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     3061 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     3121 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     3181 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     3241 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     3301 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     3361 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     3421 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     3481 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     3541 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     3601 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     3661 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     3721 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     3781 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     3841 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     3901 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     3961 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     4021 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     4081 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     4141 tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata
     4201 cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg
     4261 aattttcgct gcttgtcctt ttcctgcatg ctggttgctc ccattcttag gtgaatttaa
     4321 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
     4381 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
     4441 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
     4501 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
     4561 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
     4621 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
     4681 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
     4741 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
     4801 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
     4861 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
     4921 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
     4981 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
     5041 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
     5101 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
     5161 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
     5221 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
     5281 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
     5341 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
     5401 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
     5461 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
     5521 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg
     5581 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     5641 ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5701 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     5761 cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     5821 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg
     5881 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     5941 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     6001 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     6061 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     6121 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
     6181 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
     6241 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
     6301 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
     6361 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
     6421 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
     6481 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
     6541 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
     6601 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
     6661 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
     6721 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
     6781 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
     6841 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     6901 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
     6961 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7381 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7681 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8281 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctgcgcc gcgtgcggct
     8821 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     8881 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     8941 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     9001 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     9061 gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc
     9121 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     9181 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     9241 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccctgc
     9301 ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg
     9361 catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg
     9421 aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca cttcgcatat taaggtgacg
     9481 cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg
     9541 ccgcagatcc cgggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     9601 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     9661 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     9721 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     9781 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     9841 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     9901 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     9961 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
    10021 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
    10081 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
    10141 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
    10201 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
    10261 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
    10321 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
    10381 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    10441 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    10501 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    10561 ccagcactcg tccgagggca aaggaatagg ggagatgggg gaggctaact gaaacacgga
    10621 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
    10681 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
    10741 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
    10801 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
    10861 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
    10921 tcgtgggggt tattattttg ggcgttgcgt ggggtctggt ccacgactgg actgagcaga
    10981 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
    11041 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
    11101 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    11161 cgttgttgca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
    11221 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
    11281 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
    11341 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
    11401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    11461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    11521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    11581 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
    11641 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
    11701 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
    11761 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
    11821 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
    11881 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
    11941 tttagtgaac cgtcagatct ctagaagctg ggtaccagct
//
