LOCUS       sequence-262729-        1284 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Nprl2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Shen K, Valenstein ML, Gu X, Sabatini DM
  TITLE     Arg-78 of Nprl2 catalyzes GATOR1-stimulated GTP hydrolysis by the 
            Rag GTPases.
  JOURNAL   J Biol Chem. 2019 Feb 22;294(8):2970-2975. doi: 
            10.1074/jbc.AC119.007382. Epub 2019 Jan 16.
  PUBMED    30651352
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.AC119.007382"; journalName: "J Biol Chem"; date: 
            "2019-02-22- 22"; volume: "294"; issue: "8"; pages: "2970-2975"
COMMENT     Sequence Label: [pSK600] pRK5 HA-Nprl2(R78A)
FEATURES             Location/Qualifiers
     source          1..1284
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..89
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcgattgaat tcccgaaccg acagtcggtc tcttcaccaa ggccattcgc gccaccatgg
       61 gctaccccta tgatgtgcct gactacgcag atctcaatgg tggtggtggt gggtcgacga
      121 tgggcagcgg ctgccgcatc gaatgcatat tcttcagcga gttccacccc acgctgggac
      181 ccaagatcac ctatcaggtc cctgaagact tcatctcccg agagctgttt gacacagtcc
      241 aagtgtacat catcaccaag ccagagctgc agaacaagct tatcactgtc acagctatgg
      301 aaaagaagct gatcggctgt cctgtgtgca tcgaacacaa gaagtacagc gcgaatgctc
      361 tcctcttcaa cctgggcttc gtgtgtgatg cccaggccaa gacctgcgcc ctcgagccca
      421 ttgttaaaaa gctggctggc tatctgacca cactagagct agagagcagc ttcgtgtcca
      481 tggaggagag caagcagaag ttggtgccca tcatgaccat cttgctggag gagctaaatg
      541 cctcaggccg gtgcactctg cccattgatg agtccaacac catccacttg aaggtgattg
      601 agcagcggcc agaccctccg gtggcccagg agtatgatgt acctgtcttt accaaagaca
      661 aggaggattt cttcaactca cagtgggacc tcactacaca acaaatcctg ccctacattg
      721 atgggttccg ccacatccag aagatttcag cagaggcaga tgtggagctc aacctggtgc
      781 gcattgctat ccagaacctg ctgtactacg gcgttgtgac actggtgtcc atcctccagt
      841 actccaatgt atactgccca acgcccaagg tccaggacct ggtagatgac aagtccctgc
      901 aagaggcatg tctatcctac gtgaccaagc aagggcacaa gagggccagt ctccgggatg
      961 tgttccagct atactgcagc ctgagccctg gcactaccgt gcgagacctc attggccgcc
     1021 acccccagca gctgcagcat gttgatgaac ggaagctgat ccagttcggg cttatgaaga
     1081 acctcatcag gcgactacag aagtatcctg tgcgggtgac tcgggaagag cagagccacc
     1141 ctgcccggct ttatacaggc tgccacagct atgacgagat ctgctgcaag acaggcatga
     1201 gctaccatga gctggatgag cggcttgaaa atgaccccaa catcatcatc tgctggaagt
     1261 gagcggccgc taagtaagta agga
//
