LOCUS       sequence-262754-        2280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasma Membrane targeting protein for C-term fusion with CDS12 
            without linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_linker-Lti6b
FEATURES             Location/Qualifiers
     source          1..2280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(195..252)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      508..551
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(555..573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             735..1394
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(801..820)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1415..1509
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2181..2200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc
      181 ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga caccttgccc
      241 ttttttgccg gactgcaggg tctcgttcgg gagctgcggc agctgccgct gcggcagcgg
      301 ccgaattcaa gcgcttgaaa atgagtacag ccactttcgt agagattatt cttgctatca
      361 tcttgcctcc tctcggcgtc tttctcaaat ttggttgcaa ggttgagttt tggatatgtt
      421 tgattttgac gctgtttggt tatcttcccg gaatccttta cgctctttat atcatcacct
      481 tttgatgagc tttgagaccg aattccagaa atcatcctta gcgaaagcta aggatttttt
      541 ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      601 ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt tccaactttc
      661 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      721 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      781 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      841 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      901 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
      961 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1021 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1081 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1141 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     1201 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1261 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1321 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1381 agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga tccagtaatg
     1441 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     1501 ggtgagaatc caagcctcga gctgtcagac caagtttact catatatact ttagattgat
     1561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctgtggaaa
//
