LOCUS       sequence-262728-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of p14 and MP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Shen K, Sabatini DM
  TITLE     Ragulator and SLC38A9 activate the Rag GTPases through noncanonical 
            GEF mechanisms.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 
            10.1073/pnas.1811727115. Epub 2018 Sep 4.
  PUBMED    30181260
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1811727115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-09-18- 18"; volume: "115"; issue: "38"; pages: 
            "9545-9550"
COMMENT     Sequence Label: [pSK131] pCOLADuet p14|MP1
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..31
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     524..543
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        524..542
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    543..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1006..1050
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tataggggaa ttgtgagcgg ataacaattc ccctgtagaa ataattttgt ttaactttaa
       61 taaggagata taccatggga atgctgcgtc ccaaggcttt gacgcaggtg ctaagccaag
      121 ccaacactgg aggagtccag agcaccctgc tgctgaataa tgagggatcg ctgctggcct
      181 actccggtta tggggacaca gatgcccggg tcactgcggc catcgccagt aacatctggg
      241 ccgcgtatga taggaacggg aaccaagcgt ttaatgaaga cagtctcaaa tttatcctga
      301 tggactgcat ggagggccgt gtagccatta cgagggtggc caaccttctg ctatgtatgt
      361 atgccaagga gaccgtaggc ttcggaatgc tcaaggctaa ggcccaggcc ctggtgcagt
      421 acctggagga acccctcacc caagtagcag catcataagc ggccgcataa tgcttaagtc
      481 gaacagaaag taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata
      541 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
      601 gatatacata tggcggatga tctaaagcga ttcctgtaca aaaagttgcc aagtgttgaa
      661 gggctccatg caattgttgt gtcagataga gacggggtgc cggttattaa agtggctaac
      721 gacagtgccc cagagcacgc cctgagacct ggcttcctat ccacgtttgc cctcgcaaca
      781 gaccaaggca gcaaactcgg actttcaaaa aacaaaagca tcatctgcta ctataacacc
      841 taccaggtgg ttcagttcaa tcgtttgcct ctggtggtga gtttcatagc cagcagcagt
      901 gccaacacag gactaattgt cagcctagaa aaggagctag ctccattatt tgaagaactg
      961 ataaaagttg tggaagtgtc tactagtagt tgactcgagt ctggtaaaga aaccgctgct
     1021 gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaa
//
