LOCUS       sequence-262731-        2641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, 
            lacZ blue-white screening. Release parts to assemble transcription 
            units with BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: pUAP4
FEATURES             Location/Qualifiers
     source          1..2641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(184..227)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     341..360
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     555..577
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     569..586
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     570..586
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    587..643
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(656..672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(656..672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(669..691)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    680..696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(704..710,711..728,729..734))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    749..770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      844..901
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(925..942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1096..1684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1176..1195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1867..1961)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1982..2641)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2556..2575
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
      241 cgaagagcca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
      301 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
      361 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      421 atcaggcgct catccgccat tcaggctgcg caactgttgg gaagggcgat cggcgcaggc
      481 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      541 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg
      601 tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat
      661 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      721 gcatatagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      781 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctggctctt cgagaccctg
      841 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      901 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1081 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1501 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1861 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1921 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1981 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2041 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2101 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2161 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2221 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2281 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2341 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2401 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2461 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2521 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2581 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2641 t
//
