LOCUS       sequence-262698-        4686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO1C1
FEATURES             Location/Qualifiers
     source          1..4686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2811..2910)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2927..2946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2928..2946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2951..2967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2951..2967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3069..3088)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3080..3889
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3147..3166)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4036..4624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4525..4544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggata ccagctccaa ggagaacatc cagctgttct gcaagacaag
      721 cgtgcagcca gtgggccgcc cttcctttaa gaccgagtac ccctctagcg aggagaagca
      781 gccttgctgt ggcgagctga aggtgttcct gtgcgccctg agcttcgtgt actttgccaa
      841 ggccctggcc gagggctatc tgaagtccac catcacacag atcgagcgga gatttgacat
      901 cccatcctct ctggtgggcg tgatcgatgg ctctttcgag atcggcaatc tgctggtcat
      961 caccttcgtg agctattttg gcgccaagct gcacaggcca aagatcatcg gagcaggatg
     1021 cgtgatcatg ggagtgggca cactgctgat cgccatgcct cagttcttta tggagcagta
     1081 caagtatgag cggtacagcc ctagctccaa ctccaccctg agcatctccc catgcctgct
     1141 ggagtctagc tcccagctgc ccgtgtctgt gatggagaag tctaagagca agatcagcaa
     1201 tgagtgtgag gtggatacat ctagctccat gtggatctac gtgttcctgg gcaacctgct
     1261 gagaggcatc ggcgagaccc caatccagcc cctgggcatc gcctatctgg acgatttcgc
     1321 cagcgaggac aatgccgcct tttacatcgg ctgcgtgcag accgtggcca tcatcggccc
     1381 catcttcggc tttctgctgg gctccctgtg cgccaagctg tacgtggaca tcggctttgt
     1441 gaacctggat cacatcacca tcacacctaa ggacccacag tgggtgggag catggtggct
     1501 gggctatctg atcgcaggaa tcatctccct gctggcagca gtgccctttt ggtacctgcc
     1561 aaagtctctg ccccggtccc agtctagaga ggactccaat tctagctccg agaagtctaa
     1621 gttcatcatc gacgatcaca ccgattatca gacacctcag ggcgagaacg ccaagatcat
     1681 ggagatggcc agggactttc tgccaagcct gaagaacctg ttcggcaatc ccgtgtactt
     1741 tctgtatctg tgcacctcta cagtgcagtt caatagcctg tttggcatgg tgacctacaa
     1801 gccaaagtat atcgagcagc agtacggcca gtctagctcc cgcgccaatt tcgtgatcgg
     1861 cctgatcaac atcccagcag tggccctggg aatcttctcc ggcggcatcg tgatgaagaa
     1921 gtttcggatc agcgtgtgcg gagcagcaaa gctgtacctg ggatctagcg tgttcggcta
     1981 tctgctgttc ctgtccctgt ttgccctggg ctgtgagaac tctgatgtgg caggactgac
     2041 cgtgagctac cagggaacaa agcccgtgag ctatcacgag agagccctgt ttagcgactg
     2101 caattccagg tgcaagtgta gcgagacaaa gtgggagcca atgtgcggcg agaacggcat
     2161 cacctacgtg agcgcctgcc tggcaggatg tcagacatct aacagaagcg gcaagaatat
     2221 catcttctac aactgcacct gcgtgggaat cgcagcaagc aagtccggca attcctctgg
     2281 catcgtgggc aggtgccaga aggataacgg ctgtcctcag atgttcctgt acttcctggt
     2341 catctctgtg atcaccagct atacactgtc cctgggcggc atccctggct acatcctgct
     2401 gctgagatgc atcaagccac agctgaagag cttcgccctg ggcatctata cactggccat
     2461 cagggtgctg gcaggaatcc cagcacccgt gtacttcggc gtgctgatcg ataccagctg
     2521 tctgaagtgg ggctttaaga ggtgcggaag caggggatct tgtaggctgt acgactccaa
     2581 cgtgttccgg cacatctatc tgggcctgac cgtgatcctg ggcacagtgt ctatcctgct
     2641 gagcatcgcc gtgctgttca tcctgaagaa gaactacgtg tccaagcacc gctcttttat
     2701 cacaaagagg gagcgcacca tggtgagcac acggttccag aaggagaatt ataccacatc
     2761 cgaccacctg ctgcagccta actactggcc aggcaaggag acccagctgg acccagcttt
     2821 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2881 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2941 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     3001 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     3061 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3121 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3181 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3241 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3301 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3361 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3421 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3481 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3541 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3601 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3661 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3721 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3781 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3841 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3901 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3961 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     4021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4681 catgtt
//
