LOCUS       sequence-262724-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of SLC38A9 N term fragment (1-119).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Shen K, Sabatini DM
  TITLE     Ragulator and SLC38A9 activate the Rag GTPases through noncanonical 
            GEF mechanisms.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 
            10.1073/pnas.1811727115. Epub 2018 Sep 4.
  PUBMED    30181260
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1811727115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-09-18- 18"; volume: "115"; issue: "38"; pages: 
            "9545-9550"
COMMENT     Sequence Label: [pSK581] pRK5 HA-SLC38A9N
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             64..90
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      514..523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    527..608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(564..583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     618..637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atcgattgaa ttcccgaacc gacagtcggt ctcttcacca aggccattcg cgccaccatg
       61 ggctacccct atgatgtgcc tgactacgca gatctcaatg gtggtggtgg tgggtcgacg
      121 atggctaaca tgaacagtga ctcaagacat ctgggaacaa gcgaggtgga ccacgagcgg
      181 gaccctggcc ctatgaacat tcagtttgag ccctccgacc tgcggtctaa gagacccttc
      241 tgcatcgaac ctaccaacat tgtgaacgtc aatcacgtga tccagagggt cagtgatcat
      301 gcatcagcca tgaataagcg aattcactac tattcccggc tgaccacacc cgctgacaaa
      361 gcactgatcg cccctgatca tgtggtccca gcacccgagg aatgttacgt gtatagccct
      421 ctgggatccg cctacaagct gcagagctac accgaggggt atggaaaaaa cacatcctga
      481 gcggccgcta agtaagtaag gatccccagc ttggccgcca tggcccaact tgtttattgc
      541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtat
//
