LOCUS       sequence-262726-        1448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of c7orf59 and HBXIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Shen K, Sabatini DM
  TITLE     Ragulator and SLC38A9 activate the Rag GTPases through noncanonical 
            GEF mechanisms.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 
            10.1073/pnas.1811727115. Epub 2018 Sep 4.
  PUBMED    30181260
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1811727115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-09-18- 18"; volume: "115"; issue: "38"; pages: 
            "9545-9550"
COMMENT     Sequence Label: [pSK24] pACYCDuet His-c7orf59|HBXIP
FEATURES             Location/Qualifiers
     source          1..1448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..35
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             112..135
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     507..526
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        507..525
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    526..550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             884..928
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(966..984)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      980..1027
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
       61 ttaataagga gatataccat gggcagcagc catcaccatc atcaccacag cctggaagtt
      121 ctgttccagg ggcccggatc catgacttct gcgctgaccc aggggctgga gcgaatccca
      181 gaccagctcg gctacctggt actgagtgaa ggtgcagtgc tggcgtcatc tggggacctg
      241 gagaatgatg agcaggcagc cagtgccatc tctgagctgg tcagcacagc ctgcggtttc
      301 cggctgcacc gcggcatgaa tgtgcccttc aagcgcctgt ctgtggtctt tggagaacac
      361 acactgctgg tgacggtgtc aggacagagg gtgtttgtgg tgaagaggca gaaccgaggt
      421 cgggagccca ttgatgtctg agcggccgca taatgcttaa gtcgaacaga aagtaatcgt
      481 attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga
      541 taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac atatgatgga
      601 ggcgaccttg gagcagcact tggaagacac aatgaagaat ccctccattg ttggagtcct
      661 gtgcacagat tcacaaggac ttaatctggg ttgccgcggg accctgtcag atgagcatgc
      721 tggagtgata tctgttctag cccagcaagc agctaagcta acctctgacc ccactgatat
      781 tcctgtggtg tgtctagaat cagataatgg gaacattatg atccagaaac acgatggcat
      841 cacggtggca gtgcacaaaa tggcctcttg actcgagtct ggtaaagaaa ccgctgctgc
      901 gaaatttgaa cgccagcaca tggactcgtc tactagcgca gcttaattaa cctaggctgc
      961 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     1021 ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa
     1081 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     1141 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     1201 gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     1261 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1321 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1381 tatttgccca tagtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1441 aaactggt
//
