LOCUS       sequence-262725-         527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of SLC38A9 N term fragment (1-119).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Shen K, Sabatini DM
  TITLE     Ragulator and SLC38A9 activate the Rag GTPases through noncanonical 
            GEF mechanisms.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 
            10.1073/pnas.1811727115. Epub 2018 Sep 4.
  PUBMED    30181260
REFERENCE   2  (bases 1 to 527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1811727115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-09-18- 18"; volume: "115"; issue: "38"; pages: 
            "9545-9550"
COMMENT     Sequence Label: [pSK104] pET28 SLC38A9N-His
FEATURES             Location/Qualifiers
     source          1..527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             402..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(472..490)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gaaggagata taccatggca aacatgaaca gcgacagccg tcacctgggc acgagcgaag
       61 ttgaccacga acgcgatccg ggtccgatga atatccaatt tgaaccgtca gatctgcgtt
      121 cgaaacgccc gttttgcatc gaaccgacca acattgttaa cgtcaatcat gtgatccagc
      181 gtgttagcga ccacgcatct gctatgaaca aacgtattca ttactacagc cgcctgacca
      241 cgccggcgga taaagccctg attgcaccgg accacgtggt tccggctccg gaagaatgtt
      301 atgtgtacag tccgctgggc tccgcgtata aactgcaaag ctataccgaa ggctacggta
      361 aaaacacctc tggtggcggt ggcagcgcgg ccgcactcga gcaccaccac caccaccact
      421 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggt
//
